Issue 1
Journal
for Veterinary Medicine, Biotechnology and Biosafety
Volume 1, Issue
1, March 2015, Pages 15–22
ISSN XXXX-XXXX (print version) ISSN ISSN 2411-0388 (online version)
CREATION MOLECULAR-GENETIC CONTROL SYSTEM OF PESTIVIRUS CONTAMINATION IN BIOTECHNOLOGY OBJECTS
Stegniy B.
T.,
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PDF (print version)
Citation for print version: Stegniy, B. T., Goraichuk, I. V., Gerilovych, A.
P., Kucheryavenko, R. O., Bolotin,
V. I. and Solodiankin, O. S. (2015) ‘Creation molecular-genetic
control system of Pestivirus contamination in
biotechnology objects’, Journal for
Veterinary Medicine, Biotechnology and Biosafety, 1(1), pp. 15–22.
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PDF (online version)
Citation for online version: Stegniy, B. T., Goraichuk, I. V., Gerilovych, A.
P., Kucheryavenko, R. O., Bolotin,
V. I. and Solodiankin, O. S. (2015) ‘Creation molecular-genetic
control system of Pestivirus contamination in
biotechnology objects’, Journal for
Veterinary Medicine, Biotechnology and Biosafety. [Online] 1(1),
pp. 15–22. Available at: http://jvmbbs.kharkov.ua/archive/2015/volume1/issue1/jvmbbso2015011_015-022.pdf
Summary. This
study aimed on (i) creation molecular-genetic control
system of pestivirus contamination in biotechnology
objects, (ii) identification of persistently infected with bovine viral diarrhea virus (BVDV) animals and (iii) genetic typing of selected BVDV isolates.
RNA extraction,
cloning, polymerase chain reaction (PCR), real-time PCR, enzyme-linked immunosorbent
assay, serum neutralization test, sequencing.
It was shown that we had constructed
the recombinant plasmids with insertion Erns
gene fragment (826 base pair) of BVDV-1 and
BVDV-2. Also we had developed and optimized
parameters of duplex PCR for the simultaneous
indication Mollicutes DNA and BVDV
RNA, with the possibility of nested PCR for further
identification of BVDV genotypes. Specific BVDV antibodies were detected in 725 of 1042 (69.6 %) analyzed
samples. In this study 5 persistently infected with BVDV
animals were detected in farms B and C of Kharkiv
region. The genetic typing of viral isolates
revealed that only BVDV-1 viruses were present. The phylogenetic analysis confirmed two BVDV-1
subtypes, namely b and f and revealed that all viruses from the farm B of Kharkiv region and from biotechnological objects were typed
as BVDV-1b, but virus from the farm C of Kharkiv region and farm of
The obtained recombinant plasmids
can be used as a positive control for PCR and
test-system for control of pestivirus contamination
in biotechnology objects. Our results indicated that the BVDV
infection is widespread in cattle herds in the eastern
Keywords: bovine viral diarrhea virus, pestivirus
contamination, cloning, pTZ57R/T, restriction
enzyme digestion analysis, ELISA, SNT, PCR, real-time PCR, genotyping, phylogenetic analysis.
References:
Bolin, S. R.,
Lim, A., Grotelueschen, D. M., McBeth,
W. W. and Cortese, V. S. (2009) ‘Genetic
characterization of bovine viral diarrhea viruses isolated from persistently
infected calves born to dams vaccinated against bovine viral diarrhea virus before
breeding’, American Journal of Veterinary Research, 70(1), pp.
86–91. doi: http://dx.doi.org/10.2460/ajvr.70.1.86
Boom, R., Sol,
C. J., Salimans, M. M., Jansen, C. L., Wertheim-van Dillen, P. M. and van der Noordaa, J. (1990) ‘Rapid and simple method for
purification of nucleic acids’, Journal of Clinical Microbiology,
28(3), pp. 495–503. Available at: http://jcm.asm.org/content/28/3/495.full.pdf
Canal, C. W., Strasser, M., Hertig, C., Masuda, A. and Peterhans,
E. (1998) ‘Detection of antibodies to bovine viral diarrhoea
virus (BVDV) and characterization of genomes of BVDV from
Chase, C. C. L.,
Elmowalid, G. and Yousif,
A. A. A. (2004) ‘The immune response to bovine
viral diarrhea virus: a constantlychanging
picture’, Veterinary Clinics of North America: Food Animal Practice,
20(1), pp. 95–114. doi:
http://dx.doi.org/10.1016/j.cvfa.2003.11.004
Heinz, F. X., Collett, M. S., Purcell, R. H., Gould, E. A., Howard, C.
R., Houghton, M., Moormann, R. J. M., Rice, C. M. and
Thiel, H.-J. (2000) ‘Family Flaviviridae’
in van Regenmortel, C. M. F. M. H. V., Bishop, D. H.
L., Carstens, E. B., Estes, M. K., Lemon, S. M., Maniloff, J., Mayo, M. A., McGeoch,
D. J., Pringle, C. R. and Wickner, R. B. (eds) Virus taxonomy: Seventh report of the International
Committee on Taxonomy of Viruses.
Hessman, B. E., Fulton, R.
