Issue 1
Journal for Veterinary Medicine, Biotechnology and Biosafety
Volume
3, Issue 1, March 2017, Pages 5–11
ISSN 2411-3174 (print version) ISSN 2411-0388
(online version)
INDICATION AND SPECIES
DIffERENTIATION Of THE BABESIA
PROTOZOAN GENuS BY THE POLYmERASE CHAIN REACTION
Mokryi Yu. O., Ksyonz
I. M., Pochernyayev K. F., Kurman
A. F.
Institute
for Pig Breeding and Agroindustrial Production of
NAAS, Poltava, Ukraine, e-mail: pigbreeding@ukr.net
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PDF (print version)
Citation for print version: Mokryi, Yu. O., Ksyonz, I. M., Pochernyayev, K.
F. and Kurman, A. F. (2017) ‘Indication and
species differentiation of the Babesia protozoan genus by the polymerase chain reaction’,
Journal for Veterinary Medicine,
Biotechnology and Biosafety, 3(1), pp. 5–11.
Download
PDF (online version)
Citation for online version: Mokryi, Yu. O., Ksyonz, I. M., Pochernyayev, K.
F. and Kurman, A. F. (2017) ‘Indication and
species differentiation of the Babesia protozoan genus by the polymerase chain
reaction’, Journal for
Veterinary Medicine, Biotechnology and Biosafety. [Online] 3(1),
pp. 5–11. Available at: http://jvmbbs.kharkov.ua/archive/2017/volume3/issue1/oJVMBBS_2017031_005-011.pdf
Summary.
According to the information obtained from numerous literature sources, the Babesia protozoan
genus is known to be the most thoroughly studied as to
the structure of the gene encoding 18S of ribosomal
RNA. Based on the information provided by the international database of DNA
sequencing ‘GenBank’, conservative and
polymorphic sites of the above gene’s nucleotide sequences belonging to
different Babesia
species have been defined using MEGA4 software. The
conservative 18S gene region of rRNA
has been used to develop the system of oligonucleotide
primers allowed to indicate DNA of six representatives of Babesia species. Variable regions
of this gene were used to develop oligonucleotide
primers for Babesia canis, Babesia bovis, and Babesia divergens
representatives DNA identification on species-specific level. The multiplex PCR diagnostic test system has been developed for determining
presence of DNA of the representatives of six Babesia genus species (B. canis, B. divergens, B. caballi, B. major,
B. bigemina, B. bovis) in any biological sample. The research results
of biological samples confirmed the sensitivity and analytical specificity of
the developed PCR test system for the diagnostics and
differentiation of the certain Babesia genus species.
Keywords: Babesia,
multiplex PCR, test system, oligonucleotide primers,
indication, differentiation
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