JVMBBS - 2020 - Vol. 6 - iss. 3

Journal for Veterinary Medicine, Biotechnology and Biosafety

Volume 6, Issue 3, September 2020, Pages 13–16

ISSN 2411-3174 (print version) ISSN 2411-0388 (online version)

METHOD OF BIOCHEMICAL CHANGE CORRECTIONS IN THE BOAR ORGANISMS WITH TOXICANT-INDUCED REPRODUCTIVE DYSFUNCTIONS

Naumenko S. V., Koshevoi V. I., Siehodin O. B.

Kharkiv State Zooveterinary Academy, Kharkiv, Ukraine, e-mail: frolka001@gmail.com

Citation for print version: Naumenko, S. V., Koshevoi, V. I. and Siehodin, O. B. (2020) ‘Method of biochemical change corrections in the boar organisms with Toxicant-induced reproductive dysfunctions’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 6(3), pp. 13–16.

Download PDF (online version)

Citation for online version: Naumenko, S. V., Koshevoi, V. I. and Siehodin, O. B. (2020) ‘Method of biochemical change corrections in the boar organisms with Toxicant-induced reproductive dysfunctions’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 6(3), pp. 13–16. DOI: 10.36016/JVMBBS-2020-6-3-3.

Summary. The pathogenetic mechanism of reproductive diseases is oxidative stress, which is manifested by an increase in lipid peroxidation and a decrease in antioxidant potential. The aim of the study was to develop a method for the correction of biochemical changes in the body of boars with toxicant-induced reproductive dysfunctions using drugs based on nanobiomaterials, based on reducing lipoperoxidation, neutralization of toxic substances by antioxidant protection increasing of animals and endocrine activity stimulating of their gonads. The article presents the results of research on the effectiveness of the complex drug ‘Karafand+OV,Zn’, which contains carotenoids, phytoandrogens and nanomaterials — nanoparticles of gadolinium orthovanadate, activated by europium, and zinc carbonate. Experimental toxicant-induced reproductive dysfunctions were caused by feeding sodium nitrate at a dose of 0.3 g NO₃^–/kg body weight. The drug was administered in a dose of 15 ml per male, orally, once a day for 14 days. Blood samples for test were taken before and on the 20^th day after drug administration. Conventional biochemical methods were used, as well as chemiluminometry and enzyme-linked immunosorbent assay. There was a positive effect of the developed drug on the hormonal state (testosterone concentration increased by 91.8% (20.6 ± 0.32 nmol/l, p < 0.001), the content of vitamin A increased 1.3 times (0.65 ± 0.02 μmol/l, p < 0.001) and zinc by 47.6% (24.8 ± 0.86 μmol/l, p < 0.001), the dynamics of lipoperoxidation processes (the concentration of malonic dialdehyde in the serum was reduced by 53.2% (0.394 ± 0.01 μmol/l, p < 0.001)) and the system of antioxidant protection of boars (increased activity of catalase and superoxide dismutase in serum by 71.5% (41.4 ± 1.03 μmol/H₂O₂/l-min, p < 0.001) and 54.8% (8.98 ± 0.09 st. un./mgHb, p < 0.001), respectively), increased the content of reduced glutathione by 23.2%, indicators of the oxygen metabolism system (concentration 2,3-diphosphoglycerate increased 1.3 times (1.4 ± 0.03 mmol/l, p < 0.001), the activation of which reduces the hypoxic state. The total antioxidant activity of boars increased, as evidenced by a decrease in the light sum of chemiluminescence of serum by 47.6% (4.4 ± 0.15 un., p < 0.001). The results of research convincingly testify to the high efficiency of the use of the complex drug ‘Karafand+OV,Zn’ as a means of correction of toxicant-induced reproductive dysfunctions in boars and proves the possibility of its use in practical veterinary andrology

Keywords: complex drug, Karafand+OV,Zn, nanobiomaterials, lipoperoxidation, antioxidant protection, oxygen metabolism

References:

Agarwal, A., Mulgund, A., Sharma, R. and Sabanegh, E. (2014) ‘Mechanisms of oligozoospermia: an oxidative stress perspective’, Systems Biology in Reproductive Medicine, 60(4), pp. 206–216. doi: https://doi.org/10.3109/19396368.2014.918675

