Issue 4

Journal for Veterinary Medicine, Biotechnology and Biosafety

Volume 3, Issue 4, December 2017, Pages 16–22

ISSN 2411-3174 (print version) ISSN 2411-0388 (online version)


Fedota O. M. 1, Babalian V. O. 2, Mitiohlo L. V. 3, Mazniakov S. M. 2, Valilshchikov M. V. 2, Tyzhnenko T. V. 1, Ruban S. Yu. 4

1 V. N. Karazin Kharkiv National University, Kharkiv, Ukraine, e-mail:

2 Kharkiv Medical Academy of Postgraduate Education

3 SE RFNyva’, Khrystynivka, Ukraine

4 LLC ‘MPK Ekaterinoslavsky’, Dnipro, Ukraine

Download PDF (print version)

Citation for print version: Fedota, O. M., Babalian, V. O., Mitiohlo, L. V., Mazniakov, S. M., Valilshchikov, M. V., Tyzhnenko, T. V. and Ruban, S. Yu. (2017) ‘Bone mineral density in evaluation the productive traits and reproductive health of dairy cows’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 3(4), pp. 16–22.

Download PDF (online version)

Citation for online version: Fedota, O. M., Babalian, V. O., Mitiohlo, L. V., Mazniakov, S. M., Valilshchikov, M. V., Tyzhnenko, T. V. and Ruban, S. Yu. (2017) ‘Bone mineral density in evaluation the productive traits and reproductive health of dairy cows’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 3(4), pp. 16–22. Available at:

Summary. Importance of studying osteoporosis and bone mineral density is due to the search for understanding on the regulation of bone mineral density, finding the signaling pathways and novel therapeutic targets, clarification of the gene network of osteoporosis. Since it is not always possible to assess the influence of various factors on the normal variation in bone mineral density, it is advisable to use animals as models. The aim of this study was evaluation of the bone mineral density and its relation with reproductive and productive characteristics of lactating dairy cows. Statistical methods included Pearson’s chi-squared and t criteria, Pearson’s and Spearmen correlation coefficient r and ANOVA. We analyzed the bone tissue indicators, productive and reproductive traits of dairy cows. The relation between health and reproduction traits of dairy cows — somatic cell count in milk, the open day, efficiency of insemination and the bone mineral density of cows was demonstrated. Also we found that bone mineral density was in a negative correlation with between somatic cell count in milk (r = –0.67), duration of open day (r = –0.50). More insemination was required for animals with a lower level of bone mineral density (r = –0.67). The level of bone mineral density is notified as independent from age, number of lactations and pregnancy, and the productivity of cows — milk yield, milk fat, milk protein. In the absence of the influence of chemical agents on metabolism, the processes of pregnancy and lactation are not a reason for reducing the bone mineral density in a balanced and high-grade diet.

Keywords: bone mineral density, dairy cows, milk traits, osteoporosis, reproduction of cows, somatic cell count


Adlercreutz, H. and Mazur, W. (1997) ‘Phyto-oestrogens and Western Diseases’, Annals of Medicine, 29(2), pp. 95–120.

Alam, I., Padgett, L. R., Ichikawa, S., Alkhouli, M., Koller, D. L., Lai, D., Peacock, M., Xuei, X., Foroud, T., Edenberg, H. J. and Econs, M. J. (2014) ‘SIBLING family genes and bone mineral density: Association and allele-specific expression in humans’, Bone, 64, pp. 166–172.

Atramentova, L. A. and Utevskaya, A. M. (2008) Statistical methods in biology [Statisticheskie metody v biologii]. Gorlovka: Lіkhtar. ISBN 9789662129267. [in Russian]

Bone, H. G., Greenspan, S. L., McKeever, C., Bell, N., Davidson, M., Downs, R. W., Emkey, R., Meunier, P. J., Miller, S. S., Mulloy, A. L., Recker, R. R., Weiss, S. R., Heyden, N., Musliner, T., Suryawanshi, S., Yates, A. J. and Lombardi, A. (2000) ‘Alendronate and estrogen effects in postmenopausal women with low bone mineral density 1’, The Journal of Clinical Endocrinology & Metabolism, 85(2), pp. 720–726.

Boudin, E., Fijalkowski, I., Hendrickx, G. and Van Hul, W. (2016) ‘Genetic control of bone mass’, Molecular and Cellular Endocrinology, 432, pp. 3–13.

Coates, D. B., Dixon, R. M., Murray, R. M., Mayer, R. J. and Miller, C. P. (2016) ‘Bone mineral density in the tail-bones of cattle: effect of dietary phosphorus status, liveweight, age and physiological status’, Animal Production Science, 56(12), pp. 2054–2059.

Gellersen, B., Kempf, R., Telgmann, R. and DiMattia, G. E. (1994) ‘Nonpituitary human prolactin gene transcription is independent of Pit-1 and differentially controlled in lymphocytes and in endometrial stroma’, Molecular Endocrinology, 8(3), pp. 356–373.

Hiney, K. M., Nielsen, B. D., Rosenstein, D., Orth, M. W. and Marks, B. P. (2004) ‘High-intensity exercise of short duration alters bovine bone density and shape’, Journal of Animal Science, 82(6), pp. 1612–1620.

Keene, B. E., Knowlton, K. F., McGilliard, M. L., Lawrence, L. A., Nickols-Richardson, S. M., Wilson, J. H., Rutledge, A. M., McDowell, L. R. and Van Amburgh, M. E. (2004) ‘Measures of bone mineral content in mature dairy cows’, Journal of Dairy Science, 87(11), pp. 3816–3825.

