Journal for Veterinary Medicine, Biotechnology and Biosafety

Volume 7, Issue 4, December 2021, Pages 3–7

ISSN 2411-3174 (print version) ISSN 2411-0388 (online version)


Naumenko S. V. 1, Koshevoi V. I. 1, Skliarov P. M. 2, Klochkov V. K. 3, Yefimova S. L. 3

1 State Biotechnological University, Kharkiv, Ukraine, e-mail:

2 Dnipro State Agrarian and Economic University, Dnipro, Ukraine

3 Institute of Scintillation Materials of the National Academy of Sciences of Ukraine, Kharkiv, Ukraine

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Citation for print version: Naumenko, S. V., Koshevoi, V. I., Skliarov, P. M., Klochkov, V. K. and Yefimova, S. L. (2021) ‘Effectiveness of using the complex drug ‘Karafand+OV,Zn’ to increase the reproductive capacity of males of domestic animals’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 7(4), pp. 3–7.

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Citation for online version: Naumenko, S. V., Koshevoi, V. I., Skliarov, P. M., Klochkov, V. K. and Yefimova, S. L. (2021) ‘Effectiveness of using the complex drug ‘Karafand+OV,Zn’ to increase the reproductive capacity of males of domestic animals’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 7(4), pp. 3–7. DOI: 10.36016/JVMBBS-2021-7-4-1.

Summary. Experimental research on male domestic animals (boars, bulls) proved the effectiveness of using the complex drug ‘Karafand+OV,Zn’ to increase their reproductive capacity. This preparation contains carotenoids, biologically active substances from the rhizome of marsh calamus and nanoparticles of orthovanadate gadolinium activated by europium and zinc carbonate. It was observed that the introduction of the drug increased the activity of antioxidant protection — catalase activity in the serum and erythrocytes in boars by 15.9% (p < 0.01) and 11.4% (p < 0.05), in bulls — by 7.3% (p < 0.05) and 12.4% (p < 0.01), respectively, and SOD in boars by 30.2% (p < 0.01), in bulls — by 16.5% (P <0.05) — when reducing the intensity of lipoperoxidation processes (reducing the amount of TBA-AP in the serum and erythrocytes of males — in boars by 19.4% (p < 0.05) and 16.9% (p < 0.001), in bulls — by 25% (p < 0.05) and by 12.4% (p < 0.01), respectively. There was an improvement in sperm quality (especially in terms of motility and content of sperm with morphological abnormalities) and endocrine function of the testes — testosterone levels increased by 16.8% in boars (p < 0.05), and in bulls — by 44.3 % (p < 0.001). At the same time, there was an increase in protein-vitamin-mineral metabolism in the body: the amount of vitamin A reliably increased in boars by 14.5% (p < 0.001), in bulls — by 7.6% (p < 0.05), and the content of zinc — in boars by 36.5% (p < 0.001), in bulls — by 16.0% (p < 0.01), the amount of total protein, calcium and phosphorus also increased. Thus, the obtained results allow us to recommend the complex drug ‘Karafand+OV,Zn’ as an effective means for increasing the reproductive capacity of males with pronounced sperm-modeling and androgen-stimulating effects and powerful antioxidant properties

Keywords: reproductive system, lipoperoxidation, nanoparticles, biologically active substances, sperm quality, hormonal background


Agarwal, A., Makker, K. and Sharma, R. (2007) ‘Clinical relevance of oxidative stress in male factor infertility: An update: Oxidative stress and male infertility’, American Journal of Reproductive Immunology, 59(1), pp. 2–11. doi: 10.1111/j.1600-0897.2007.00559.x.

Agarwal, A., Rana, M., Qiu, E., Al Bunni, H., Bui, A. D. and Henkel, R. (2018) ‘Role of oxidative stress, infection and inflammation in male infertility’, Andrologia, 50(11), p. e13126. doi: 10.1111/and.13126.