W., Sjeklocha, D. B., Murphy, T. A., Ridpath, J. F. and Payton, M. E. (2009) ‘Evaluation
of economic effects and the health and performance of the general cattle
population after exposure to cattle persistently infected with bovine viral diarrhea
virus in a starter feedlot’, American Journal of Veterinary Research,
70(1), pp. 73–85. doi:
http://dx.doi.org/10.2460/ajvr.70.1.73
Houe, H. (1999) ‘Epidemiological features and economical importance of
bovine virus diarrhoea virus (BVDV)
infections’, Veterinary Microbiology, 64(2-3), pp. 89–107. doi: http://dx.doi.org/10.1016/s0378-1135(98)00262-4
Jackova, A., Novackova, M., Pelletier, C., Audeval,
C., Gueneau, E., Haffar,
A., Petit, E., Rehby, L. and Vilcek,
S. (2007) ‘The extended genetic diversity of BVDV-1: Typing of BVDV isolates
from
Kennedy, J. A. (2006) ‘Diagnostic efficacy of a reverse
transcriptase–polymerase chain reaction assay to screen cattle for
persistent bovine viral diarrhea virus infection’, Journal of the
American Veterinary Medical Association, 229(9), pp. 1472–1474. doi: http://dx.doi.org/10.2460/javma.229.9.1472
Kong, F., James,
G., Gordon, S., Zelynski, A. and Gilbert, G. L.
(2001) ‘Species-specific PCR for identification
of common contaminant mollicutes in cell
culture’, Applied and Environmental Microbiology, 67(7), pp.
3195–3200. doi: http://dx.doi.org/10.1128/aem.67.7.3195-3200.2001
Larson, L. R., Grotelueschen, D. M., Brock, K. V., Hunsaker,
B. D., Smith, R. A., Sprowls, R. W., MacGregor, D. S., Loneragan, G.
H. and Dargatz, D. A. (2004) ‘Bovine Viral
Diarrhea (BVD): Review for Beef Cattle
Veterinarians’, Bovine Practitioner, 38(1), pp. 93–102.
Available at: http://www.aabp.org/members/publications/2004/prac_feb_04/feb04_14.pdf
Nettleton, P. F. and Entrican, G. (1995)
‘Ruminant pestiviruses’, British
Veterinary Journal, 151(6), pp. 615–642. doi: http://dx.doi.org/10.1016/s0007-1935(95)80145-6
Peterhans, E., Bachofen, C., Stalder, H. and Schweizer, M. (2010) ‘Cytopathic
bovine viral diarrhea viruses (BVDV): emerging pestiviruses doomed to extinction’, Veterinary
Research, 41(6), pp. 44–57. doi:
http://dx.doi.org/10.1051/vetres/2010016
Sanger, F. (2001) ‘The early days of DNA sequences’, Nature
Medicine, 7(3), pp. 267–268. doi: http://dx.doi.org/10.1038/85389
Ståhl, K. and Alenius, S. (2012) ‘BVDV
control and eradication in
Steck, F., Lazary, S., Fey, H., Wandeler,
A., Huggler, C., Oppliger,
G., Baumberger, H., Kaderli,
R. and Martig, J. (1980) ‘Immune responsiveness
in cattle fatally affected by bovine virus diarrhea-mucosal disease’, Zentralblatt für Veterinärmedizin Reihe B,
27(6), pp. 429–445. doi: http://dx.doi.org/10.1111/j.1439-0450.1980.tb01790.x
Studer, E., Bertoni, G. and Candrian,
U. (2002) ‘Detection and characterization of Pestivirus
contaminations in human live viral vaccines’, Biologicals,
30(4), pp. 289–296. doi: http://dx.doi.org/10.1006/biol.2002.0343
Sullivan, D. G. and Akkina, R. K. (1995)
‘A nested polymerase chain reaction assay to differentiate pestiviruses’, Virus Research, 38(2-3), pp.
231–239. doi: http://dx.doi.org/10.1016/0168-1702(95)00065-x
Sung, H., Kang,
S. H., Bae, Y. J., Hong, J. T., Chung, Y. B., Lee, C.
K. and Song, S. (2006) ‘PCR-based detection of Mycoplasma species’, Journal of Microbiology,
44(1), pp. 42–49. Available at: http://www.msk.or.kr/inc/download_FIDX.asp?FTYPE=5&FIDX=2338
Uphoff, C. C. and Drexler, H. G. (2002) ‘Detection of mycoplasma in leukemia–lymphoma cell lines using
polymerase chain reaction’, Leukemia, 16(2), pp. 289–293. doi: http://dx.doi.org/10.1038/sj.leu.2402365
Van Kuppeveld, F. J., Van der Logt, J. T., Angulo, A. F., van Zoest, M. J., Quint, W. G., Niesters, H. G., Galama, J. M.
and Melchers, W. J. (1992) ‘Genus- and species-specific identification of
mycoplasmas by 16S rRNA
amplification’, Applied and Environmental Microbiology, 58(8), pp.
2606–2615. Available at: http://aem.asm.org/content/58/8/2606.full.pdf
Vilček, S., Herring, A. J.,
Herring, J. A., Nettleton, P. F., Lowings, J. P. and
Paton, D. J. (1994) ‘Pestiviruses isolated from
pigs, cattle and sheep can be allocated into at least three genogroups
using polymerase chain reaction and restriction endonuclease
analysis’, Archives of Virology, 136(3-4), pp. 309–323. doi: http://dx.doi.org/10.1007/BF01321060
Vilcek, S., Durkovic, B., Kolesárová,
M., Greiser-Wilke,
