Issue 4
Journal for Veterinary Medicine, Biotechnology and Biosafety
Volume
1, Issue 4, December 2015, Pages 28–34
ISSN 2411-3174 (print version) ISSN 2411-0388
(online version)
Cellular and humoral mediated immunity and distribution of viral
antigens in chickens after infection with a low pathogenic avian influenza
virus (LPAIV H4N6) isolated
from wild ducks
Stegniy B. T. 1, Muzyka
D. V. 1, Shutchenko P. O. 1, Lillehoj H. 2, Kovalenko
L. V. 1, Stegniy A. B. 1, Medvid K. O. 1, Rula
O. M. 1
1 National Scientific Center ‘Institute of Experimental and
Clinical Veterinary Medicine’, Kharkiv,
Ukraine, e-mail: dmuzyka77@gmail.com
2 Animal Biosciences and Biotechnology
Laboratory, Beltsville Agricultural Research Center, Beltsville, Maryland, USA
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PDF (print version)
Citation for
print version: Stegniy, B. T., Muzyka, D. V., Shutchenko, P. O., Lillehoj, H., Kovalenko, L. V., Stegniy, A. B.,
Medvid, K. O. and Rula, O.
M. (2015) ‘Cellular and humoral mediated
immunity and distribution of viral antigens in chickens after infection with a
low pathogenic avian influenza virus (LPAIV H4N6) isolated from wild ducks’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 1(4), pp. 28–34.
Download
PDF (online version)
Citation for
online version: Stegniy, B. T., Muzyka, D. V., Shutchenko, P. O., Lillehoj, H., Kovalenko, L. V., Stegniy, A. B.,
Medvid, K. O. and Rula, O.
M. (2015) ‘Cellular and humoral mediated
immunity and distribution of viral antigens in chickens after infection with a
low pathogenic avian influenza virus (LPAIV H4N6) isolated from wild ducks’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 1(4),
pp. 28–34. Available at: http://jvmbbs.kharkov.ua/archive/2015/volume1/issue4/oJVMBBS_2015014_028-034.pdf
Summary. Four-week-old commercial chickens were intranasally inoculated with
a H4N6 low pathogenic avian influenza virus (LPAIV) isolated from a garganey
in Ukraine. The virus did not cause disease in chickens and no pathological
changes were observed in inoculated birds. No virus
was isolated from internal organs, but 3 chickens of 5
showed antibodies to influenza virus at intranasal infection. Cecum, spleen, lung, and trachea samples were collected
from LPAIV-infected chickens from 1
to 14 days post inoculation (dpi) and examined by immunohistochemical
techniques to determine the distribution of LPAIV and
evaluate the host immune response using various immune markers. From 7 to 14 dpi, a sharp increase of the number of cells bearing
CD4, IgM, IgG, and IgA was observed. In
spleen, the number of CD4 T lymphocytes and
macrophages were increased in immunohistochemical
staining when compared to controls. In the lung, B
lymphocytes expressing IgM (6.8±0.5%), IgG (9.4±1.3%), and IgA
(8.6±0.1%) were detected in higher numbers than in controls. High levels
of IFN-γ, IL-2, IL-15
were present on 7 dpi. We also found LPAIV
nucleoprotein (NP) staining in the trachea observed on 10 dpi (2.7±0.4% of antibody-stained areas) as well as in the spleen on
5 dpi (3.3±0.2%). There was no NP antigen in other organs. In
conclusion, although infection with a LPAIV did not cause obvious clinical disease, viral replication was seen
in the trachea and spleen and both local and systemic cellular and humoral immune responses were elicited in these LPAIV-infected chickens.
Keywords: avian
influenza, low pathogenic avian influenza virus, cellular immunity, humoral immunity, viral antigen, wild birds
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