Journal for Veterinary Medicine, Biotechnology and Biosafety

Volume 5, Issue 2, June 2019, Pages 26–30

ISSN 2411-3174 (print version) ISSN 2411-0388 (online version)


Bondarchuk A. O. 1, Paliy A. P. 2, Blazheyevskiy M. Ye. 3

1 Kharkiv State Zooveterinary Academy, Kharkiv, Ukraine, е-mail:

2 National Scientific Center ‘Institute of Experimental and Clinical Veterinary Medicine’, Kharkiv, Ukraine, е-mail:

3 National University of Pharmacy, Kharkiv, Ukraine, e-mail:

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Citation for print version: Bondarchuk, A. O., Paliy, A. P. and Blazheyevskiy, M. Ye. (2019) ‘Determination of acute toxicity of the ‘Bondarmin’ disinfectant’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 5(2), pp. 26–30.

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Citation for online version: Bondarchuk, A. O., Paliy, A. P. and Blazheyevskiy, M. Ye. (2019) ‘Determination of acute toxicity of the ‘Bondarmin’ disinfectant’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 5(2), pp. 26–30. DOI: 10.36016/JVMBBS-2019-5-2-5.

Summary. In the article the results of the study of toxic effect of the designed disinfectant (active ingredient potassium peroxomonosulfate) on laboratory animals (mice) are presented. For the recent years a variety of scientific works both by domestic and by foreign scientists has been devoted to the study of different disinfectants’ toxicity. However today there is a number of issues that require more detailed studying and scientific justification. Among them the problem of toxic effects of disinfectants on the animal organism occupies a special place. The aim of our work was to study the toxic effect on the laboratory animals and to assess the acute toxicity (LD50) of the designed ‘Bondarmin’ disinfectant. Tests were carried out at the Laboratory of pharmacology and toxicology of the National University of Pharmacy (Kharkiv) and on the base of Educational and scientific laboratory of genetic and molecular research methods named after P. I. Verbitskiy in the Kharkiv State Zooveterinary Academy. Acute toxicity assessment (LD50) was carried out with intragastrointestinal administration of the designed drug to laboratory animals (mice). Changes in the internal organs of animals that were removed from the experiment for humane reasons and those who died after the experiment were detected by macroscopic examination. The lethality of laboratory animals after the intragastric administration of disinfectant was determined by the Prozorovskiy method The dynamic of changes in body weight of mice after the administration of disinfectant in high doses (from 1,500 to 3,500 mg/kg) was found out. The influence of the disinfectant on the mass coefficients of the internal organs of male mice after intragastric administration was evaluated. Toxic effect of the designed disinfectant ‘Bondarmin’, when using intragastric method of administration to laboratory animals (mice), according to the age and sexual index (LD50 = 2,702.40 ± 156.32 mg/kg), was established. Disinfectant ‘Bondarmin’ refers to IV toxicity class (low toxic substances).

Keywords: disinfectant, laboratory animals, toxic effect, lethal dose


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