Issue 3

Journal for Veterinary Medicine, Biotechnology and Biosafety

Volume 1, Issue 3, September 2015, Pages 20–26

ISSN 2411-3174 (print version) ISSN 2411-0388 (online version)

Brucellosis — the current situation in Poland

Iwaniak W., Szulowski K.

Department of Microbiology, National Veterinary Research Institute, Pulawy, Poland, email:

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Citation for print version: Iwaniak, W. and Szulowski, K. (2015) ‘Brucellosis — the current situation in Poland’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 1(3), pp. 20–26.

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Citation for online version: Iwaniak, W. and Szulowski, K. (2015) ‘Brucellosis — the current situation in Poland’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 1(3), pp. 20–26. Available at:

Summary. The surveys of animals for brucellosis in Poland are primarily based on serological tests. The examinations are performed by regional laboratories using RBT. In the case of positive results obtained in this test the samples are examined in SAT and CFT. The definitive confirmatory investigations are conducted by the National Reference Laboratory for Brucellosis in the Department of Microbiology of NVRI in Pulawy, which additionally uses Coombs’ test, 2-Me test and ELISA. In the paper results of the examination of cattle in Poland in the years 2005–2014 are shown. Each year during examination period 130–420 cows were involved in confirmatory investigations and 6–34 animals were classified as positive for brucellosis. In bacteriological examinations of samples from seropositive cows, B. abortus has never been isolated. Only in 2006 B. suis biovar 2 has been recovered from the bovine internal organs. B. melitensis has never been reported and according to the Commission Decision from 2006, Poland has been regarded as ‘Brucellosis officially free country’. At the moment, the aim of the ongoing testing is to maintain the B. melitensis free country status. Sheep and goats are tested using RBT. Samples reacting positive in the RBT are retested in the National Reference Laboratory for Brucellosis (NRL) using again RBT and CFT. Up to now all samples tested in NRL were finally regarded as negative. Ovine epididymitis (B. ovis) has been also reported in Poland. Diagnosis of the disease is based on the serological examination by complement fixation test (CFT) using the antigen obtained from the rough strain of B. ovis REO198. Similarly, examinations based on the antigen obtained from B. canis are conducted in dogs (rapid slide agglutination test – RSAT and slow agglutination test – SAT), but brucellosis in this species of animals has never been confirmed in Poland. Regarding the situation in porcine brucellosis, there were some outbreaks several years ago and active monitoring of pigs is performed. In general, positive results are mainly connected with false positive serological reactions (FPSR) due to Y. enterocolitica O:9. There is no active monitoring of brucellosis in the wildlife animals in Poland but samples are taken during the hunting season and tested for scientific purposes. When testing 4407 samples of the wild boar sera, 1077 sera reacted positive in ELISA. Also research done on hare sera several years ago indicated very similar percentage of the positives. These data show that the wildlife is a huge reservoir of the Brucella and it could play a role in an epidemiology of brucellosis also in domestic animals.

Keywords: Brucella, bovine brucellosis, porcine brucellosis, wildlife, B. suis biovar 2


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