Issue 3

Journal for Veterinary Medicine, Biotechnology and Biosafety

Volume 3, Issue 3, September 2017, Pages 5–9

ISSN 2411-3174 (print version) ISSN 2411-0388 (online version)


Gerilovych A. P., Limanska O. Yu., Kulyk I. A., Gorbatenko S. K.

National Scientific Center ‘Institute of Experimental and Clinical Veterinary Medicine’, Kharkiv, Ukraine, e-mail:

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Citation for print version: Gerilovych, A. P., Limanska, O. Yu., Kulyk, I. A. and Gorbatenko, S. K. (2017) ‘Studying of phylogenetic relationships of Leukemia virus with other retroviruses in cattle’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 3(3), pp. 5–9.

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Citation for online version: Gerilovych, A. P., Limanska, O. Yu., Kulyk, I. A. and Gorbatenko, S. K. (2017) ‘Studying of phylogenetic relationships of Leukemia virus with other retroviruses in cattle’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 3(3), pp. 5–9. Available at:

Summary. Bovine leukemia virus (BLV) is one of the retroviruses, which genetically, structurally and functionally related with viruses of human T‑cell leukemia. BLV is very convenient model for studying the pathogenesis of humans leukemia. Genomes of retroviruses is high variability due to lack of a mechanism of correct errors that occur when copying matrix during replication, and possible genetic recombination. In this regard, the study of the genetic variability of the virus is one of the major objective for biological monitoring. At this time molecular genetic analysis (polymerase chain reaction (PCR) is a necessary part of phylogenetic research. The aim of this work was to study the variability of the bovine leukemia virus, establishing phylogenetic relationships between isolates sequenced bovine leukemia virus, which circulates in farms of different regions in Ukraine, with other animals retroviruses. Was conducted the sampling of clinical material from cattle farms from different geographical regions in Ukraine and extracted proviral BLV DNA. Total received 831 samples of peripheral blood from cattle farms in Kharkiv region, 10 samples — Kirovohrad region; 10 samples — Donetsk region; 41 sample — Crimea, Simferopol region; 10 samples — Poltava region. Sequenced gene fragments of env sequences of bovine leukemia virus proviral DNA, circulating in different geographical regions in Ukraine. Established isolates of bovine leukemia virus, circulating in Ukraine, belonging to the Euro-Asian subtype. Proved genetic affinity of leukemia virus and bovine syncytial virus, Jembrana disease virus and bovine immunodeficiency virus.

Keywords: DNA, phylogeographic relationships, polymerase chain reaction, sequencing, retroviruses, virus bovine leukemia


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