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Issue 3

Journal for Veterinary Medicine, Biotechnology and Biosafety

Volume 2, Issue 3, October 2016, Pages 12–18

ISSN 2411-3174 (print version) ISSN 2411-0388 (online version)

PHYSIOLOGICAL AND BIOCHEmICAL mECHANISmS Of CONTACT INTERACTION Of NANOPARTICLES Of GOLD WITH BACILLUS ANTHRACIS VACCINE STRAIN STERNE 34f2 CELLS

Roman’ko M. E.

National Scientific Center ‘Institute of Experimental and Clinical Veterinary Medicine’, Kharkiv, Ukraine, e-mail: toxi-lab@ukr.net

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Citation for print version: Roman’ko, M. E. (2016) ‘Physiological and biochemical mechanisms of contact interaction of nanoparticles of gold with Bacillus anthracis vaccine strain Sterne 34F2 cells’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 2(3), pp. 12–18.

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Citation for online version: Roman’ko, M. E. (2016) ‘Physiological and biochemical mechanisms of contact interaction of nanoparticles of gold with Bacillus anthracis vaccine strain Sterne 34F2 cells’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 2(3), pp. 12–18. Available at: http://jvmbbs.kharkov.ua/archive/2016/volume2/issue3/oJVMBBS_2016023_012-018.pdf

Summary. The article presents the results of experimental studies of mechanisms of Aurum nanoparticles contact interaction on Bacillus anthracis vaccine strain. This research was aimed to study the physiological and biochemical mechanisms of influence of Aurum nanoparticles (AuNP) on B. anthracis cells (strain Sterne 34F2 — productive vaccine strain). The cell cultures biomass of B. anthracis (strain Sterne 34F2) was used as a model for our experiments. The sterile aqueous dispersion of Aurum nanoparticles with average size 19.0±0.9 nm and initial concentration of 19.3 μg/cm³ for the metal was used for accumulation of B. anthracis (strain Sterne 34F2) biomass and with average size of 30.0±0.6 nm in initial concentration of 38.6 μg/cm³ and 77.2 μg/cm³ by the metal — in experiments on the study of physiological and biochemical mechanisms of interaction of cells. The effectiveness of the interaction of B. anthracis cells (strain Sterne 34F2) with AuNP was evaluated by a membrane filter method with a followed by determination of optical density changes of mixture on a spectrophotometer. The value of H⁺‑ATPase activity (KF 3.6.3.6) in total membrane fraction (TMF) bacterial cells was recorded by the accumulation of inorganic phosphorus (P_i). Respiratory activity (RA) of B. anthracis was measured by oxygen of electrode Clarks type. Measured parameter was the maximum speed of reducing the concentration of oxygen in the environment measurement, reduced to a unit of bacterial biomass. According to results of the impact of nanoparticles of Aurum (gold, AuNP) for basic the physiological and biochemical indicators of B. anthracis cells (strain Sterne 34F2) appeared promising metal nanoparticles in a concentration range 2.90–8.69 μg/cm³ for metal, as indicated by the presence of ATPase and respiratory activity stimulation and is consistent with the highest accumulation of cells research AuNP along with the intensification of proliferative activity of B. anthracis cells. The mechanisms of gold accumulation in B. anthracis cells have the metabolism-dependent nature which is characteristic only active metabolized cells, and desplayed within our experiment involving certain assets metabolism (determining role of the transmembrane potential and its generators — ATPase, respiratory activity (RA)) in the overall regulation of the metabolic system and functional organization (proliferation activity) of the bacterial cells.

Keywords: Bacillus anthracis, bacterial cell, Aurum (gold) nanoparticles, contact interaction, proliferative activity of H⁺‑ATPase activity, respiratory activity

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