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Journal for Veterinary Medicine, Biotechnology and Biosafety

Volume 5, Issue 3, September 2019, Pages 22–26

ISSN 2411-3174 (print version) ISSN 2411-0388 (online version)

DETERMINATION OF TOXICITY INDICATORS AND ASSESSMENT OF THE SENSIBILIZING ACTION OF THE PREPARATION FOR THE EXTERNAL USE ‘OINTMENT FOR WOUNDS’

Sachuk R. M.

Research Epizootology Station of the Institute of Veterinary Medicine of the National Academy of Agrarian Sciences of Ukraine, Rivne, Ukraine, е-mail: sachuk.08@ukr.net

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Citation for print version: Sachuk, R. M. (2019) ‘Determination of toxicity indicators and assessment of the sensibilizing action of the preparation for the external use ‘Ointment for wounds’’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 5(3), pp. 22–26.

Download PDF (online version)

Citation for online version: Sachuk, R. M. (2019) ‘Determination of toxicity indicators and assessment of the sensibilizing action of the preparation for the external use ‘Ointment for wounds’’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 5(3), pp. 22–26. DOI: 10.36016/JVMBBS-2019-5-3-5.

Summary. The purpose of the work is a determination of toxicity and sensitizing effects of a new external preparation ‘Ointment for wounds’, based on the essential oils of Siberian pine, eucalypt, tea-tree, cedar, clove, and oil solution of chlorophyllite. The experimental study was performed on rats weighing 160–190 g, 2–3 months of age and mice weighing 18–21 g, 3 months of age. At the stages of preclinical study, it was determined acute and subacute effects of the drug, when administrated on the skin and directly into the stomach of experimental animals. The skin-irritant effect of the phytopreparation was investigated when applied to the skin and buccal mucous membrane. The sensitizing effect of the ointment was evaluated by reproducing local reactions. The acute toxicity assessment was performed on the survival rate of laboratory animals after oral administration of the preparation at doses from 5,000 to 25,000 mg/kg of body weight, with 5,000 units increments. Subacute toxicity in the experiment was evaluated by the dynamics of morphological and biochemical parameters of blood and the study of the coefficients of laboratory animals’ internal organs mass. It was found that the introduction of the drug ‘Ointment for wounds’ in the stomach did not cause significant changes in the behavior of rats, all animals remained alive. Studies have shown the absence of skin-irritant effect of the preparation, as well as the absence of irritant effect on the buccal mucous membrane. Even in a long-term experiment to study subacute toxicity when applied to the skin, no toxic effects of the preparation on the basis of essential oils and oil solution chlorophyllite of were found. The index sensitizing effect of the preparation was less than one, indicating the absence of sensitizing effect. According to the classification of substances by toxicity and danger (requirements of SOU 85.2-37-736:2011 and GOST 12.1.007-76) belong to hazard class IV. In general, ‘Ointment for wounds’ does not have a toxic effect on the functions of vital organs, and at repeated administration is almost harmless

Keywords: essential oil, chlorophyllite, toxity, rats, mice, skin-irritant effect, sensitizing action, morphological composition of blood, transaminase activity

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