Journal for Veterinary Medicine, Biotechnology and Biosafety
Volume
6, Issue 2, February 2020, Pages 20–26
ISSN 2411-3174 (print version) ISSN 2411-0388
(online version)
DETERMINATION OF ACUTE
TOXICITY PARAMETERS OF THE DRUG ‘MEGASTOP FOR DOGS’ ON WHITE RATS
AND MICE
Orobchenko
O. L. 1, Romanko M. Ye. 1, Yaroshenko M.
O. 1, Gerilovych I. O. 1, Zhukova N. A. 2,
Sapko S. A. 2, Maslyuk A. V. 3
1 National
Scientific Center ‘Institute of Experimental and Clinical Veterinary Medicine’,
Kharkiv, Ukraine, e-mail: toxi-lab@ukr.net
2 Research and
Production Enterprise ‘Suziria’ LLC, Kharkiv, Ukraine, e-mail: zhukova.n@priroda.ua
3 State
Scientific and Research Institute of Laboratory Diagnostics and Veterinary and
Sanitary Expertise, Kyiv, Ukraine, е-mail: maslychok@ukr.net
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PDF (print version)
Citation for print version: Orobchenko, O. L., Romanko, M. Ye.,
Yaroshenko, M. O., Gerilovych, I. O., Zhukova, N. A., Sapko, S. A. and Maslyuk,
A. V. (2020) ‘Determination of acute toxicity parameters of the drug
‘MEGASTOP for dogs’ on white rats and mice’, Journal for Veterinary Medicine, Biotechnology and
Biosafety, 6(2), pp. 20–26.
Download
PDF (online version)
Citation for online version: Orobchenko, O. L., Romanko, M. Ye.,
Yaroshenko, M. O., Gerilovych, I. O., Zhukova, N. A., Sapko, S. A. and Maslyuk,
A. V. (2020) ‘Determination of acute toxicity parameters of the drug
‘MEGASTOP for dogs’ on white rats and mice’, Journal for Veterinary Medicine, Biotechnology and
Biosafety. [Online] 6(2), pp. 20–26. DOI: 10.36016/JVMBBS-2020-6-2-4.
Summary. The
experiments were performed on 58 males of nonlinear white rats
3–4 months old and weighing 180–200 g and 64 females
of nonlinear white mice 2.5–3 months old and weighing
18–22 g. In the main experiment on rats, six experimental groups
were formed, the animals of which were injected intragastrically with the drug
‘MEGASTOP for dogs’ (by absolute weight) in doses of 1,000.0,
2,000.0, 3,000.0, 4,000.0, 5,000.0, and 6,000.0 mg/kg body weight; in the
main experiment on mice, seven experimental groups were formed, the animals of
which were administered the drug in doses of 100.0, 500.0, 1,000.0, 1,500.0,
2,000.0, 2,500.0, and 3,000.0 mg/kg body weight. Control rats and mice
were injected with 2.0 cm3 and 0.2 cm3 of
polyethylene glycol-400, respectively. Clinical symptoms of poisoning with the
drug ‘MEGASTOP for dogs’ of white rats (at doses of
2,000.0–6,000.0 mg/kg body weight) and mice (at doses of
1,000.0–3,000.0 mg/kg body weight) were refusals of food and water,
loss of coordination, sitting in one place, a dose-dependent increase in
depression with subsequent complete depression, lack of response to external
stimuli and death on the first or fourth day after administration. During
autopsy in rats and mice that died as a result of poisoning with the drug ‘MEGASTOP
for dogs’, we recorded pallor of the mucous membranes of the mouth,
trachea, pharynx, and esophagus; increase in heart volume, atrial blood supply;
pulmonary hyperemia; uncoagulated blood; increase in liver volume, dark cherry
color, flabby consistency; catarrhal inflammation of the mucous membrane of the
small intestine. According to the results of determining the parameters of
acute toxicity of the drug ‘MEGASTOP for dogs’ in the case of a
single intragastric injection, LD50 for male rats is 3,384.98 ± 444.94 mg/kg,
and for female mice — 2,025.88 ± 279.46 mg/kg
body weight, which allows to classify it to class IV by the
toxicity — low-toxic substances (LD50 —
501–5,000 mg/kg) and by the degree of danger to
class III— moderately dangerous substances (LD50 —
151–5,000 mg/kg)
Keywords: acute toxicity, male rats, female mice, dose,
intragastric administration, ‘MEGASTOP for dogs’
References:
CE (The Council of Europe). (1986) European Convention for the Protection of
Vertebrate Animals Used for Experimental and Other Scientific Purposes. (European Treaty Series, No. 123).
