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Issue 3

Journal for Veterinary Medicine, Biotechnology and Biosafety

Volume 2, Issue 3, October 2016, Pages 5–11

ISSN 2411-3174 (print version) ISSN 2411-0388 (online version)

SNP L127V OF GROWTH HORMONE GENE IN BREEDING HERD OF ABERDEEN ANGUS IN KHARKIV REGION, EASTERN UKRAINE

¹ Fedota O. M., ² Ruban S. Yu., ³ Lysenko N. G. ^* , ⁴ Kolisnyk A. I., ⁵ Goraichuk I. V., ⁶ Tyzhnenko T. V.

¹ V. N. Karazin Kharkiv National University, Kharkiv, Ukraine, e-mail: afedota@mail.ru

² Institute of Animal Breeding and Genetics nd. a. M. V. Zubets of NAAS, Chubynske, Ukraine

³ V. N. Karazin Kharkiv National University, Kharkiv, Ukraine

⁴ PE ‘Agrofirma Svitanok’, Novoselivka, Ukraine

⁵ National Scientific Center ‘Institute of Experimental and Clinical Veterinary Medicine’, Kharkiv, Ukraine

⁶ V. N. Karazin Kharkiv National University, Kharkiv, Ukraine

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Citation for print version: Fedota, O. M., Ruban, S. Yu., Lysenko, N. G., Kolisnyk, A. I., Goraichuk, I. V. and Tyzhnenko, T. V. (2016) ‘SNP L127V of growth hormone gene in breeding herd of Aberdeen Angus in Kharkiv region, Eastern Ukraine’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 2(3), pp. 5–11.

Download PDF (online version)

Citation for online version: Fedota, O. M., Ruban, S. Yu., Lysenko, N. G., Kolisnyk, A. I., Goraichuk, I. V. and Tyzhnenko, T. V. (2016) ‘SNP L127V of growth hormone gene in breeding herd of Aberdeen Angus in Kharkiv region, Eastern Ukraine’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 2(3), pp. 5–11. Available at: http://jvmbbs.kharkov.ua/archive/2016/volume2/issue3/oJVMBBS_2016023_005-011.pdf

Summary. Bovine growth hormone (bGH) effects on animal growth and metabolism. Therefore, it plays a vital role in regulation of body weight, fertility and lactation performance of cattle. This enables bGH gene to be used as a candidate marker for improving growth, meat or milk production and for marked-assisted selection programs of cattle too. The aim of this study is analyzing the polymorphic variant L127V of bGH gene in Aberdeen-Angus bred in Kharkiv region and comparing of obtained result with commercial herds from other countries. PCR-RFLP methods were set up for the SNP genotyping. Testing deviation from the Hardy-Weinberg equilibrium was performed using Pearson’s chi-squared test. Pearson’s correlation coefficient r was used to measure the strength of association between two characteristics. The allele and genotype frequencies of SNP L127V (rs41923484; g.2141C>G) are: L — 0.319 and V — 0.681; LL — 8.6%, LV — 46.6% and VV — 44.8% (n=58), population is in Hardy-Weinberg equilibrium. The studied population is close to beef herds of Russian selection. It was found a positive correlation of L‑allele with birth weight within the moderate climatic zone (r=0.93).

Keywords: Aberdeen-Angus breed, growth hormone gene, SNP L127V

References:

Atramentova, L. A. and Utevskaya, A. M. (2008) Statistical methods in biology [Statisticheskie metody v biologii]. Gorlovka: Lіkhtar. ISBN 9789662129267. [in Russian]

Balogh, O., Kovács, K., Kulcsár, M., Gáspárdy, A., Zsolnai, A., Kátai, L., Pécsi, A., Fésüs, L., Butler, W. R. and Huszenicza, G. (2009) ‘AluI polymorphism of the bovine growth hormone (GH) gene, resumption of ovarian cyclicity, milk production and loss of body condition at the onset of lactation in dairy cows’, Theriogenology, 71(4), pp. 553–661. http://dx.doi.org/10.1016/j.theriogenology.2008.06.032

