Journal for Veterinary Medicine, Biotechnology and Biosafety
Volume 5, Issue 1, March 2019, Pages 10–12
ISSN 2411-3174 (print version) ISSN 2411-0388 (online version)
DETERMINATION OF THE VITAMIN-MINERAL PREPARATION ‘ENERGOLIT’ STABILITY FOR THE TREATMENT OF METABOLIC DISORDERS IN ANIMALS
Sachuk R. M.
Research Epizootology Station of the Institute of Veterinary Medicine of the National Academy of Agrarian Sciences of Ukraine, Rivne, Ukraine, e-mail: email@example.com
Download PDF (print version)
Citation for print version: Sachuk, R. M. (2019) ‘Determination of the vitamin-mineral preparation ‘Energolit’ stability for the treatment of metabolic disorders in animals’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 5(1), pp. 10–12.
Download PDF (online version)
Citation for online version: Sachuk, R. M. (2019) ‘Determination of the vitamin-mineral preparation ‘Energolit’ stability for the treatment of metabolic disorders in animals’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 5(1), pp. 10–12. DOI: 10.36016/JVMBBS-2019-5-1-2.
Summary. The aim of experiments was the study the shelf life of the injectable preparation ‘Energolit’ when stored in vials of light glass. Studies were carried out at a temperature within from +10 to +25 °С and relative humidity (60 ± 5%). Experiments were carried out in 3, 6, 9, 12, 18, 24 months, and one day after the expiration date after the puncture of the stopper with a sterile injection needle. The control was the newly prepared preparation. We checked organoleptic parameters, pH, microbial contamination to assess the quality of the ‘Energolit’. In addition, we determined the quantitative content of vitamins B1, B2, B3, B5, B6, and B12 by methods described in the ‘State Pharmacopoeia of Ukraine’. Three tested batches of the preparation ‘Energolit’ showed constant composition throughout all period of study during two years of storage, as well as one day after the puncture of the stopper with a sterile injection needle in the primary packaging. Experimental samples of the preparation were within possible variation during the tests on changes of pH, identity and content of the vitamins B1, B2, B3, B5, B6, and B12. Microbial contamination (Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa) was not detected. Fungi contamination were not detected during the experiment. Fungi contamination was less than 10 CFU/ml one day after the puncture. The optimal storage terms of the preparation has been determined. The findings indicated, that the shelf life of the preparation ‘Energolit’ is 2 years. The pH and mass fraction of the vitamins B group were unchanged or were within the limits of possible variation in the tested samples during the experiment. Microbial contamination was not detected.
Keywords: B vitamins, stability, shelf life, ‘Energolit’
Belikov, V. G. (1985) Pharmaceutical chemistry [Farmatsevticheskaya khimiya]. Moscow: Vysshaya shkola [in Russian]
Kotsiumbas, I. Ya., Malyk, O. H., Patereha, I. P., Tishyn, O. L. and Kosenko, Yu. M. (2006) Preclinical studies of veterinary drugs [Doklinichni doslidzhennia veterynarnykh likarskykh zasobiv]. Lviv: Triada plus. ISBN 9667596648. [in Ukrainian]
Rokitskiy, P. F. (1973) Biological statistics [Biologicheskaya statistika]. 3rd ed. Minsk: Vysheyshaya shkola. [in Russian]
Sachuk, R. M. (2018) ‘Investigation of acute toxicity and local irritation of the vitamin-mineral preparation «Energolit»’ [Vyznachennia parametriv hostroi toksychnosti ta mistsevo-podrazniuiuchoi dii vitaminno-mineralnoho preparatu «Enerholit»], Veterinary Biotechnology [Veterynarna biotekhnolohiia], 33, pp. 109–116. Available at: http://nbuv.gov.ua/UJRN/vbtb_2018_33_16. [in Ukrainian]
Tykhonov, O. I. and Yarnykh T. H. (2016) Pharmacy drug technology [Aptechna tekhnolohiia likiv]. 4th ed. Vinnytsia: Nova knyha. ISBN 9789663825571. [in Ukrainian]
USPCQM [Ukrainian Scientific Pharmacopoeial Center for Quality of Medicines] (2014–2018) State Pharmacopoeia of Ukraine [Derzhavna Farmakopeia Ukrainy]. 2nd ed. Kharkiv: USPCQM [in Ukrainian]