Chornozub, T. V. (2013) Influence of Antioxidant System Condition on Sperm Quality of Breeding Boars and Its Correction [Vplyv stanu antyoksydantnoi systemy na yakist spermy knuriv-plidnykiv ta yoho korektsiia]. The dissertation thesis for the scientific degree of the candidate of veterinary sciences. Sumy: Sumy National Agrarian University. [in Ukrainian]

Danchuk, O. V., Karpovskyi, V. I. and Danchuk, V. V. (2016) ‘Indices of lipid peroxidation intensity in pigs under the influence of stress factors’ [Indeksy intensyvnosti peroksydnoho okysnennia lipidiv u svynei za dii stresovoho faktora], Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies named after S. Z. Gzhytskyj. Series: Veterinary Sciences [Naukovyi visnyk Lvivskoho natsionalnoho universytetu veterynarnoi medytsyny ta biotekhnolohii imeni S. Z. Gzhytskoho. Seriia: Veterynarni nauky], 18(1.2), pp. 47–50. Available at: http://nbuv.gov.ua/UJRN/nvlnu_2016_18_1(2)__10. [in Ukrainian]

De Celis, R., Pedrón-Nuevo, N. and Feria-Velasco, A. (1996) ‘Toxicology of male reproduction in animals and humans’, Archives of Andrology, 37(3), pp. 201–218. doi: https://doi.org/10.3109/01485019608988523

Dubinina, E. E., Babenko, G. A., Shcherbak, I. G. and Turkin, V. B. (1990) ‘Characteristics of superoxide dismutase of human blood plasma’, Free Radical Biology and Medicine, 9(Suppl), p. 130. doi: https://doi.org/10.1016/0891-5849(90)90637-X

Fedorova, T. N., Korshunova, T. S. and Larsky, E. G. (1983) ‘Reactions with thiobarbituric acid for fluorometric determination of the blood malonic dialdehyde’ [Reaktsii s tiobarbiturovoy kislotoy dlya opredeleniya malonovogo dial’degida krovi metodom flyuorimetrii], Laboratory Science [Laboratornoe delo], 3, pp. 25–27. [in Russian]

Hunchak, V. M., Hufriy, D. F., Hutyy, B. V., Vasiv, R. O., Khariv, I. I., Khomik, R. I., Murska, S. D. and Guberuk, V. A. (2010) ‘Influence of sodium nitrate in toxic doses on system of antioxidant defense and lipid peroxidation in blood of bull-calves’ [Vplyv nitratu natriiu u toksychnykh dozakh na systemu antyoksydantnoho zakhystu ta perekysne okysnennia lipidiv u krovi buhaitsiv], The Animal Biology [Biolohiia tvaryn], 12(1), pp. 151–158. Available at: http://nbuv.gov.ua/UJRN/bitv_2010_12_1_25. [in Ukrainian]

Jankowiak, D., Pilarczyk, R., Drozd, R., Pilarczyk, B., Tomza-Marciniak, A., Wysocka, G., Rząd, I., Drozd, A. and Kuba, J. (2015) ‘Activity of antioxidant enzymes in the liver of wild boars (Sus scrofa) from aselenium-deficient area depending on sex, age, and season of the year’, Turkish Journal of Biology, 39(1), pp. 129–138. doi: https://doi.org/10.3906/biy-1405-52

Khariv, M. I., Gutyj, B. V., Butsyak, V. I. and Khariv, І. I. (2016) ‘Hematological indices of rat organisms under conditions of oxidative stress and liposomal preparation action’ [Hematolohichni pokaznyky orhanizmu shchuriv za umov oksydatsiinoho stresu ta za dii liposomalnoho preparatu], Biological Bulletin of Bogdan Chmelnitskiy Melitopol State Pedagogical University [Biolohichnyi visnyk Melitopolskoho derzhavnoho pedahohichnoho universytetu imeni Bohdana Khmelnytskoho], 6(1), pp. 276–289. doi: https://doi.org/10.15421/201615. [in Ukrainian]

