Journal for Veterinary Medicine, Biotechnology and Biosafety
Volume 3, Issue 4, December 2017, Pages 5–9
ISSN 2411-3174 (print version) ISSN 2411-0388 (online version)
DEVELOPMENT OF PCR-TEST SYSTEMS FOR SPECIES DIFFERENTIATION OF CHLAMIDIOSIS AGENTS IN FELIDAE FAMILY AND RODENTS ORDER
Кsyonz І. М. 1, Korniyenko M. V. 2
1 Institute of Pig Breeding and Agroindustrial Production of the National Academy of Agrarian Sciences of Ukraine, Poltava, Ukraine, e-mail: firstname.lastname@example.org
2 National Scientific Center ‘Institute of Experimental and Clinical Veterinary Medicine’, Kharkiv, Ukraine, e-mail: email@example.com
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Citation for print version: Кsyonz, І. М. and Korniyenko, M. V. (2017) ‘Development of PCR-test systems for species differentiation of Chlamidiosis agents in Felidae family and rodents order’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 3(4), pp. 5–9.
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Citation for online version: Кsyonz, І. М. and Korniyenko, M. V. (2017) ‘Development of PCR-test systems for species differentiation of Chlamidiosis agents in Felidae family and rodents order’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 3(4), pp. 5–9. Available at: http://jvmbbs.kharkov.ua/archive/2017/volume3/issue4/oJVMBBS_2017034_005-009.pdf
Summary. According to the current classification adopted at the 2nd European Symposium on Animal Chlamydiosis and Zoonotic Implications (EMAC‑2), pathogens causing chlamydioses in cats and rodents are Chlamydia genus bacteria of the three species: C. felis, C. caviae and C. muridarum. Three PCR test systems were developed for indication and species differentiation of the said bacteria. The basis for the developed diagnostics is the design and synthesis of three oligonucleotide primers pairs, flanking specific fragments of the chlamydial MOMP gene DNA pathogenic to cats and rodents. Analytical specificity of the developed PCR test systems is confirmed by the results of 13 biological materials samples amplification, the total of 13 ones being chlamydia-containing, namely: 4 samples contain C. felis, one — C. caviae, and one — C. muridarum. In addition to Chlamydia, DNA samples were taken from Leptospira and Babesia.
Keywords: cats, guinea pigs, mouse rodents, PCR-test system, species differentiation, Chlamydia felis, Chlamydia caviae, Chlamydia muridarum
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