Beyshova, I. S., Nametov, A. M. and Terletskiy, V. P. (2016) ‘Development of genetic markers for productivity traits in beef pedigree cattle of Auliekol and Kazakh white head breeds’ [Razrabotka geneticheskikh markerov dlya priznakov myasnoy produktivnosti plemennogo krupnogo rogatogo skota Auliekol'skoy i Kazakhskoy belogolovoy porod], Veterinary, Zootechnics and Biotechnology [Veterinariya, zootekhniya i biotekhnologiya], 1, pp. 36–42. Available at: http://elibrary.ru/download/71223733.htm. [in Russian]

Bochkov, V. M., Luniova, A. E., Tarasiuk, S. I. and Nasyrova, I. A. (2009) ‘Genetic structure of Volynian beef breed of cattle by the somatostatin polymorphic variant’ [Henetychna struktura za polimorfizmom somatotropnoho hormonu volynskoi m’iasnoi porody velykoi rohatoi khudoby], Scientific Bulletin of National University of Life and Environmental Sciences [Naukovyi visnyk Natsionalnoho universytetu bioresursiv i pryrodokorystuvannia Ukrainy], 138, pp. 332–336. Available at: http://www.nbuv.gov.ua/old_jrn/Chem_Biol/nvnau/2009_138/09bvm.pdf. [in Ukrainian]

Chikuni, K., Terada, F., Kageyama, S., Koishikawa, T., Kato, S. and Ozutsumi, K. (1991) ‘Identification of DNA sequence variants for amino acid residues 127 of bovine growth hormone using the polymerase chain reaction method’, Animal Science and Technology [Nihon Chikusan Gakkaiho], 62(7), pp. 660–666. http://dx.doi.org/10.2508/chikusan.62.660. [in Japanese]

Dario, C., Carnicella, D., Ciotola, F., Peretti, V. and Bufano G. (2008) ‘Polymorphism of growth hormone GH1-AluI in Jersey Cows and its effect on milk yield and composition’, Asian-Australasian Journal of Animal Sciences, 21(1), pp. 1–5. http://dx.doi.org/10.5713/ajas.2008.60586

Ge, W., Davis, M. E., Hines, H. C., Irvin, K. M. and Simmen, R. C. M. (2003) ‘Association of single nucleotide polymorphisms in the growth hormone and growth hormone receptor genes with blood serum insulin-like growth factor I concentration and growth traits in Angus cattle’, Journal of Animal Science, 81(3), pp. 641–648. http://dx.doi.org/10.2527/2003.813641x

Gill, J. L., Bishop, S. C., McCorquodale, C., Williams, J. L. and Wiener, P. (2009) ‘Association of selected SNP with carcass and taste panel assessed meat quality traits in a commercial population of Aberdeen Angus-sired beef cattle’, Genetics Selection Evolution, 41(1), pp. 36. http://dx.doi.org/10.1186/1297-9686-41-36

Hadi, Z., Atashi, H., Dadpasand, M., Derakhshandeh, A. and Ghahramani Seno, M. M. (2015) ‘The relationship between growth hormone polymorphism and growth hormone receptor genes with milk yield and reproductive performance in Holstein dairy cows’, Iranian Journal of Veterinary Research, 16(3), pp. 244–248. Available at: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4782692/pdf/ijvr-16-244.pdf

Hartatik, T., Volkandari, S. D., Rachman, M. P. and Sumadi (2013) ‘Polymorphism leu/val of growth hormone gene identified from limousin cross local cattle in Indonesia’, Procedia Environmental Sciences, 17, pp. 105–108. http://dx.doi.org/10.1016/j.proenv.2013.02.017

Hradecká, E., Čítek, J., Panicke, L., Řehout, V. and Hanusová, L. (2008) ‘The relation of GH1, GHR and DGAT1 polymorphisms with estimated breeding values for milk production traits of German Holstein sires’, Czech Journal of Animal Science, 53(6), pp. 238–245. Available at: http://www.agriculturejournals.cz/publicFiles/01545.pdf