Korolyuk, M. A., Ivanova, L. I., Mayorova, I. G. and Tokarev, V. E. (1988) ‘Determination of catalase activity’ [Opredelenie aktivnosti katalaz], Laboratory Science [Laboratornoe delo], 1, pp. 16–18. PMID: https://pubmed.ncbi.nlm.nih.gov/2451064. [in Russian]

Koshevoi, V. P., Naumenko, S. V., Koshevoi, V. I., Maliukin, Yu. V., Klochkov, V. K. and Kavok, N. S. (2015) ‘Computer monitoring of the indicators of structural and functional conditions of the reproductive system organs in males at deficiency of carotene (vitamin A) and zinc’ [Kompiuternyi monitorynh pokaznykiv strukturno-funktsionalnoho stanu orhaniv reproduktyvnoi systemy u samtsiv pry defitsyti karotynu (vitaminu A) ta Tsynku], Problems of Zooengineering and Veterinary Medicine [Problemy zooinzhenerii ta veterynarnoi medytsyny], 31(2), pp. 62–70. Available at: http://nbuv.gov.ua/UJRN/pzvm_2015_31(2)__16. [in Ukrainian]

Koshevoi, V. P., Fedorenko, S. Ya., Naumenko, S. V., Ivanchenko, M. M., Onyshchenko, O. V., Besedovska, K. S., Pasternak, A. M., Hladtsinova, I. O. Koshevoi, V. I., Skliarov, P. M., Maliukin, Yu. V., Yefimova, S. L. and Klochkov, V. K. (2016) Complex Preparations Based on Nano-Biomaterials and Their Use in Veterinary Reproductology: Methodological Recommendations [Kompleksni preparaty, stvoreni na osnovi nano-biomaterialiv, ta yikh vykorystannia u veterynarnii reproduktolohii: metodychni rekomendatsii]. Dnipropetrovsk: Porohy. [in Ukrainian]

Naumenko, S. V. (2020) ‘The state of oxygen metabolism system in males with gonadaldystrophy of the toxic type’, Actual Problems of Veterinary Biotechnology and Infectious Pathology of Animals: materials of the annual scientific and practic conference of young scientists, Kyiv, 9 July 2020 [Aktualni problemy veterynarnoi biotekhnolohii ta infektsiinoi patolohii tvaryn: materialy shchorichnoi naukovo-praktychnoi konferentsii molodykh vchenykh, Kyiv, 9 lypnia 2020 r.]. Kyiv: Komprynt, p. 24. Available at: http://ivm.kiev.ua/wp-content/uploads/Збірка-тез-конференції-2020.pdf

Rebrova, O. Yu. (2006) Statistical Analysis of Medical Data: Using of STATISTICA Applied Package [Statisticheskiy analiz meditsinskikh dannykh: primenenie paketa prikladnykh programm STATISTICA]. 3^rd ed. Moscow: MediaSfera. ISBN 5890840134. [in Russian]

Shostya, M. A., Pavlova, I. V., Chukhlib, Y. V., Kuzmenko, L. M., Kodak, T. S., Bereznytskyi, V. I. and Shaferivskyi, B. S. (2020) ‘The influence of humates on pro-oxidant-antioxidant homeostasis in breeding boars during heat stress’ [Vplyv humativ na prooksydantno-antyoksydantnyi homeostaz u knuriv-plidnykiv pid chas teplovoho stresu], Bulletin of Poltava State Agrarian Academy [Visnyk Poltavskoi derzhavnoi ahrarnoi akademii], 1, pp. 114–120. doi: https://doi.org/10.31210/visnyk2020.01.13. [in Ukrainian]

Tang, W., Wu, J., Jin, S., He, L., Lin, Q., Luo, F., He, X., Feng, Y., He, B., Bing, P., Li, T. and Yin, Y. (2020) ‘Glutamate and aspartate alleviate testicular/epididymal oxidative stress by supporting antioxidant enzymes and immune defense systems in boars’, Science China Life Sciences, 63(1), pp. 116–124. doi: https://doi.org/10.1007/s11427-018-9492-8

Vlizlo, V. V. (ed.) (2012) Laboratory Methods of Research in Biology, Animal Husbandry and Veterinary Medicine [Laboratorni metody doslidzhen u biolohii, tvarynnytstvi ta veterynarnii medytsyni]. Lviv: Spolom. ISBN 9769666656776. [in Ukrainian]