Journal for Veterinary Medicine, Biotechnology and Biosafety
Volume 1, Issue 2, June 2015, Pages 15–21
ISSN 2411-3174 (print version) ISSN 2411-0388 (online version)
INfLuENCE Of PHYTONuTRIENT ‘VITASTIm’ ON CHICKEN muCOSAL ImmuNITY AfTER INfECTION wITH LOw-PATHOLOGICAL AVIAN INfLuENZA VIRuS
Stegniy B. T., Shutchenko P. O., Muzyka D. V., Kovalenko L. V., Medvid K. O., Stegniy M. Yu., Obukhovska O. V.
Lillehoj H. S.
Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Beltsville Agricultural Research Center, Beltsville, Maryland, USA
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Citation for print version: Stegniy B. T., Shutchenko P. O., Muzyka D. V., Kovalenko L. V., Medvid K. O., Stegniy M. Yu., Obukhovska O. V. and Lillehoj H. S. (2015) ‘Influence of phytonutrient ‘Vitastim’ on chicken mucosal immunity after infection with low-pathological avian influenza virus’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 1(2), pp. 15–21.
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Citation for online version: Stegniy B. T., Shutchenko P. O., Muzyka D. V., Kovalenko L. V., Medvid K. O., Stegniy M. Yu., Obukhovska O. V. and Lillehoj H. S. (2015) ‘Influence of phytonutrient ‘Vitastim’ on chicken mucosal immunity after infection with low-pathological avian influenza virus’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 1(2), pp. 15–21. Available at: http://jvmbbs.kharkov.ua/archive/2015/volume1/issue2/oJVMBBS_2015012_015-021.pdf
Summary. The aim of our study was to investigate the immunostimulatory effect of phytonutrient ‘Vitastim’ on the immune response after avian infection with low pathogenic avian influenza virus.
It was conducted three groups of experimental chickens of 6 week old (21 birds in each group) to study the mucosal immunity of chickens after experimental infection with low-pathological avian influenza virus, and application of phytonutrient ‘Vitastim’. Cryostat sections of 7-μm thickness were prepared, and detection of cells was done using unlabeled primary monoclonal antibodies against the CD4, CD8, IgM, IgG, IgA, macrophage antigens, and a commercially available staining kit. As a negative control, slides were incubated with PBS instead of the monoclonal antibodies. Sections were counterstained with hematoxylin and mounted with Canada balsam. Statistical analysis was performed using SPSS 17.0 software for Windows.
By the results of immunohistochemical research of influence of immunostimulating phytogenous preparation ‘Vitastim’ on organism of chickens there was determined that given preparation more actively influence on humoral cell of immune reaction in norm and at lowly pathogenic avian influenza (caeca, trachea, lungs) that testified more intensive formation and accumulation of B-lymphocytes which produce immunoglobulins. At research of spleen, there was determined amplified proliferation of T-lymphocytes, macrophages that characterize the activation of cell immune reaction.
The results of immunohistochemical studies of inner organs of chickens of 3 research groups, it was established the influence of the phytonutrient ‘Vitastim’ on humoral immune system, as evidenced by high levels of IgM, IgG, IgA. ‘Vitastim’ had stimulating impact on state of cell immunity, however, as evidenced by the low levels of CD4, macrophages, in chickens of experimental groups compared to the intact birds, cell immunity does not play a significant role in the pathogenesis of LPAI.
Keywords: avian influenza virus, cell-, and humoral-mediated immune response, immunohistochemistry, low pathogenic avian influenza
Kogut, M. H. and Klasing, K. (2009) ‘An immunologist’s perspective on nutrition, immunity, and infectious diseases: introduction and overview’, The Journal of Applied Poultry Research, 18(1), pp. 103–110. doi: http://dx.doi.org/10.3382/japr.2008-00080
Lee, S.‑H., Lillehoj, H. S., Heckert, R. A., Cho, S.‑M., Tuo, W., Lillehoj, E. P., Chun, H.‑K. and Park, H.‑J. (2008) ‘Immune enhancing properties of safflower leaf (Carthamus tinctorius) on chicken lymphocytes and macrophages’, The Journal of Poultry Science, 45(2), pp. 147–151. doi: http://dx.doi.org/10.2141/jpsa.45.147
Lee, S.-H., Lillehoj, H. S., Jang, S. I., Lee, K.‑W., Yancey, R. J. and Dominowski, P. (2010) ‘The effects of a novel adjuvant complex/Eimeria profilin vaccine on the intestinal host immune response against live E. acervulina challenge infection’, Vaccine, 28(39), pp. 6498–6504. doi: http://dx.doi.org/10.1016/j.vaccine.2010.06.116
Lee, S. H., Lillehoj, H. S., Jang, S. I., Lee, K. W., Park, M. S., Bravo, D. and Lillehoj, E. P. (2011) ‘Cinnamaldehyde enhances in vitro parameters of immunity and reduces in vivo infection against avian coccidiosis’, British Journal of Nutrition, 106(06), pp. 862–869. doi: http://dx.doi.org/10.1017/s0007114511001073
Krasnikov, G. A., Truskova, T. Yu., Bondar’, L. A., Kiprich, V. V. and Matsenko, E. V. (2002) ‘Toxicological evaluation of phytopreparation Vitastim’ [Toksykolohichna otsinka fitopreparatu Vitastym]. Veterinary Medicine [Veterynarna medytsyna], 80, pp. 331–337. [in Ukrainian]