Hyl, M. I., Horodna, O. V., Kramarenko, S. S. and Smetana, O. Yu. (2011) ‘Analysis of cow milk production dependence on polymorphic variants of separate structure genes’ [Analiz zalezhnosti molochnoi produktyvnosti koriv vid polimorfizmu okremykh strukturnykh heniv], Scientific Bulletin of National University of Life and Environmental Sciences [Naukovyi visnyk Natsionalnoho universytetu bioresursiv i pryrodokorystuvannia Ukrainy], 160(2), pp. 285–293. Available at: http://www.nbuv.gov.ua/old_jrn/Chem_Biol/nvnau_tvppt/2011_160_2/11gmi.pdf. [in Ukrainian]

Kemenes, P. A., Regitano, L. C. de A., Rosa, A. J. de M., Packer, I. U., Razook, A. G., Figueiredo, L. A. de, Silva, N. A., Etchegaray, M. A. L. and Coutinho, L. L. (1999) ‘k‑Casein, b‑lactoglobulin and growth hormone allele frequencies and genetic distances in Nelore, Gyr, Guzerá, Caracu, Charolais, Canchim and Santa Gertrudis cattle’, Genetics and Molecular Biology, 22(4), pp. 539–541. http://dx.doi.org/10.1590/s1415-47571999000400012

Komisarek, J., Michalak, A. and Walendowska, A. (2011) ‘The effects of polymorphisms in DGAT1, GH and GHR genes on reproduction and production traits in Jersey cows’, Animal Science Papers and Reports, 29(1), pp. 29–36. Available at: http://archiwum.ighz.edu.pl/files/objects/7501/66/strona29-36.pdf

Kopylova, K. V., Kopylov, K. V. and Arnaut, K. O. (2009) ‘Genetic structure characteristics of cattle by Quantitative traits loci (QTL)’ [Osoblyvosti henetychnoi struktury riznykh porid velykoi rohatoi khudoby za lokusamy kilkisnykh oznak (QTL)], Scientific Bulletin of National University of Life and Environmental Sciences [Naukovyi visnyk Natsionalnoho universytetu bioresursiv i pryrodokorystuvannia Ukrainy], 138, pp. 239–245. Available at: http://www.nbuv.gov.ua/old_jrn/Chem_Biol/nvnau/2009_138/09kkv.pdf. [in Ukrainian]

Kostenko, S. O. and Starodub, L. F. (2011) ‘Prediction of beef breeds bulls productivity based on cytogenetic and molecular genetic markers’ [Prohnoz produktyvnosti buhaiv miasnykh porid na osnovi tsytohenetychnykh ta molekuliarno-henetychnykh markeriv], Scientific Bulletin of National University of Life and Environmental Sciences [Naukovyi visnyk Natsionalnoho universytetu bioresursiv i pryrodokorystuvannia Ukrainy], 160(2), pp. 266–273. Available at: http://www.nbuv.gov.ua/old_jrn/Chem_Biol/nvnau_tvppt/2011_160_2/11slv.pdf. [in Ukrainian]

Krasnopiorova, N., Baltrenaite, L. and Miceikiene, I. (2012) ‘Growth hormone gene polymorphism and its influence on milk traits in cattle bred in Lithuania’ Veterinarija ir Zootechnika, 58, pp. 42–46. Available at: http://vetzoo.lsmuni.lt/data/vols/2012/58/pdf/krasnopiorova.pdf

Lee, J.‑H., Lee, Y.‑M., Oh, D.‑Y., Jeong, D.‑J. and Kim, J.‑J. (2013) ‘Identification of single nucleotide polymorphisms (SNPs) of the bovine growth hormone (bGH) gene associated with growth and carcass traits in Hanwoo’, Asian-Australasian Journal of Animal Sciences, 26(10), pp. 1359–1364. http://dx.doi.org/10.5713/ajas.2013.13248

Mokhnachova, N., Suprovich, T., Dobrynska, M. and Fursa, N. (2016) ‘Characteristics of Ukrainian Grey cattle by DNA-markers’ [Kharakterystyka Siroi ukrainskoi porody velykoi rohatoi khudoby za DNK-markerany], Animal Breeding and Genetics [Rozvedennia i henetyka tvaryn], 51, pp. 283–289. Available at: http://digest.iabg.org.ua/images/digest/51/Animal_breeding_and_genetics_51.pdf. [in Ukrainian]

