Issue 1
Journal for Veterinary Medicine, Biotechnology and Biosafety
Volume
3, Issue 1, March 2017, Pages 12–16
ISSN 2411-3174 (print version) ISSN 2411-0388
(online version)
STAPHYLOCOCCAL CONTAmINATION
Of RAW mILK AND HANDmADE DAIRY PRODuCTS, WHICH ARE REALIZED
AT THE mARKETS Of uKRAINE
Kukhtyn M. D. 1 , Kovalenko
V. L. 2 , Pokotylo O. S. 1 , Horyuk Yu. V. 2 , Horyuk V. V. 3 , Pokotylo
O. O. 4
1 Ivan Puluj Ternopil National Technical University, Ternopil,
Ukraine; е-mail: kuchtyn@ya.ru
2 State Scientific Control Institute of
Biotechnology and strains, Kyiv, Ukraine
3 Podolsky State
Agricultural University, Kamyanets-Podilsky, Ukraine
4 I. Horbachevsky Ternopyl
State Medical University, Ternopil, Ukraine
Download
PDF (print version)
Citation for print version: Kukhtyn, M. D., Kovalenko, V. L., Pokotylo, O. S.,
Horyuk, Yu. V., Horyuk, V.
V. and Pokotylo, O. O. (2017) ‘Staphylococcal
contamination of raw milk and handmade dairy products, which are realized at
the markets of Ukraine’, Journal
for Veterinary Medicine, Biotechnology and Biosafety, 3(1),
pp. 12–16.
Download
PDF (online version)
Citation for online version: Kukhtyn, M. D., Kovalenko, V. L., Pokotylo, O. S.,
Horyuk, Yu. V., Horyuk, V.
V. and Pokotylo, O. O. (2017) ‘Staphylococcal
contamination of raw milk and handmade dairy products, which are realized at
the markets of Ukraine’, Journal
for Veterinary Medicine, Biotechnology and Biosafety.
[Online] 3(1), pp. 12–16. Available at: http://jvmbbs.kharkov.ua/archive/2017/volume3/issue1/oJVMBBS_2017031_012-016.pdf
Summary.
Milk and dairy products are placed in the first
group of risk because they can cause human diseases agent transmission. There
is a risk of alimentary staphylococcal toxicosis, in
the case of non-compliance with veterinary and sanitary requirements for the
primary processing of raw materials, manufacture and realization of finished
products. The aim of this study was to estimate a level of milk and dairy ‘home-made’
products contamination with coagulase positive staphylococci, to determine the
biological origin of Staphylococcus aureus, isolated from dairy products, and its ability
to produce enterotoxins. The article presents the results of raw milk and dairy
products study from private (‘home-made’) production, which are
selling at agro-food markets in Ukraine. Bacteria of the genus Staphylococcus are isolated from raw
milk and dairy products in 80.3–93.3% of cases. Coagulase positive
Staphylococcus species were isolated from sour cream — in 57.8% of
cases, which was 1.7 times more in comparison with the samples of raw milk
and was 2.5 times more in comparison with curd. Two biotypes of Staphylococcus aureus
(S. aureus
var. bovis
and S. aureus
var. hominis)
were isolated from samples of raw milk and dairy ‘home-made’ products,
which were realized at the markets. In this case, 46.6 ± 3.1%
of isolated cultures were classified as a biotype S. aureus
var. bovis,
and 53.4 ± 3.7% — to human ecovar.
It means that the main source of contamination of ‘home-made’ cream
by staphylococcus is people who do not follow proper hygiene and sanitation
during the producing process, namely: milking of cows, primary processing of
raw milk, production and realizing of dairy products. It was
found that S. aureus var. hominis had produced type A of enterotoxin in raw milk
and dairy products of private (‘home-made’) production, which,
generally, is cause of food toxicosis in humans.
Keywords: milk, dairy
products, staphylococci, biotypes, enterotoxins
References:
Efimochkina, N. R.
(2010) New bacterial pathogens in foods: an experimental study and
development of control systems with the use of methods of microbiological and
molecular genetic analysis [Novye bakterial’nye
patogeny v pishchevykh produktakh: eksperimental’noe
obosnovanie i razrabotka sistemy kontrolya s primeneniem metodov mikrobiologicheskogo i molekulyarno-geneticheskogo analiza]. The dissertation thesis for the scientific
degree of the doctor of biological
sciences. Moscow: The Scientific Research Institute of Nutrition of the Russian
Academy of Medical Sciences. [in Russian]
Jaber, N.
(2011) ‘Isolation and biotyping of Staphylococcus aureus
from white cheese in Basrah local markets’, Basrah Journal of Veterinary Research, 10(2),
pp. 55–66. Available at: http://www.iasj.net/iasj?func=article&aId=55025
Kukhtyn, M. D.
(2004) Veterinary and sanitary evaluation of cow’s milk on the content
of Staphylococcus aureus [Veterynarno-sanitarna
otsinka moloka koroviachoho nezbyranoho za vmistom zolotystoho
stafilokoku]. The dissertation thesis for the
scientific degree of the candidate of veterinary
sciences. Lviv: Lviv
National Academy of Veterinary Medicine named after S. Z. Gzhytskyj. [in Ukrainian]
Mayer, S. (1999) ‘Eingenschaften
von aus Kuhmilch isolaten Staphylokokken in Hinblick auf die Beurteilung von Milch’, Milchwissenschaft,
30, pp. 607–608
MEDTU
(Ministry of Economic Development and Trade of Ukraine) (2013) DSTU 7357:2013. Milk
and milk products. Methods of microbiological control [Moloko
ta molochni produkty. Metody mikrobiolohichnoho kontroliuvannia]. Kyiv: Minekonomrozvytku
Ukrainy
Morris, J. G. and Potter, M. E. (eds.) (2013) Foodborne
infections and intoxications. 4th ed.
London; Waltham, MA: Academic (Food Science and Technology). ISBN 9780124160415
Normanno, G.,
La Salandra, G., Dambrosio, A.,
Quaglia, N. C., Corrente, M.,
Parisi, A., Santagada, G.,
Firinu, A., Crisetti, E.
and Celano, G. V. (2007) ‘Occurrence,
characterization and antimicrobial resistance of enterotoxigenic
Staphylococcus aureus
isolated from meat and dairy products’, International Journal of Food
Microbiology, 115(3), pp. 290–296. http://dx.doi.org10.1016/j.ijfoodmicro.2006.10.049
Sudhanthiramani, S., Swetha, C. S. and Bharathy, S. (2015) ‘Prevalence of antibiotic
resistant Staphylococcus aureus from raw milk samples collected from the local
vendors in the region of Tirupathi, India’, Veterinary
World, 8(4), pp. 478–481. http://dx.doi.org10.14202/vetworld.2015.478-481
Thaker, H.,
Brahmbhatt, M. and Nayak, J.
(2013) ‘Isolation and identification of Staphylococcus aureus from milk and milk
products and their drug resistance patterns in Anand,
Gujarat’, Veterinary World, 6(1), p. 10–13. http://dx.doi.org10.5455/vetworld.2013.10-13
Wang, S.‑C., Wu, C.‑M., Xia, S.‑C.,
Qi, Y.‑H., Xia, L.‑N. and Shen, J.‑Z. (2009) ‘Distribution
of superantigenic toxin genes in Staphylococcus aureus isolates from milk
samples of bovine subclinical mastitis cases in two major diary production
regions of China’, Veterinary Microbiology, 137(3–4),
pp. 276–281. http://dx.doi.org10.1016/j.vetmic.2009.01.007
