Issue 1

Journal for Veterinary Medicine, Biotechnology and Biosafety

Volume 1, Issue 1, March 2015, Pages 9–14

ISSN XXXX-XXXX (print version) ISSN ISSN 2411-0388 (online version)

PHYLOGENETIC AND MOLECULAR GENETIC STUDIES OF THE ANIMALS ARTERIVIRUSES

Gerilovych A. P., Limanska O. Yu., Gema I. O., Rudova N. G., Smolyaninova Ye. A.

National Scientific Center “Institute of Experimental and Clinical Veterinary Medicine”, Kharkiv, Ukraine, e-mail: antger@vet.kharkov.ua

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Citation for print version: Gerilovych, A. P., Limanska, O. Yu., Gema, I. O., Rudova, N. G. and Smolyaninova, Ye. A. (2015) ‘Phylogenetic and molecular genetic studies of the animals arteriviruses’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 1(1), pp. 9–14.

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Citation for online version: Gerilovych, A. P., Limanska, O. Yu., Gema, I. O., Rudova, N. G. and Smolyaninova, Ye. A. (2015) ‘Phylogenetic and molecular genetic studies of the animals arteriviruses’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 1(1), pp. 9–14. Available at: http://jvmbbs.kharkov.ua/archive/2015/volume1/issue1/jvmbbso2015011_009-014.pdf

Summary. This study was aimed to provide the phylogenetic characteristics of arteriviruses of the different species, and to perform PCR-based surveillance of the porcine diseases, caused by PRRS virus and its associates.

The study was conducted based on the molecular genetics methods, including PCR, phylogenetic analysis of sequences of the different arteriviral species.

The phylogenetic relationships of the porcine reproductive and respiratory syndrome virus circulating in different geographical regions were studied. The availability of the phylogenetic analysis for genotyping was performed and microorganism molecular markers were demonstrated. The monitoring of the pathogen PRRS spread in the farms of Eastern Ukraine was conducted.

Variability of genes encoding glycoproteins GP2, GP3, GP4 and GP5 of the arteriviruses of animals was demonstrated and allowed to recommend these for PRRSV molecular epidemiology study. The monitoring spread of the PRRS virus in the farms in the Eastern Ukraine demonstrated the possibility of PRRS virus association with porcine circovirus type 2.

Keywords: porcine reproductive and respiratory syndrome virus, real-time polymerase chain reaction, phylogenetic analysis.

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