Journal for Veterinary Medicine, Biotechnology and Biosafety
Volume 5, Issue 4, December 2019, Pages 22–26
ISSN 2411-3174 (print version) ISSN 2411-0388 (online version)
EMBRYOTOXIC AND TERATOGENIC EFFECTS OF ‘VITOSEPT’ ON WHITE RATS
Soltys M. P., Rudyk H. V., Gunchak V. M., Gutyi B. V.
Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies, Lviv, Ukraine, е-mail: email@example.com
Download PDF (print version)
Citation for print version: Soltys, M. P., Rudyk, H. V., Gunchak, V. M. and Gutyi, B. V. (2019) ‘Embryotoxic and teratogenic effects of ‘Vitosept’ on white rats’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 5(4), pp. 22–26.
Download PDF (online version)
Citation for online version: Soltys, M. P., Rudyk, H. V., Gunchak, V. M. and Gutyi, B. V. (2019) ‘Embryotoxic and teratogenic effects of ‘Vitosept’ on white rats’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 5(4), pp. 22–26. DOI: 10.36016/JVMBBS-2019-5-4-6.
Summary. The effect of ‘Vitosept’ drug, based on sodium hypochlorite solution with high purity, obtained in a specially developed membraneless flow electrolyzer, on the embryotoxic and teratogenic properties in rats was investigated. To determine the embryotoxic effect of ‘Vitosept’ on the development of white rats offspring of the 1st generation, control and three experimental groups (G1, G2, G3) were formed from pregnant females. The females of the control group with a blunt probe were injected daily for 30 days with 5 ml of isotonic sodium chloride solution, and experimental ones with 5 ml of ‘Vitosept’ drug with different concentration of high purity sodium hypochlorite: Group I (G1) — 50 mg/l; Group II (G2) — 100 mg/l; Group III (G3) — 500 mg/l. The animals were observed. During the observation the condition and behavior of the females, the dynamics of body weight change, duration of pregnancy, and the course of birth were monitored. The results of the experiment were recorded after the slaughter of pregnant females (20th day of pregnancy) and in the postnatal period of development of the offspring. Studies have shown that the use of different concentrations of the drug ‘Vitosept’ in rats for 30 days before and during pregnancy has no embryotoxic and teratogenic effects. According to the indicators of the total, pre- and postimplantation lethality of embryos, there were no reliable changes in the structure and morphometry of internal organs and tissues in 20-day-old fetuses, and their development corresponded to the terms of pregnancy. There was no significant difference between the fertility of female rats in the test and control groups. The average number of fetuses per female was within 9 animals. The rats obtained from the females of the experimental groups were viable and did not lag behind in growth and development compared with the control animals, which generally characterizes the studied drug ‘Vitosept’ as non-toxic, lacking embryotoxic and teratogenic action
Keywords: sodium hypochlorite, white rats, embryotoxic effect, teratogenic action
Baryliak, I. P., Neumerzhytska, L. V., Byshovets, T. F. and Danylenko, B. C. (2001) ‘Study of the gonadotoxic effects of new drugs and their influence on the animal reproductive function’ [Vyvchennia honadotoksychnoi dii novykh likarskykh zasobiv ta yikh vplyvu na reproduktyvnu funktsiiu tvaryn], in Stefanov, O. V. (ed.) Preclinical Studies of Drugs [Doklinichni doslidzhennia likarskykh zasobiv]. Kyiv: Avitsenna, pp. 139–152. [in Ukrainian]
Benz, R. D. and Beltz, P. A. (1980) ‘Cytogenetic toxicologic testing with dogs’, Environmental Mutagenesis, 2(2), pp. 312–313. doi: https://doi.org/10.1002/em.2860020214
CE (The Council of Europe). (1986) European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes. (European Treaty Series, No. 123). Strasbourg: The Council of Europe. Available at: https://conventions.coe.int/treaty/en/treaties/html/123.htm
CEC (The Council of the European Communities). (1986) ‘Council Directive 86/609/EEC of 24 November 1986 on the approximation of laws, regulations and administrative provisions of the Member States regarding the protection of animals used for experimental and other scientific purposes’, The Official Journal of the European Communities, L 358, pp. 1–28. Available at: http://data.europa.eu/eli/dir/1986/609/oj
Durnev, A. D. and Seredenin, S. B. (1998) Mutagens. Screening and Pharmacological Prevention of Effects [Mutageny. Skrining i farmakologicheskaya profilaktika vozdeystviy]. Moscow: Meditsina. ISBN 5225044298. [in Russian]
Dyban, A. P. (ed.) (1986) Methodological Guidelines for the Study of the Embryotoxic Action of Pharmacological Substances and the Effect on Reproductive Function [Metodieskie ukazaniya po izucheniyu embriotoksicheskogo deystviya farmakologicheskikh veshchestv i vliyaniya na reproduktivnoyu funktsiyu]. Moscow. [in Russian]
Hayashi, M., Sofuni, T. and Ishidate, M. (1984) ‘Mechanism of micronuclei formation in mouse bone marrow’, Mutation Research, 130(5), p. 364. doi: https://doi.org/10.1016/0165-1161(84)90031-1
Il’inskikh, N. N., Medvedev, M. A., Bessudnova, S. S. and Il’inskikh, I. N. (1990) Mutagenesis at Different Functional States of an Organism [Mutagenez pri razlichnykh funktsional'nykh sostoyaniyakh organizma]. Tomsk: Tomsk University Press. ISBN 575110972. [in Russian]
Kotsiumbas, I. Ya. and Kotsiumbas, G. I. (2000) ‘Features of morphological research in the study of new drugs’ toxicity’ [Osoblyvosti morfolohichnykh doslidzhen pry vyvcheni toksychnosti novykh likarskykh zasobiv], Bulletin of the Bila Tserkva State Agrarian University [Visnyk Bilotserkivskoho derzhavnoho ahrarnoho universytetu], 13(2), pp. 79–84. [in Ukrainian]
Kotsiumbas, I. Ya. and Velichenko, O. B. (eds.) (2009) Prospects for the Use of Hypochlorites in Veterinary Medicine [Perspektyvy zastosuvannia hipokhlorytiv u veterynarnii medytsyni]. Lviv: Afisha. ISBN 9789663251226. [in Ukrainian]
Kotsiumbas, I. Ya., Malyk, O. H., Patereha, I. P., Tishyn, O. L. and Kosenko, Yu. M. (2006) Preclinical studies of veterinary drugs [Doklinichni doslidzhennia veterynarnykh likarskykh zasobiv]. Lviv: Triada plus. ISBN 9667596648. [in Ukrainian]