Barik, G., Chaturvedula, L. and Bobby, Z. (2019) ‘Role of oxidative stress and antioxidants in male infertility: An interventional study’, Journal of Human Reproductive Sciences, 12(3), p. 204. doi: 10.4103/jhrs.JHRS_135_18.

Chornozub, T. V. (2013) Influence of Antioxidant System Condition on Sperm Quality of Breeding Boars and Its Correction [Vplyv stanu antyoksydantnoi systemy na yakist spermy knuriv-plidnykiv ta yoho korektsiia]. The dissertation thesis for the scientific degree of the candidate of veterinary sciences. Sumy: Sumy National Agrarian University. [in Ukrainian].

Dziekońska, A., Świąder, K., Koziorowska-Gilun, M., Mietelska, K., Zasiadczyk, Ł. and Kordan, W. (2017) ‘Effect of boar ejaculate fraction, extender type and time of storage on quality of spermatozoa’, Polish Journal of Veterinary Sciences, 20(1), pp. 77–84. doi: 10.1515/pjvs-2017-0011.

Hunchak, V. M., Hufriy, D. F., Hutyy, B. V., Vasiv, R. O., Khariv, I. I., Khomik, R. I., Murska, S. D. and Guberuk, V. A. (2010) ‘Influence of sodium nitrate in toxic doses on system of antioxidant defense and lipid peroxidation in blood of bull-calves’ [Vplyv nitratu natriiu u toksychnykh dozakh na systemu antyoksydantnoho zakhystu ta perekysne okysnennia lipidiv u krovi buhaitsiv], The Animal Biology [Biolohiia tvaryn], 12(1), pp. 151–158. Available at: [in Ukrainian].

Karpenko, N. O., Korenieva, Ye. M., Chystiakova, E. Ye., Smolienko, N. P., Bielkina, I. O., Kustova, S. P., Boiko, M. O., Kavok, N. S., Medvedovska, N. V. and Кlochkov, V. K. (2020) ‘The studying of the prostateprotective and spermmodulating effects of the nanoparticles based on rare-earth elements oxides’ [Doslidzhennia prostatoprotektornoi ta spermomodeliuiuchoi dii nanochastynok na osnovi oksydiv ridkisnozemelnykh elementiv], Problems of Endocrine Pathology [Problemy endokrynnoi patolohii], 73(3), pp. 109–118. doi: 10.21856/j-PEP.2020.3.14. [in Ukrainian].

Koshevoi, V. P., Naumenko, S. V., Koshevoi, V. I., Maliukin, Yu. V., Klochkov, V. K. and Kavok, N. S. (2015) ‘Computer monitoring of the indicators of structural and functional conditions of the reproductive system organs in males at deficiency of carotene (vitamin A) and zinc’ [Kompiuternyi monitorynh pokaznykiv strukturno-funktsionalnoho stanu orhaniv reproduktyvnoi systemy u samtsiv pry defitsyti karotynu (vitaminu A) ta Tsynku], Problems of Zooengineering and Veterinary Medicine [Problemy zooinzhenerii ta veterynarnoi medytsyny], 31(2), pp. 62–70. Available at: [in Ukrainian].

Koshevoi, V. P., Fedorenko, S. Ya., Naumenko, S. V., Ivanchenko, M. M., Onyshchenko, O. V., Besedovska, K. S., Pasternak, A. M., Hladtsinova, I. O. Koshevoi, V. I., Skliarov, P. M., Maliukin, Yu. V., Yefimova, S. L. and Klochkov, V. K. (2016) Complex Preparations Based on Nano-Biomaterials and Their Use in Veterinary Reproductology: Methodological Recommendations [Kompleksni preparaty, stvoreni na osnovi nano-biomaterialiv, ta yikh vykorystannia u veterynarnii reproduktolohii: metodychni rekomendatsii]. Dnipropetrovsk: Porohy. [in Ukrainian].