Strasbourg: The Council of Europe. Available at: https://conventions.coe.int/treaty/en/treaties/html/123.htm
CEC (The Council of the European Communities). (1986) ‘Council
Directive 86/609/EEC of 24 November 1986 on the approximation of laws,
regulations and administrative provisions of the Member States regarding the
protection of animals used for experimental and other scientific
purposes’, The Official Journal of
the European Communities, L 358, pp. 1–28. Available at: http://data.europa.eu/eli/dir/1986/609/oj
Diakou, A., Di Cesare, A., Morelli, S.,
Colombo, M., Halos, L., Simonato, G., Tamvakis, A.,
Beugnet, F., Paoletti, B. and Traversa, D. (2019)
‘Endoparasites and vector-borne pathogens in dogs from Greek islands:
Pathogen distribution and zoonotic implications’, PLOS Neglected
Tropical Diseases, 13(5), p. e0007003. doi: https://doi.org/10.1371/journal.pntd.0007003
Elbert, A., Nauen, R. and Leicht, W. (1998)
‘Imidacloprid, a novel chloronicotinyl insecticide: Biological activity
and agricultural importance’, in Ishaaya, I. and Degheele, D.
(eds.) Insecticides with Novel Modes of Action. Berlin, Heidelberg:
Springer, pp. 50–73. doi: https://doi.org/10.1007/978-3-662-03565-8_4
Gomez, S. A. and Picado, A. (2017) ‘Systemic
insecticides used in dogs: potential candidates for phlebotomine vector
control?’, Tropical Medicine and International Health, 22(6), pp. 755–764.
doi: https://doi.org/10.1111/tmi.12870
Gosstandart (The USSR State Committee of
Standards) (1976) GOST 12.1.007-76. Occupational Safety Standards
System. Noxious Substances. Classification and General Safety Requirements
[Sistema standartov bezopasnosti truda. Vrednye veshchestva. Klassifikatsiya i
obshchie trebovaniya bezopasnosti]. Moscow: Izdatel’stvo standartov.
[in Russian]
Hasib, Y., Kabir, H., Barua, S., Akter, S. and
Chowdhury, S. (2020) ‘Frequency and prevalence of clinical
conditions and therapeutic drugs used in dog and cat at Teaching Veterinary
Hospital, Chattogram Veterinary and Animal Sciences University’, Journal
of Advanced Veterinary and Animal Research, 7(1), p. 156–163. doi:
https://doi.org/10.5455/javar.2020.g405
Havryk, K. A. (2015) ‘Influence of demodicosis and
otodectosis pathogens on blood serum biochemical parameters of sick dogs’
[Vplyv zbudnykiv demodekozu ta otodektozu na biokhimichni pokaznyky syrovatky
krovi khvorykh sobak], Scientific
Bulletin of Veterinary Medicine [Naukovyi visnyk veterynarnoi medytsyny],
1, pp. 68–71. Available at: http://nbuv.gov.ua/UJRN/nvvm_2015_1_17.
[in Ukrainian]
Karkishchenko, N. N. and Grachev, S. V. (eds.)
