Issue 4

Journal for Veterinary Medicine, Biotechnology and Biosafety

Volume 3, Issue 4, December 2017, Pages 28–30

ISSN 2411-3174 (print version) ISSN 2411-0388 (online version)

ESTIMATION OF MYCOBACTERIA RESISTANCE TO DISINFECTING PREPARATION

Danilova І. S., Zavgorodniy А. I.

National Scientific Center ‘Institute of Experimental and Clinical Veterinary Medicine’, Kharkiv, Ukraine, e-mail: andrii.i.zavgorodnii@gmail.com

Download PDF (print version)

Citation for print version: Danilova, І. S. and Zavgorodniy, А. I. (2017) ‘Estimation of Mycobacteria resistance to disinfecting preparation’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 3(4), pp. 28–30.

Download PDF (online version)

Citation for online version: Danilova, І. S. and Zavgorodniy, А. I. (2017) ‘Estimation of Mycobacteria resistance to disinfecting preparation’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 3(4), pp. 28–30. Available at: http://jvmbbs.kharkov.ua/archive/2017/volume3/issue4/oJVMBBS_2017034_028-030.pdf

Summary. Disinfection is the important measure in tuberculosis control systems. It allows preventing further environmental Mycobacteria distribution. The aim of work was to determine the bactericide properties of new disinfectant ‘Virosan Max’ on M. fortuitum depending on the solution concentration, time exposure, and bactericidal effect on M. bovis at different test objects. The study was carried out using classical microbiological techniques, reference, and field Mycobacteria strains. Bactericidal properties of disinfectant preparation ‘Virosan Max’ was studied on M. fortuitum depending its solution concentration and exposure. In addition, tuberculocidal effect on M. bovis at different test objects determined. Bactericidal properties of disinfectant preparation with the test culture of atypical Mycobacterium of type М. fortuitum determined a suspension method with application 1.0% and 3.0% water solution for displays 3, 5, and 24 hours. Tuberculocidal property disinfectant preparation with the test culture of М. bovis conducted on contamination objects of (glass, ceramic tile, and wood) tests with the biological loading and application of in 3.0% and 4.0% concentrations of solution for displays 5–24 hours. The bactericidal effect disinfectant preparation ‘Virosan Max’ was determined at presence height colonies Mycobacterium on a dense egg nourishing environment in control test tubes and absence of height initial cultures М. fortuitum, М. bovis in experience test tubes. The ‘Virosan Max’ disinfectant shows bacteriostatic properties only against atypical mycobacteria М. fortuitum in 1.0% concentrations under 3–24 hours of exposure, and in concentration 3.0% under 3–5 hours of exposure. Preparation ‘Virosan Max’ demonstrates the bactericide effect on tuberculosis agent of bovine species M. bovis in a 3.0% concentration with 24 hours’ exposure and 4.0% concentrations with 5–24 hours’ exposure.

Keywords: bactericidal properties, disinfectants, Mycobacterium bovis, Mycobacterium fortuitum, test objects

References:

Arkhipova, N. D. and Bessonova, N. M. (2007) ‘Action of disinfectants by tuberculosis’ [Deystvie dezinfitsiruyushchikh preparatov pri tuberkuleznoy infektsii], Bulletin of Altai State Agricultural University [Vestnik Altayskogo gosudarstvennogo agrarnogo universiteta], 5, pp. 58–61. Available at: http://elibrary.ru/item.asp?id=10293432. [in Russian]

Arzhakov, P. V. and Arzhakov, V. N. (2009) ‘The study of tuberculocidal properties of new disinfecting compositions’ [Izuchenie tuberkulotsidnykh svoystv novykh dezinfitsiruyushchikh kompozitsiy], Veterinary and Nutrition [Veterinariya i kormlenie], 4, pp. 21–22. Available at: http://elibrary.ru/item.asp?id=20391118. [in Russian]

Bezrukava, I. Iu., Nalyvaiko, L. I. and Nalyvaiko, I. M. (2008) ‘Disinfectants in veterinary practice’ [Dezinfikuiuchi zasoby u veterynarnii praktytsi], Poultry Breeding [Ptakhivnytstvo], 61, pp. 7–11. Available at: http://base.dnsgb.com.ua/files/journal/Ptahivnystvo/2008_61/index.files/2.pdf. [in Ukrainian]

Bublii, O. (2000) ‘Prevention is the basis of the welfare of dairy farms on tuberculosis’ [Profilaktyka — osnova blahopoluchchia molochnykh ferm shchodo tuberkulozu], Veterinary Medicine of Ukraine [Veterynarna medytsyna Ukrainy], 3, p. 18. [in Ukrainian].

Katoch, V. M. (2004) ‘Infections due to non-tuberculous mycobacteria (NTM)’, The Indian Journal of Medical Research, 120(4), pp. 290–304. Available at: http://www.ijmr.in/CurrentTopicView.aspx?year=Indian%20J%20Med%20Res%20120,%20October%202004,%20pp%20290-304%20(Review%20Article)$Review%20Article.

Shishkov, V.G. and Urban, V. P. (eds.) (1991) Tuberculosis of farm animals [Tuberkulez sel’skokhozyaystvennykh zhivotnykh]. Moscow: Agropromizdat. ISBN: 5100013818. [in Russian]

Skrypnyk, A. V. (2007) Molecular-genetic differentiation of mycobacteria isolated in Ukraine, and their phylogenetic interrelationships [Molekuliarno-henetychna dyferentsiatsiia mikobakterii, vydilenykh v Ukraini, ta yikh filohenetychni vzaiemozviazky]. The dissertation thesis for the scientific degree of the candidate of veterinary sciences. Kharkiv: National Scientific Center ‘Institute of Experimental and Clinical Veterinary Medicine’. Available at:  http://www.irbis-nbuv.gov.ua/cgi-bin/irbis_nbuv/cgiirbis_64.exe?C21COM=2&I21DBN=ARD&P21DBN=ARD&Z21ID=&IMAGE_FILE_DOWNLOAD=1&Image_file_name=DOC/2007/07savufv.zip. [in Ukrainian]

Zavhorodnii, A. I., Kalashnyk, N. V., Kochmarskyi, V. A., Busol, V. O., Palii, A. P., Tykhonov, P. M. and Horzheiev, V. M. (2007) Determination of bactericidal properties of disinfectants, disinfection and control of its quality in tuberculosis of farm animals: Methodological recommendations [Vyznachennia bakterytsydnykh vlastyvostei dezinfikuiuchykh zasobiv, provedennia dezinfektsii ta kontrol yii yakosti pry tuberkulozi silskohospodarskykh tvaryn: metodychni rekomendatsii]. Kharkiv. [in Ukrainian]