Issue 2
Journal for Veterinary Medicine, Biotechnology and Biosafety
Volume 1, Issue 2, June
2015, Pages 32–33
ISSN 2411-3174
(print version) ISSN 2411-0388 (online version)
DEVELOPMENT
OF SOFTWARE FOR CALCULATING THE BIOLOGICAL ACTIVITY OF MYCOBACTERIAL ALLERGENS
Bilushko V. V.,
Zavgorodniy A. I.
Bilushko E. V.
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PDF (print version)
Citation for print version: Bilushko V. V., Zavgorodniy
A. I. and Bilushko E. V. (2015) ‘Development of software for calculating
the biological activity of mycobacterial allergens’, Journal for Veterinary Medicine, Biotechnology and Biosafety,
1(2), pp. 32–33.
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PDF (online version)
Citation for
online version: Bilushko V. V., Zavgorodniy A. I. and Bilushko E. V. (2015)
‘Development of software for calculating the biological activity of
mycobacterial allergens’, Journal for Veterinary Medicine, Biotechnology and Biosafety.
[Online] 1(2), pp. 32–33. Available at:
http://jvmbbs.kharkov.ua/archive/2015/volume1/issue2/oJVMBBS_2015012_032-033.pdf
Summary. The paper contains the results of the development
and production testing of the software tool calculation for biological activity
of mycobacterial allergens for veterinary use.
Purpose — to
develop the software for calculating of the biological activity of
mycobacterial allergens, and conduct it's testing of industrial development in
the catch during the control of commercial series of tuberculin purified (PPD)
for mammals (n = 28).
Software design system was
used cba platform .NET
Framework 4.5, the programming language C# 5.0, the development
environment Microsoft Visual Studio 2013, as well as WPF technology. Clinical
studies were performed under conditions of Sumy State Biofactory by the
parallel calculation of tuberculin purified activity (PPD) for mammals in the
standard solution (series number = 28) using the developed program cba and
calculation method of biological activity according to the current TC U
24.4-00497087-107:2011 ‘Tuberculіn dry purified (PPD) for mammals
(National standard)’.
As a result, the
calculation of the biological activity of 28 experimental series of
tuberculin for mammals according to the procedure specified in regulatory
documents and with the help of the developed software cba demonstrated activity of the
preparation at the level 53 746 U/cm3 in standard
solution.
The computer program cba for the determination of the
biological activity of mycobacterial allergens provides accurate and timely
calculation of the amount of unit content in the volume 1.0 cm3
of the standard solution of purified tuberculin (PPD) for mammals.
Perspectives for future
research will include conduction of the further testing of the software for
allergen dry purified from atypical mycobacteria (AAM) and avian purified
tuberculin (PPD) in the production environment, as well as in the development
of targeted programs for different operating processes that require complex
mathematical calculations in the manufacture process of different preparation
dilutions, as the conditions of the producing and for research purpose.
Keywords: biological activity, software, units (U), mycobacterial
allergens, tuberculin
References:
Kozlov,
V., Bezgin, V. and Shumilov, K. (2004) ‘Evaluation of the activity of the
national standard tuberculin (PPD) for mammals with respect to the 1st
international standard tuberculin (PPD) bovine’ [Otsenka aktivnosti
natsional'nogo standarta tuberkulina (PPD) dlya mlekopitayushchikh otnositel'no
1-go mezhdunarodnogo standarta tuberkulina (PPD)]. Veterinary pathology [Veterinarnaya
patologiya], 1–2, pp. 85–89. [in
Russian]
Kozlov, V. (2007) Allergens for diagnosis of tuberculosis. Improvement
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for the doctor’s degree in veterinary science. [in
Russian]
Zavgorodniy, A.,
Tikhonov, P., Degtyarev, I. and Skrypnik, V. (2006) ‘PPD‑tuberculіn
for mammals made by
TC U
24.4‑00497087‑107 (2011) ‘Tuberculіn
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sukhyi ochyshchenyi dlia ssavtsiv. Natsionalnyi
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Troelsen,
A., (2013) Pro C# 5.0 and the
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