Issue 3

Journal for Veterinary Medicine, Biotechnology and Biosafety

Volume 3, Issue 3, September 2017, Pages 26–29

ISSN 2411-3174 (print version) ISSN 2411-0388 (online version)


Mazurenko V. R. 1, 2, Ponomarenko Т. О. 2

1 National Scientific Center ‘Institute of Biology and Medicine’ of the Taras Shevchenko National University of Kyiv, Kyiv, Ukraine, e-mail:

2 LLC ‘Center of Veterinary Diagnostics’, Kyiv, Ukraine

Download PDF (print version)

Citation for print version: Mazurenko, V. R. and Ponomarenko, Т. О. (2017) ‘Controlling of Mycoplasma bovis at a farm in Ukraine as a part of eradication program of Mastitis’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 3(3), pp. 26–29.

Download PDF (online version)

Citation for online version: Mazurenko, V. R. and Ponomarenko, Т. О. (2017) ‘Controlling of Mycoplasma bovis at a farm in Ukraine as a part of eradication program of Mastitis’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 3(3), pp. 26–29. Available at:

Summary. The article analyses the approaches to the diagnosis of mycoplasmal mastitis in cows. Despite decades of research in the field of etiology and pathogenesis of cattle mycoplasmosis, the diagnosis of this infection remains a topical issue in veterinary medicine. In time detection of infected cows, compliance with preventive measures in the milking parlors and in the newborncalves facilities, and the setting the order of milking are the main methods for preventing outbreaks of mycoplasmosis in farms. The necessity of proper sampling using and the order of the procedure for milking cows for the diagnosis of Mycoplasma bovis and other species of mycoplasmas are substantiated. Basing on the analysis, the following algorithm for determining the infectious status for Mycoplasma spp. and sampling for diagnosis, as well as the procedure for milking cows, was offered. The data obtained as a result of the study indicate the necessity to plan a set of preventive and therapeutic measures in the farms to prevent the infection of healthy animals with a contagious mycoplasmal infection.

Keywords: mastitis, Mycoplasma bovis, contagious pathogen, cows, Ukraine, control


Aebi, M., van den Borne, B., Raemy, A., Steiner, A., Pilo, P. and Bodmer, M. (2015) ‘Mycoplasma bovis infections in Swiss dairy cattle: a clinical investigation’, Acta Veterinaria Scandinavica, 57(1), p. 10.

Barkema, H., Green, M., Bradley, A. and Zadoks, R. (2009) ‘Invited review: The role of contagious disease in udder health’, Journal of Dairy Science, 92(10), pp. 4717–4729.

Fox, L. (2013) ‘Mycoplasma mastitis and prevention’, Proceedings of the 11th Western Dairy Management Conference, Reno, Nevada, USA, 6–8 March, pp. 5–16. Available at:

Fox, L., Kirk, J. and Britten, A. (2005) ‘Mycoplasma mastitis: A review of transmission and control’, Journal of Veterinary Medicine Series B, 52(4), pp. 153–160.

Houlihan, M., Veenstra, B., Christian, M., Nicholas, R. and Ayling, R. (2007) ‘Mastitis and arthritis in two dairy herds caused by Mycoplasma bovis’, Veterinary Record, 160(4), pp. 126–127.

Jones, H. and Simecka, J. (2003) ‘T lymphocyte responses are critical determinants in the pathogenesis and resistance to mycoplasma respiratory disease’, Frontiers in Bioscience, 8(4), pp. d930–945.

Justice-Allen, A., Trujillo, J., Goodell, G. and Wilson, D. (2011) ‘Detection of multiple Mycoplasma species in bulk tank milk samples using real-time PCR and conventional culture and comparison of test sensitivities’, Journal of Dairy Science, 94(7), pp. 3411–3419.

Lysnyansky, I., Freed, M., Rosales, R., Mikula, I., Khateb, N., Gerchman, I., van Straten, M. and Levisohn, S. (2016) ‘An overview of Mycoplasma bovis mastitis in Israel (2004–2014)’, The Veterinary Journal, 207, pp. 180–183.

Maunsell, F., Woolums, A., Francoz, D., Rosenbusch, R., Step, D., Wilson, D. and Janzen, E. (2011) ‘Mycoplasma bovis infections in cattle’, Journal of Veterinary Internal Medicine, 25(4), pp. 772–783.

Pothmann, H., Spergser, J., Elmer, J., Prunner, I., Iwersen, M., Klein-Jöbstl, D. and Drillich, M. (2015) ‘Severe Mycoplasma bovis outbreak in an Austrian dairy herd’, Journal of Veterinary Diagnostic Investigation, 27(6), pp. 777–783.

Rodríguez, F., Castro, P., Poveda, J., Afonso, A. and Fernández, A. (2015) ‘Immunohistochemical labelling of cytokines in calves infected experimentally with Mycoplasma bovis’, Journal of Comparative Pathology, 152(2–3), pp. 243–247.

Rossetti, B., Frey, J. and Pilo, P. (2010) ‘Direct detection of Mycoplasma bovis in milk and tissue samples by real-time PCR’, Molecular and Cellular Probes, 24(5), pp. 321–323.

Spergser, J., Macher, K., Kargl, M., Lysnyansky, I. and Rosengarten, R. (2013) ‘Emergence, re-emergence, spread and host species crossing of Mycoplasma bovis in the Austrian Alps caused by a single endemic strain’, Veterinary Microbiology, 164(3–4), pp. 299–306.

Stipkovits, L., Ripley, P., Varga, J. and Pálfi, V. (2000) ‘Clinical study of the disease of calves associated with Mycoplasma bovis infection", Acta Veterinaria Hungarica, 48(4), pp. 387–395.

Stukolkina, N. Ye. (2005) ‘Modern aspects of the biology of mycoplasma’ [Sovremennyye aspekty biologii mikoplazm], Herald of the Yaroslav the Wise Novgorod State University [Vestnik Novgorodskogo gosudarstvennogo universiteta imeni Yaroslava Mudrogo], 32, pp. 58–62. Available at: [in Russian]

Surýnek, J., Vrtková, I. and Knoll, A. (2016) ‘Mycoplasma bovis was not detected in milk from dairy cattle in the Czech Republic’, Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 64(1), pp. 165–168.

Zbinden, C., Pilo, P., Frey, J., Bruckmaier, R. and Wellnitz, O. (2015) ‘The immune response of bovine mammary epithelial cells to live or heat-inactivated Mycoplasma bovis’, Veterinary Microbiology, 179(3–4), pp. 336–340.