Journal for Veterinary Medicine, Biotechnology and Biosafety
Volume 3, Issue 3, September 2017, Pages 10–17
ISSN 2411-3174 (print version) ISSN 2411-0388 (online version)
DEVELOPMENT OF PCR TEST SYSTEMS FOR SPECIES DIFFERENTIATION OF CHLAMYDIAL AGENTS IN BIRDS
Кsyonz І. М. 1, Korniyenko M. V. 1
1 Institute of Pig Breeding and Agroindustrial Production of the National Academy of Agrarian Sciences of Ukraine, Poltava, Ukraine, e-mail: email@example.com
2 National Scientific Center ‘Institute of Experimental and Clinical Veterinary Medicine’, Kharkiv, Ukraine, e-mail: firstname.lastname@example.org
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Citation for print version: Кsyonz, І. М. and Korniyenko, M. V. (2017) ‘Development of PCR test systems for species differentiation of chlamydial agents in birds’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 3(3), pp. 10–17.
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Citation for online version: Кsyonz, І. М. and Korniyenko, M. V. (2017) ‘Development of PCR test systems for species differentiation of chlamydial agents in birds’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 3(3), pp. 10–17. Available at: http://jvmbbs.kharkov.ua/archive/2017/volume3/issue3/oJVMBBS_2017033_010-017.pdf
Summary. According to the modern classification adopted by the 2nd European Meeting on Animal Chlamydioses and Zoonotic Implications (EMAC‑2), the genus Chlamydia contains three species of bird chlamydial agents — C. avium, C. gallinacea and C. psittaci. Three PCR test systems have been developed for indication and species differentiation of these bacteria. The bases for the developed diagnostics are three pairs of oligonucleotide primers designed and synthesized to flank the DNA fragments specific for each of the three bacteria species of the Chlamydia genus, which are pathogenic for birds. Analytical specificity of the developed PCR test systems was confirmed by the results of amplification of 18 biological materials samples, among them 13 being Chlamydia-containing, one of them contained C. avium, one — C. gallinacea, and 11 — C. psittaci. In addition Leptospira and Babesia DNA samples were tested. The developed PCR test systems for indication and species differentiation of bird Chlamydiosis agents permit to reliably study certain aspects of chlamydial infection.
Keywords: Chlamydioses, birds, МОМР, PCR test system, species differentiation, Chlamydia avium, Chlamydia gallinacea, Chlamydia psittaci
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