Issue 3
Journal for Veterinary Medicine, Biotechnology and Biosafety
Volume
1, Issue 3, September 2015, Pages 14–16
ISSN 2411-3174 (print version) ISSN 2411-0388
(online version)
BIOLOGICAL PROPERTIES OF CAMPYLYLOBACTER
MUSEUM STRAINS AFTER LONG STORAGE
IN LYOPHILIZED FORM
Obukhovska O. V., Kalinichenko
T. V., Dragut S. S., Kutzenko
V. A.
National
Scientific Center ‘Institute of Experimental and Clinical Veterinary
Medicine’, Kharkiv, Ukraine, e-mail: olgaobukhovska@gmail.com
Download
PDF (print version)
Citation for print version: Obukhovska, O. V., Kalinichenko, T. V., Dragut, S.
S. and Kutzenko, V. A. (2015) ‘Biological properties
of Campylylobacter
museum strains after long storage in lyophilized form’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 1(3), pp. 14–16.
Download
PDF (online version)
Citation for online version: Obukhovska,
O. V., Kalinichenko, T. V., Dragut,
S. S. and Kutzenko, V. A. (2015) ‘Biological properties of Campylylobacter museum strains
after long storage in lyophilized form’, Journal for
Veterinary Medicine, Biotechnology and Biosafety.
[Online] 1(3), pp. 14–16. Available at: http://jvmbbs.kharkov.ua/archive/2015/volume1/issue3/oJVMBBS_2015013_014-016.pdf
Summary. Campylobacteriosis is
dangerous disease of agricultural animals, which is characterized
by lesions of the reproductive system and the gastrointestinal tract.
Contaminated livestock products can be a source of infection for humans and
cause toxicoinfection with severe disease. Domestic preparations for the in vivo diagnosis of the disease is
missing. The main condition for the creation of effective diagnostics and
vaccines is the presence of stable production strains. An important focus in
the work of veterinary microbiologists is the studying biological properties of
Campylobacter museum strains and
definition of the capacity for preservation of these properties during long
storage. We conducted experiments for determine of museum Campylobacter cultures viability, that have been isolated from
animals and birds and were stored in lyophilized form for different terms. A total of 24 strains were studied. It has
been established that Campylobacter
lose about 1.0% of their life potential for every year of storage in
lyophilized form. After 10–12 years of storage only 44.4% strains retain
their viability, therefore it is not feasibly to keep them for a longer time
under such conditions. It has been shown the ability
to save typical properties of Campylobacter
strains within the specified retention period.
Keywords: Campylobacter strains, biological
properties, viability
References:
Babkin, A. F., Galishchev, N. I. and Novakovskiy, D. S. (2002) ‘Study
of survival rate of Campylobacter
stored in freeze-dried state and on nutrient
media’ [Izuchenie vyzhivaemosti kampilobakteriy, khranyashchikhsya v liofil’nom sostoyanii na pitatel>nykh
sredakh], Veterinary
Medicine [Veterynarna medytsyna], 80, pp.
42–47. [in Russian]
Babkin, A. F., Galishchev, N. I. and Novakovskiy, D. S. (2003) ‘Cultural-and-biochemical
properties of Campylobacteria and monospecific serum production’ [Kul’tural’no-biokhimicheskie
svoystva kampilobakteriy i poluchenie monospetsificheskikh
syvorotok], Veterinary
Medicine [Veterynarna medytsyna], 81, pp.
25–33. [in Russian]
Mshelia, G., Amin,
J., Woldehiwet, Z., Murray,
R. and Egwu, G. (2009)
‘Epidemiology of bovine venereal campylobacteriosis: geographic distribution and recent advances in molecular diagnostic
techniques’, Reproduction
in Domestic Animals, 45(5), pp. e221–e230. doi: http://dx.doi.org/10.1111/j.1439-0531.2009.01546.x
Van Bergen,
M. A. P., Linnane, S., van Putten, J. P. M. and Wagenaar, J. A. (2006) ‘Global
detection and identification of Campylobacter fetus subsp. venerealis’,
Revue scientifique
et technique (International Office of Epizootics), 24(3), pp. 1017–1026. Available at: http://web.oie.int/boutique/extrait/vanbergen10171026.pdf
Mai, H. M., Irons, P. C., Kabir, J. and Thompson, P. N. (2013)
‘Prevalence of bovine genital campylobacteriosis and trichomonosis of bulls in northern
Nigeria’, Acta
Veterinaria Scandinavica,
55(1). doi: http://dx.doi.org/10.1186/1751-0147-55-56
Molina, L., Perea,
J., Meglia, G., Angon, E. and Garcia, A. (2013) ‘Spatial and temporal
epidemiology of bovine trichomoniasis and bovine genital
campylobacteriosis in La Pampa province
(Argentina)’, Preventive
Veterinary Medicine,
110(3–4), pp. 388–394. doi: http://dx.doi.org/10.1016/j.prevetmed.2013.02.019
Swai, E. S.,
Hulsebosch, J. and Van der Heijden,
W. (2005) ‘Prevalence of
genital campylobacteriosis and trichomonosis in crossbred breeding
bulls kept on zero-grazed smallholder dairy farms in the
Tanga region of Tanzania’, Journal of the South African
Veterinary Association,
76(4), pp. 224–227. doi:
http://dx.doi.org/10.4102/jsava.v76i4.431