Lee, Y. C., Raychaudhuri, S., Cui, J., de Vivo, I., Ding, B., Alfredsson, L., Padyukov, L., Costenbader, K. H., Seielstad, M., Graham, R. R., Klareskog, L., Gregersen, P. K., Plenge, R. M. and Karlson, E. W. (2009) ‘The PRL –1149 G/T polymorphism and rheumatoid arthritis susceptibility’, Arthritis and Rheumatism, 60(5), pp. 1250–1254.

Li, B. Y., Yang, Y. M., Liu, Y., Sun, J., Ye, Y., Liu, X. N., Liu, H. X., Sun, Z. Q., Li, M., Cui, J., Sun, D. J. and Gao, Y. H. (2017) ‘Prolactin rs1341239 T allele may have protective role against the brick tea type skeletal fluorosis’, PLoS ONE, 12(2), p. e0171011.

Li, J. J., Wang, B. Q., Fei, Q., Yang, Y. and Li, D. (2016) ‘Identification of candidate genes in osteoporosis by integrated microarray analysis’, Bone and Joint Research, 5(12), pp. 594–601.

Maetani, A., Itoh, M., Nishihara, K., Aoki, T., Ohtani, M., Shibano, K., Kayano, M. and Yamada, K. (2016) ‘Experimental assessment of bone mineral density using quantitative computed tomography in Holstein dairy cows’, Journal of Veterinary Medical Science, 78(7), pp. 1209–1211.

Mori, G., D’Amelio, P., Faccio, R. and Brunetti, G. (2013) ‘The interplay between the bone and the immune system’, Clinical and Developmental Immunology, 2013(720504), pp. 1–16.

Özbaş, H., Onrat, S. T. and Özdamar, K. (2012) ‘Genetic and environmental factors in human osteoporosis’, Molecular Biology Reports, 39(12), pp. 11289–11296.

Pellegrini, I., Lebrun, J. J., Ali, S. and Kelly, P. A. (1992) ‘Expression of prolactin and its receptor in human lymphoid cells’, Molecular Endocrinology, 6(7), pp. 1023–1031.

Pietschmann, P., Mechtcheriakova, D., Meshcheryakova, A., Föger-Samwald, U. and Ellinger, I. (2016) ‘Immunology of osteoporosis: A mini-review’, Gerontology, 62(2), pp. 128–137.

Ray, K. (2014) ‘Bone: The immune system takes control of bone homeostasis’, Nature Reviews Rheumatology, 10(7), pp. 382–382.

Rocha-Braz, M. G. M. and Ferraz-de-Souza, B. (2016) ‘Genetics of osteoporosis: Searching for candidate genes for bone fragility’, Archives of Endocrinology and Metabolism, 60(4), pp. 391–401.

Shupe, J. L., Butcher, J. E., Call, J. W., Olson, A. E. and Blake, J. T. (1988) ‘Clinical signs and bone changes associated with phosphorus deficiency in beef cattle’, American Journal of Veterinary Research, 49(9), pp. 1629–1636. PMID: 3223676

Somekawa, Y., Chiguchi, M., Ishibashi, T. and Aso, T. (2001) ‘Soy intake related to menopausal symptoms, serum lipids, and bone mineral density in postmenopausal Japanese women’, Obstetrics and Gynecology, 97(1), pp. 109–115.

Stevens, A., Ray, D., Alansari, A., Hajeer, A., Thomson, W., Donn, R., Ollier, W. E. R., Worthington, J. and Davis, J. R. E. (2001) ‘Characterization of a prolactin gene polymorphism and its associations with systemic lupus erythematosus’, Arthritis and Rheumatism, 44(10), pp. 2358–2366.<2358::AID-ART399>3.0.CO;2-K

Thomas, T. (2010) ‘Nouveaux acteurs du remodelage osseux, rôle du système immunitaire’, Bulletin de l’Academie Nationale de Medecine, 194(8), pp. 1493-4503. PMID: 22046712. Available at: [in French]

Wei, P., Liu, M., Chen, Y. and Chen, D.-C. (2012) ‘Systematic review of soy isoflavone supplements on osteoporosis in women’, Asian Pacific Journal of Tropical Medicine, 5(3), pp. 243–248.

Williams, S. N., Lawrence, L. A., McDowell, L. R., Wilkinson, N. S., Ferguson, P. W. and Warnick, A. C. (1991) ‘Criteria to evaluate bone mineralization in cattle: I. Effect of dietary phosphorus on chemical, physical, and mechanical properties’, Journal of Animal Science, 69(3), pp. 1232–1242.

Wythe, S. E., Nicolaidou, V. and Horwood, N. J. (2014) ‘Cells of the immune system orchestrate changes in bone cell function’, Calcified Tissue International, 94(1), pp. 98–111.

Zhang, X., Shu, X.-O., Li, H., Yang, G., Li, Q., Gao, Y.-T. and Zheng, W. (2005) ‘Prospective cohort study of soy food consumption and risk of bone fracture among postmenopausal women’, Archives of Internal Medicine, 165(16), pp. 1890–1895.

Zhang, Y., Liu, H., Zhang, C., Zhang, T., Zhang, B., Li, L., Chen, G., Fu, D. and Wang, K. (2015) ‘Endochondral ossification pathway genes and postmenopausal osteoporosis: Association and specific allele related serum bone sialoprotein levels in Han Chinese’, Scientific Reports, 5(1), p. 16783.

Zimin, A. V, Delcher, A. L., Florea, L., Kelley, D. R., Schatz, M. C., Puiu, D., Hanrahan, F., Pertea, G., Van Tassell, C. P., Sonstegard, T. S., Marçais, G., Roberts, M., Subramanian, P., Yorke, J. A. and Salzberg, S. L. (2009) ‘A whole-genome assembly of the domestic cow, Bos taurus’, Genome Biology, 10(4), p. R42.