Journal for Veterinary Medicine, Biotechnology and Biosafety

Volume 6, Issue 2, February 2020, Pages 16–19

ISSN 2411-3174 (print version) ISSN 2411-0388 (online version)

STUDY OF BIOLOGICAL PROPERTIES OF FIELD ISOLATES OF CATTLE MINOR INFECTIONS AGENTS ON HOMOLOGICAL CELL CULTURES

Gorbatenko S. K. 1, Solodiankin O. S. 1, Lymanska O. Yu. 1, Rudova N. G. 1, Kulyk I. O. 1, Kornieikov O. M. 1, Stegniy M. Yu. 1, Kuznetsova O. V. 1, Dunaeva O. V. 2, Gorbatenko V. P. 3

1 National Scientific Center ‘Institute of Experimental and Clinical Veterinary Medicine’, Kharkiv, Ukraine, e-mail: st.gorbatenko@gmail.com

2 Kharkiv National Medical University, Kharkiv, Ukraine

3 Kharkiv State Zooveterinary Academy, Kharkiv, Ukraine

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Citation for print version: Gorbatenko, S. K., Solodiankin, O. S., Lymanska, O. Yu., Rudova, N. G., Kulyk, I. O., Kornieikov, O. M., Stegniy, M. Yu., Kuznetsova, O. V., Dunaeva, O. V. and Gorbatenko, V. P. (2020) ‘Study of biological properties of field isolates of cattle minor infections agents on homological cell cultures’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 6(2), pp. 16–19.

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Citation for online version: Gorbatenko, S. K., Solodiankin, O. S., Lymanska, O. Yu., Rudova, N. G., Kulyk, I. O., Kornieikov, O. M., Stegniy, M. Yu., Kuznetsova, O. V., Dunaeva, O. V. and Gorbatenko, V. P. (2020) ‘Study of biological properties of field isolates of cattle minor infections agents on homological cell cultures’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 6(2), pp. 16–19. DOI: 10.36016/JVMBBS-2020-6-2-3.

Summary. Biological properties of field isolates of bovine immunodeficiency virus and bovine foamy virus on homological cell cultures (fetal bovine lung and bovine coronary artery endothelial cells) were investigated. Pathogens of bovine slow infections, namely bovine immunodeficiency virus and bovine foamy virus, are able to integrate into cell cultures of homologous to cattle type, which is confirmed by the results of PCR. There has been determined the presence of genetic material of pathogens of bovine immunodeficiency (BIV) and spumavirus infection (BFV) in the cultivation of lymphocytes of field isolates in the culture of bovine coronary artery endothelial cells (BCAEC) at the level of 5th passage, and in the cell culture of fetal bovine lung (FBL) — at the level of 10th passage. In the process of integration of pathogens of immunodeficiency and spumavirus infection of cattle in continuous cell cultures FBL and BCAEC, morphological changes in the state of the monolayer by the principle of syncytiation and vacuolation are observed

Keywords: bovine immunodeficiency virus, bovine foamy virus, cell cultures, PCR, cattle

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