Journal for Veterinary Medicine, Biotechnology and Biosafety

Volume 5, Issue 1, March 2019, Pages 20–26

ISSN 2411-3174 (print version) ISSN 2411-0388 (online version)

DOI: 10.36016/JVMBBS-2019-5-1-5


Zezekalo V. K. 1, Peredera S. B. 1, Buslyk T. V. 2, Pochernyaev K. F. 2, Shcherbakova N. S. 1

1 Poltava State Agrarian Academy, Poltava, Ukraine, e-mail:

2 Institute of Pig Breeding and Agroindustrial Production of the National Academy of Agrarian Sciences, Poltava, Ukraine

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Citation for print version: Zezekalo, V. K., Peredera, S. B., Buslyk, T. V., Pochernyaev, K. F. and Shcherbakova, N. S. (2019) ‘PCR test systems for the Clavichlamydia salmonicola and Piscichlamydia salmonis detection in fish’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 5(1), pp. 20–26.

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Citation for online version: Zezekalo, V. K., Peredera, S. B., Buslyk, T. V., Pochernyaev, K. F. and Shcherbakova, N. S. (2019) ‘PCR test systems for the Clavichlamydia salmonicola and Piscichlamydia salmonis detection in fish’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 5(1), pp. 20–26. DOI: 10.36016/JVMBBS-2019-5-1-5.

Summary. The aim of our work was to develop PCR test systems for the identification and differentiation of the Piscichlamydia salmonis and Clavochlamydia salmonicola, species, that are known epitheliocystis infection agents of gill and fish skin diseases, characterized by the presence of specific ‘inclusions’ in the epithelial cells of the gills. To date, the diseases of fish associated with chlamydial infections have been detected in more than 90 species of freshwater and marine fish worldwide. For now, there is no available information on the prevalence of Piscichlamydia salmonis and Clavochlamydia salmonicola, which can cause epitheliocystis of commercially important aquaculture species in Ukraine. Identification of these pathogens is possible only using molecular genetic methods. As a result of our research, we got PCR tests for the identification and species differentiation of Piscichlamydia salmonis and Clavochlamydia salmonicola. The use of diagnostics for the identification of Piscichlamydia salmonis and Clavochlamydia salmonicola makes chlamydial infections monitoring among various fish species possible and it will increase the economic efficiency of fish farms.

Keywords: Piscichlamydia salmonis, Clavochlamydia salmonicola, chlamydial infections in fish, Ukraine, PCR, Atlantic salmon, Salmo salar, brown trout, Salmo trutta


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