Koshevoy, V. I. and Naumenko, S. V. (2020) ‘The activity of the antioxidant protection enzymatic system of boars with a decrease in their reproductive capacity under oxidative stress’, Theoretical and Applied Veterinary Medicine, 8(3), pp. 194–197. doi: 10.32819/2020.83027.

Koshevoy, V. I., Naumenko, S. V., Klochkov, V. K. and Yefimova, S. L. (2021) ‘The use of gadolinium orthovanadate nanoparticles for the correction of reproductive ability in boars under oxidative stress’, Ukrainian Journal of Veterinary Sciences, 12(2), pp. 74–82. doi: 10.31548/ujvs.2021.02.008.

Otasevic, V., Stancic, A., Korac, A., Jankovic, A. and Korac, B. (2020) ‘Reactive oxygen, nitrogen, and sulfur species in human male fertility. A crossroad of cellular signaling and pathology’, BioFactors, 46(2), pp. 206–219. doi: 10.1002/biof.1535.

Palani, A. F. (2018) ‘Effect of serum antioxidant levels on sperm function in infertile male’, Middle East Fertility Society Journal, 23(1), pp. 19–22. doi: 10.1016/j.mefs.2017.07.006.

Piomboni, P., Gambera, L., Serafini, F., Campanella, G., Morgante, G. and De Leo, V. (2008) ‘Sperm quality improvement after natural anti-oxidant treatment of asthenoteratospermic men with leukocytospermia’, Asian Journal of Andrology, 10(2), pp. 201–206. doi: 10.1111/j.1745-7262.2008.00356.x.

Rebrova, O. Yu. (2006) Statistical Analysis of Medical Data: Using of STATISTICA Applied Package [Statisticheskiy analiz meditsinskikh dannykh: primenenie paketa prikladnykh programm STATISTICA]. 3rd ed. Moscow: MediaSfera. ISBN 5890840134. [in Russian].

Ribas-Maynou, J. and Yeste, M. (2020) ‘Oxidative stress in male infertility: causes, effects in assisted reproductive techniques, and protective support of antioxidants’, Biology, 9(4), p. 77. doi: 10.3390/biology9040077.

Ritchie, C. and Ko, E. Y. (2021) ‘Oxidative stress in the pathophysiology of male infertility’, Andrologia, 53(1), p. e13581. doi: 10.1111/and.13581.

Sabeti, P., Pourmasumi, S., Rahiminia, T., Akyash, F. and Talebi, A. R. (2016) ‘Etiologies of sperm oxidative stress’, International Journal of Reproductive BioMedicine, 14(4), pp. 231–240. doi: 10.29252/ijrm.14.4.231.

Tvrda, E., Mackovich, A., Greifova, H., Hashim, F. and Lukac, N. (2017) ‘Antioxidant effects of lycopene on bovine sperm survival and oxidative profile following cryopreservation’, Veterinární Medicína, 62(8), pp. 429–436. doi: 10.17221/86/2017-VETMED.

Vlizlo, V. V. (ed.) (2012) Laboratory Methods of Research in Biology, Animal Husbandry and Veterinary Medicine [Laboratorni metody doslidzhen u biolohii, tvarynnytstvi ta veterynarnii medytsyni]. Lviv: Spolom. ISBN 9769666656776. [in Ukrainian].

Yaremchuk, I., Kuzmina, N., Ostapiv, D., Sharan, М. and Kava, S. (2017) ‘Oxidative processes intensity and quality of bull semen when adding microelements nanosuccinate compounds’ [Intensyvnist okysnykh protsesiv ta yakist spermiiv buhaiv za dodavannia v rozridzhuvach nanosuktsynativ mikroelementiv], Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies named after S. Z. Gzhytskyj. Series: Veterinary Sciences [Naukovyi visnyk Lvivskoho natsionalnoho universytetu veterynarnoi medytsyny ta biotekhnolohii imeni S. Z. Gzhytskoho. Seriia: Veterynarni nauky], 19(77), pp. 185–189. doi: 10.15421/nvlvet7740. [in Ukrainian].