Issue 2
Journal
for Veterinary Medicine, Biotechnology and Biosafety
Volume 1, Issue 2,
June 2015, Pages 15–21
ISSN 2411-3174 (print version) ISSN 2411-0388 (online version)
INfLuENCE Of PHYTONuTRIENT ‘VITASTIm’ ON
CHICKEN muCOSAL ImmuNITY AfTER INfECTION wITH LOw-PATHOLOGICAL AVIAN INfLuENZA
VIRuS
Stegniy B. T.,
Shutchenko P. O., Muzyka D. V., Kovalenko L. V., Medvid K. O., Stegniy M. Yu.,
Obukhovska O. V.
Lillehoj H. S.
Animal Parasitic
Diseases Laboratory, Animal and Natural Resources Institute, Beltsville
Agricultural Research Center, Beltsville, Maryland, USA
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PDF (print version)
Citation for print version: Stegniy B. T., Shutchenko P. O., Muzyka D. V.,
Kovalenko L. V., Medvid K. O., Stegniy M. Yu., Obukhovska O. V. and Lillehoj H.
S. (2015) ‘Influence of phytonutrient ‘Vitastim’ on chicken
mucosal immunity after infection with low-pathological avian influenza virus’,
Journal for Veterinary Medicine, Biotechnology
and Biosafety, 1(2), pp. 15–21.
Download
PDF (online version)
Citation for online version: Stegniy B. T., Shutchenko P. O., Muzyka D.
V., Kovalenko L. V., Medvid K. O., Stegniy M. Yu., Obukhovska O. V. and Lillehoj
H. S. (2015) ‘Influence of phytonutrient ‘Vitastim’ on
chicken mucosal immunity after infection with low-pathological avian influenza
virus’, Journal for Veterinary
Medicine, Biotechnology and Biosafety. [Online] 1(2), pp. 15–21.
Available at:
http://jvmbbs.kharkov.ua/archive/2015/volume1/issue2/oJVMBBS_2015012_015-021.pdf
Summary.
The aim of our study was to investigate the immunostimulatory effect of
phytonutrient ‘Vitastim’ on the immune response after avian
infection with low pathogenic avian influenza virus.
It was
conducted three groups of experimental chickens of 6 week old (21 birds in each
group) to study the mucosal immunity of chickens after experimental infection
with low-pathological avian influenza virus, and application of phytonutrient
‘Vitastim’. Cryostat sections of 7-μm thickness were prepared,
and detection of cells was done using unlabeled primary monoclonal antibodies
against the CD4, CD8, IgM, IgG, IgA, macrophage antigens, and a commercially
available staining kit. As a negative control, slides were incubated with PBS
instead of the monoclonal antibodies. Sections were counterstained with
hematoxylin and mounted with Canada balsam. Statistical analysis was performed
using SPSS 17.0 software for Windows.
By the
results of immunohistochemical research of influence of immunostimulating
phytogenous preparation ‘Vitastim’ on organism of chickens there
was determined that given preparation more actively influence on humoral cell
of immune reaction in norm and at lowly pathogenic avian influenza (caeca,
trachea, lungs) that testified more intensive formation and accumulation of
B-lymphocytes which produce immunoglobulins. At research of spleen, there was
determined amplified proliferation of T-lymphocytes, macrophages that
characterize the activation of cell immune reaction.
The
results of immunohistochemical studies of inner organs of chickens of 3
research groups, it was established the influence of the phytonutrient
‘Vitastim’ on humoral immune system, as evidenced by high levels of
IgM, IgG, IgA. ‘Vitastim’ had stimulating impact on state of cell
immunity, however, as evidenced by the low levels of CD4, macrophages, in
chickens of experimental groups compared to the intact birds, cell immunity
does not play a significant role in the pathogenesis of LPAI.
Keywords:
avian influenza virus, cell-, and humoral-mediated immune response,
immunohistochemistry, low pathogenic avian influenza
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