Issue 1

Journal for Veterinary Medicine, Biotechnology and Biosafety

Volume 3, Issue 1, March 2017, Pages 17–20

ISSN 2411-3174 (print version) ISSN 2411-0388 (online version)

DImETHYL SuLfOXIDE AS THE STImuLANT ANTIGEN PRODuCING ACTIVITY Of THE CELL CuLTuRE fLK-BLV

Gorbatenko S. K. 1 , Stegniy M. Yu. 1 , Korneykov A. N. 1 , Myagkyh N. V. 1 , Magats D. Yu. 1 , Gorbatenko V. P. 2

1 National Scientific Center ‘Institute of Experimental and Clinical Veterinary Medicine’, Kharkiv, Ukraine, admin@vet.kharkov.ua

2 Kharkiv State Zooveterinary Academy, Kharkiv, Ukraine

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Citation for print version: Gorbatenko, S. K., Stegniy, M. Yu., Korneykov, A. N., Myagkyh, N. V., Magats, D. Yu. and Gorbatenko, V. P. (2017) ‘Dimethyl sulfoxide as the stimulant antigen producing activity of the cell culture FLK-BLV’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 3(1), pp. 17–20.

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Citation for online version: Gorbatenko, S. K., Stegniy, M. Yu., Korneykov, A. N., Myagkyh, N. V., Magats, D. Yu. and Gorbatenko, V. P. (2017) ‘Dimethyl sulfoxide as the stimulant antigen producing activity of the cell culture FLK-BLV’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 3(1), pp. 17–20. Available at: http://jvmbbs.kharkov.ua/archive/2017/volume3/issue1/oJVMBBS_2017031_017-020.pdf

Summary. The article observes a capability of the dimethyl sulfoxide (DMSO) use to stimulate the leukemia virus expression and antigen producing activity. Adhesive and proliferative activities of stimulated cell culture FLK-BLV were assessed at each passage of cell growth. Antigen producing activity was determined after the adaptation period in the 5–10 passages by RID. The addition of 1.0% dimethyl sulfoxide (DMSO) positively effects on the cells monolayer within 10 passages of the experiment. The activity of the experimental antigen produced on nutrient medium with the addition of the DMSO was higher 1.75 times than in control group.

Keywords: leukosis, cattle, cells culture, radial immunodiffusion, leukemia virus

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