Issue 4

Journal for Veterinary Medicine, Biotechnology and Biosafety

Volume 2, Issue 4, December 2016, Pages 5–10

ISSN 2411-3174 (print version) ISSN 2411-0388 (online version)

Estimation of inbreeding depression by CAPN1, CAST, GH, GHR and CYP3A genes for quantitative and reproduction traits in SEVEN Aberdeen-Angus lines

Fedota O. M. 1 , Ruban S. Yu. 2 , Lysenko N. G. 1, *, Goraichuk I. V. 3 , Kolisnyk A. I. 4 , Tyzhnenko T. V. 1

1 V. N. Karazin Kharkiv National University, Kharkiv, Ukraine, e-mail:

2 Institute of Animal Breeding and Genetics nd. a. M. V. Zubets of NAAS, Chubynske, Ukraine

3 National Scientific Center ‘Institute of Experimental and Clinical Veterinary Medicine’, Kharkiv, Ukraine

4 PE ‘Agrofirma Svitanok’, Novoselivka, Ukraine

* Scientific adviser — Fedota O. M., Dr. Sci. (Biol.), Prof.

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Citation for print version: Fedota, O. M., Ruban, S. Yu., Lysenko, N. G., Goraichuk, I. V., Kolisnyk, A. I. and Tyzhnenko, T. V. (2016) ‘Estimation of inbreeding depression by CAPN1, CAST, GH, GHR and CYP3A genes for quantitative and reproduction traits in seven Aberdeen-Angus lines’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 2(4), pp. 5–10.

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Citation for online version: Fedota, O. M., Ruban, S. Yu., Lysenko, N. G., Goraichuk, I. V., Kolisnyk, A. I. and Tyzhnenko, T. V. (2016) ‘Estimation of inbreeding depression by CAPN1, CAST, GH, GHR and CYP3A genes for quantitative and reproduction traits in seven Aberdeen-Angus lines’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 2(4), pp. 5–10. Available at:

Summary. High level of inbreeding has deleterious effects on animal productive and reproductive traits. The effect of inbreeding by CAPN316, CAST282, GH L127V, GHR F279Y, GHR A257G and CYP3A28 C994G markers on reproductive characteristics, weight dynamics (0–5 year age) and exterior traits was studied. Genealogical analysis showed that population includes seven lines of cattle. For the SNP genotyping, PCR-RFLP methods were set up. Testing deviation from the Hardy-Weinberg equilibrium was performed using Pearson’s chi-squared test. The estimation of inbreeding depression by FST coefficients for SNPs for traits studied was performed via multiple regression models with forward stepwise. The tested population was analyzed in Herdy-Weinberg equilibrium for all SNPs, except GHR F279Y. Lines Bryalhil Sau and Southome Extra considered as the best lines showed the lowest level of heterozygosity. Negative effect of level of inbreeding for different markers were seen for regression coefficients calf birth weight = –1.08±0.30 (GHR F279Y), body weight at 8 month = –0.71±0.11 (CAPN316), –0.49±0.11 (GHR F279Y) and 15 month = –0.54±0.04 (CAST282), –0.36±0.06 (GHR F279Y). Increased inbreeding had positive influence on regression coefficients for calf average daily gain = 0.86±0.26 (GH L127V), weight at 8 and 15 month = 1.43±0.13, 1.05±0.05 (GHR A257G) and exterior traits — shoulder, back and low back, rump and hindquarter = 1.09±0.11, 0.77±0.22, and 1.10±0.30 (CYP3A28 C994G).

Keywords: Aberdeen-Angus breed, inbreeding, SNPs, CAPN316, CAST282, GH L127V, GHR F279Y, GHR A257G, CYP3A28 C994G


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