(2010) The Guide to Laboratory Animals
and Alternative Models in Biomedical Researches [Rukovodstvo po laboratornym
zhivotnym i al’ternativnym modelyam v biomeditsinskikh tekhnologiyakh].
Moscow: Profil’. ISBN 9785903950102. [in Russian]
Klafke, G. M., Moreno, H. C.,
Tidwell, J. P., Miller, R. J., Thomas, D. B.,
Feria-Arroyo, T. P. and
Pérez de León, A. A. (2020) ‘Partial
characterization of the voltage-gated sodium channel gene and molecular detection
of permethrin resistance in Rhipicephalus
annulatus (Say, 1821)’, Ticks and Tick-Borne Diseases, 11(3),
p. 101368. doi: https://doi.org/10.1016/j.ttbdis.2019.101368
Kotsiumbas, I. Ya., Malyk, O. H., Patereha, I. P.,
Tishyn, O. L., Kosenko, Yu. M., Chura, D. O.,
Kotsiumbas, H. I., Piatnychko, O. M.,
Brezvyn, O. M., Zasadna, Z. S. and
Chaikovska, O. I. (2006) Preclinical
Studies of Veterinary Drugs [Doklinichni doslidzhennia veterynarnykh likarskykh
zasobiv]. Lviv: Triada plus. ISBN 9667596648. [in Ukrainian]
Kumar, R., Klafke, G. M. and Miller, R. J.
(2020) ‘Voltage-gated sodium channel gene mutations and pyrethroid
resistance in Rhipicephalus microplus’,
Ticks and Tick-Borne Diseases, 11(3), p. 101404. doi: https://doi.org/10.1016/j.ttbdis.2020.101404
Laing, R., Gillan, V. and Devaney, E. (2017)
‘Ivermectin — old drug, new tricks?’, Trends in
Parasitology, 33(6), pp. 463–472. doi: https://doi.org/10.1016/j.pt.2017.02.004
Prozorovskiy, V. B. (2007) ‘Statistic processing of data
of pharmacological investigations’ [Statisticheskaya obrabotka
rezul’tatov farmakologicheskikh issledovaniy], Psychopharmacology and Biological Narcology [Psikhofarmakologiya i
biologicheskaya narkologiya], 7(3–4), pp. 2090–2120.
Available at: https://www.elibrary.ru/item.asp?id=11691694.
[in Russian]
Sheele, J. M. and Ridge, G. E. (2016)
‘Toxicity and potential utility of ivermectin and moxidectin as
xenointoxicants against the common bed bug, Cimex
lectularius L.’, Parasitology Research, 115(8),
pp. 3071–3081. doi: https://doi.org/10.1007/s00436-016-5062-x
Tucker, N. S. G., Kaufman, P. E. and
Weeks, E. N. I. (2019) ‘Identification of permethrin and
etofenprox cross‐tolerance in Rhipicephalus sanguineus sensu lato (Acari:
Ixodidae)’, Pest Management Science, 75(10),
pp. 2794–2801. doi: https://doi.org/10.1002/ps.5391
Van Emden, H. F. (2019) Statistics for Terrified Biologists. 2nd ed.
Hoboken, NJ: John Wiley & Sons. ISBN 9781119563679
Zapadnyuk, I. P., Zapadnyuk, V. I.,
Zakhariya, E. A. and Zapadnyuk, B. V. (1983) Laboratory animals. Breeding, Keeping, Use
in the Experiment [Laboratornye zhivotnye. Razvedenie, soderzhanie,
ispol’zovanie v eksperimente]. 3rd ed. Kiev: Vishcha
shkola. [in Russian]
Zharov, A. V., Ivanov, I. V. and
Strel’nikov, A. P. (2003) Autopsy
and Pathomorphological Diagnosis of Animal Diseases [Vskrytie i
patomorfologicheskaya diagnostika bolezney zhivotnykh]. Moscow: KolosS.
ISBN 5953200927. [in Russian]