Nei, M. (1972) ‘Genetic distance between populations’, The American Naturalist, 106(949), pp. 283–292. http://dx.doi.org/10.1086/282771

Nekrasov, A. A., Popov, A. N., Popov, N. A. and Fedotova, E. G. (2016) ‘Impacts of milk protein and hormone gene polymorphisms on energy for growth of Holstein Black-and-White heifers’ [Vliyanie polimorphizma genov molochnykh belkov i gormonov na energiyu rosta telok Cherno-pestroy Golshtinskoy porody], Tavric Scientific Review [Tavricheskiy nauchnyy obozrevatel'], 5(2), pp. 91–95. Available at: http://elibrary.ru/download/12725655.pdf. [in Russian]

Podoba, B. Ye., Arnaut, K. O., Kovtun, S. I. and Shcherbak, O. V. (2009) ‘Study of Ukrainian Grey breed by genetic markers and embryotechnological approaches’ [Doslidzhennia henofondu siroi ukrainskoi porody za henetychnymy markeramy ta embriotekhnolohichnymy pidkhodamy], Scientific Bulletin of National University of Life and Environmental Sciences [Naukovyi visnyk Natsionalnoho universytetu bioresursiv i pryrodokorystuvannia Ukrainy], 138, pp. 234–239. Available at: http://www.nbuv.gov.ua/old_jrn/Chem_Biol/nvnau/2009_138/09pbe.pdf. [in Ukrainian]

Regitano, L. C. A., Azevedo, J. L., Vencovsky, R., Packer, I. U., Barbosa, P. F., Rosa, A. J. M., Silva, N. A., Etchegaray, M. A. L. and Coutinho, L. L. (1999) ‘Selection for breed-specific growth hormone and IGF-I alleles in a synthetic beef cattle cross, Canchim’, Genetics and Molecular Biology, 22(4), pp. 531–537. http://dx.doi.org/10.1590/s1415-47571999000400011.

Salces A. J., Icalia, P. J. C., Mendioro, M. S. and Sevilla, C. C. (2014) ‘SNP analysis of the growth hormone gene in indigenous Philippine cattle, Ilocos genetic group by PCR-RFLP’, Proceedings of the 10^th World Congress on Genetics Applied to Livestock Production, 17–22 August, 2014, Vancouver, BC, Canada, pp. 515. Available at: https://asas.confex.com/asas/WCGALP14/webprogram/Paper10015.html.

Sulimova, G. E., Fedunin, A. A., Klimov, E. A. and Stolpovski, Yu. A. (2011) ‘Evaluation of cattle genetic potential of for traits of good quality meat on the basis of DNA-markers’ [Otsenka geneticheskogo potentsiala otechestvennogo skota po priznakam vysokogo kachestva myasa na osnove DNK-markernykh sistem], Problems of productive animal biology [Problemy biologii produktivnykh zhivotnykh], 1, pp. 62–64. Available at: http://elibrary.ru/item.asp?id=16092146. [in Russian]

Thomas, M. G., Enns, R. M., Shirley, K. L., Garcia, M. D., Garrett, A. J. and Silver, G. A. (2007) ‘Associations of DNA polymorphisms in growth hormone and its transcriptional regulators with growth and carcass traits in two populations of Brangus bulls’, Genetics and Molecular Research, 6(1), pp. 222–237. Available at: http://www.funpecrp.com.br/gmr/year2007/vol1-6/pdf/gmr0277.pdf.

Vasconcellos, L. P. de M. K., Tambasco-Talhari, D., Pereira, A. P., Coutinho, L. L. and Regitano, L. C. de A. (2003) ‘Genetic characterization of Aberdeen Angus cattle using molecular markers’, Genetics and Molecular Biology, 26(2), pp. 133–137. http://dx.doi.org/10.1590/s1415-47572003000200005.