Issue 3
Journal for Veterinary Medicine, Biotechnology and Biosafety
Volume
4, Issue 3, September 2018, Pages 28–30
ISSN 2411-3174 (print version) ISSN 2411-0388
(online version)
VIROLOGICAL MONITORING OF
ESPECIALLY DANGEROUS AVIAN PATHOGENS IN SOUTHERN UKRAINE IN 2017 DURING
AFTER-EPIZOOTIC PERIOD
Pishchanskyi O. V. 2,
Stegniy B. T. 1, Tkachenko S. V. 1,
Rula O. M. 1, Muzyka D. V. 1
1 National Scientific Center ‘Institute of Experimental and Clinical
Veterinary Medicine’, Kharkiv, Ukraine, e-mail: dmuzyka77@gmail.com
2 State Scientific and Research Institute of
Laboratory Diagnostics and Veterinary and Sanitary Expertise, Kyiv, Ukraine
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PDF (print version)
Citation for print version: Pishchanskyi, O. V.,
Stegniy, B. T., Tkachenko, S. V., Rula, O. M. and
Muzyka, D. V. (2018) ‘Virological monitoring of especially
dangerous avian pathogens in Southern Ukraine in 2017 during after-epizootic
period’, Journal for
Veterinary Medicine, Biotechnology and Biosafety, 4(3), pp. 28–30.
Download
PDF (online version)
Citation for online version: Pishchanskyi, O. V.,
Stegniy, B. T., Tkachenko, S. V., Rula, O. M. and
Muzyka, D. V. (2018) ‘Virological monitoring
of especially dangerous avian pathogens in Southern Ukraine in 2017 during
after-epizootic period’, Journal
for Veterinary Medicine, Biotechnology and Biosafety.
[Online] 4(3), pp. 28–30. Available at: http://jvmbbs.kharkov.ua/archive/2018/volume4/issue3/oJVMBBS_2018043_028-030.pdf
Summary. Constant monitoring of the circulation of
particularly dangerous avian diseases’ causative agents (influenza and
Newcastle disease) in natural reservoir is an important component of global
surveillance and control of these diseases. The main objective of the research
is to control the circulation of influenza viruses, Newcastle disease and other
avulaviruses in poultry in Ukraine, isolation of influenza virus isolates from
H5 and H7 subtypes for further study. Classical virological methods of
viruses’ isolation using chicken embryos, serological tests using
referent blood sera as well as identification of viruses via real-time PCR were used for research. Ten influenza viruses of different
subtypes (including one influenza virus of H5 subtype and two influenza viruses
of H7 subtype), as well as various avulaviruses of different serotypes
(including AvV-1 — Newcastle disease virus) were isolated from
1,619 wild waterfowl and water-related birds of 28 species during
autumn migration in 2017 in Kherson, Odesa, and Zaporizhia regions. All the
viruses are isolated from wild ducks, geese and gulls. Hemagglutinating viruses
from wild birds of such ecological groups as waders, herons and land birds have
not been isolated. The level of infection of wild waterfowl (wild ducks) during
the autumn migration in 2017 was 1.44–1.58%, and waterfowl (gulls) —
0.42%. The data we obtained is very important for predicting the epizootic
situation in Ukraine, understanding the ecological features of low-pathogenic
variants of viruses, which cause particularly dangerous avian viral diseases.
In addition, conducting regular virological monitoring in a natural reservoir
allows us to receive new strains for improvement of laboratory diagnostics.
Keywords: H5 and H7 subtypes of avian influenza,
Newcastle disease, wild birds, surveillance, Ukraine
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