Issue 2
Journal for Veterinary Medicine, Biotechnology and Biosafety
Volume
2, Issue 2, July 2016, Pages 5–8
ISSN 2411-3174 (print version) ISSN 2411-0388
(online version)
The dynamics of the specific
immunity formation in sheep under the inactivated vaccines against Contagious
Agalactia of Sheep and Goats
Stegniy
B. T., Obuchovska O. V., Glebova K. V.
National
Scientific Center ‘Institute of Experimental and Clinical Veterinary
Medicine’, Kharkiv, Ukraine, e-mail: katerinaglebova25@gmail.com
Bogach
M. V.
Odesa
Research Station of the National Scientific Center ‘Institute of
Experimental and Clinical Veterinary Medicine’, Odesa, Ukraine
Bogach D. M.
Kharkiv
State Zooveterinary Academy, Kharkiv, Ukraine
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PDF (print version)
Citation for print version: Stegniy, B. T., Obuchovska, O. V., Glebova,
K. V., Bogach, M. V. and Bogach, D. M. (2016) ‘The dynamics of the specific
immunity formation in sheep under the inactivated vaccines against Contagious
Agalactia of Sheep and Goats’, Journal
for Veterinary Medicine, Biotechnology and Biosafety, 2(2),
pp. 5–8.
Download
PDF (online version)
Citation for online version: Stegniy, B. T., Obuchovska, O. V., Glebova,
K. V., Bogach, M. V. and Bogach, D. M. (2016) ‘The dynamics of the
specific immunity formation in sheep under the inactivated vaccines against
Contagious Agalactia of Sheep and Goats’, Journal for Veterinary Medicine, Biotechnology and Biosafety.
[Online] 2(2), pp. 5–8. Available at: http://jvmbbs.kharkov.ua/archive/2016/volume2/issue2/oJVMBBS_2016022_005-008.pdf
Summary. The
aim of the research is constructing and efficiency studying of inactivated
vaccine against contagious agalactia of sheep and goats. The bacterial mass of Mycoplasma
agalactiae S-11 production culture, obtained on artificial media, has been used as initial product. The vaccine contains 60%
of formalin-inactivated mycoplasmas cell suspension (6.107 cfu in a dose) in
sterile phosphate-buffered saline and 40% of aluminum hydroxide. Vaccination of
sheep in the sheep farms of Odessa region by ‘Ahavak’ vaccines (S.N.
‘Institutul Pasteur’ S.A. Romania) and NSC ‘IECVM’
using different schemes. Animals were studied using bacteriological,
serological and biochemical methods. The vaccine from Mycoplasma agalactiae S-11
strain does not cause the local reaction and immunosuppression on organisms of
sensitive livestock animals. The level of protective antibodies among research
groups of animals for NSC ‘IECVM’ vaccine was 3.54
(3.47–3.61) log2 at the 30th day after
revaccination and 3.59 (3.52–3.64) log2 for
‘Ahavak’ vaccine. The antibodies against Mycoplasma agalactiae were
registered in 14 heads of the control group (n=50) and accounted for 1.31
(0.00–2.86) log2. It was found that disease signs and presence
of pathogen are absent in conjoined holding animals which were vaccinated
against contagious agalactia of sheep and goats by NSC ‘IECVM’ and
Romanian vaccines as compared to the animals with clinical sings of the
disease. The intracutaneous injection of inactivated vaccine against contagious
agalactia of sheep and goats (NSC ‘IECVM’) two times provides 100%
protection of sensitive animals against clinical signs of the disease. It is
harmless, areactogenic, with immunogenic and protective properties equally to
the Romanian vaccine.
Keywords: contagious
agalactia, sheep, goats, inactivated vaccines, ELISA
References:
Ariza-Miguel, J., Rodriguez-Lazaro, D. and Hernandez, M.
(2012) ‘A survey of Mycoplasma agalactiae in dairy sheep farms in
Spain’, BMC Veterinary Research, 8(1), p. 171. http://dx.doi.org/10.1186/1746-6148-8-171
Kumar, A., Rahal, A., Chakraborty, S.,
Verma, A. K. and Dhama, K. (2014) ‘Mycoplasma
agalactiae, an etiological agent of contagious Agalactia in small
ruminants: A review’, Veterinary Medicine International,
2014(Article ID 286752), pp. 1–13. http://dx.doi.org/10.1155/2014/286752
Madanat, A., Zendulkova, D. and
Pospišil, Z. (2001) ‘Contagious Agalactia of sheep and goats. A review’, Acta Veterinaria Brno, 70(4),
pp. 403–412. http://dx.doi.org/10.2754/avb200170040403
Poumarat, F., Gautier-Bouchardon, A. V.,
Bergonier, D., Gay, E. and Tardy, F. (2016) ‘Diversity and
variation in antimicrobial susceptibility patterns over time in Mycoplasma
agalactiae isolates collected from sheep and goats in France’, Journal
of Applied Microbiology, 120(5), pp. 1208–1218. http://dx.doi.org/10.1111/jam.13083
Veterinary Record (2014) ‘Contagious
Agalactia pathogen confirmed in Wales’, Veterinary Record,
175(19), pp. 468–468. http://dx.doi.org/10.1136/vr.g6771
