Issue 1
Journal for Veterinary Medicine, Biotechnology and Biosafety
Volume
4, Issue 1, March 2018, Pages 15–17
ISSN 2411-3174 (print version) ISSN 2411-0388
(online version)
STUDY OF THE INFLUENCE OF
BACTERICIDAL PREPARATION ON CELL CULTURES
Kovalenko V. L. 1, Harkavenko V. M. 1, Kukhtyn M. D. 2, Ponomarenko G. V. 3, Ihnatieva T. M. 3
1 State Scientific Control Institute of
Biotechnology and Strains of Microorganisms,
Kyiv, Ukraine, e-mail: kovalenkodoktor@gmail.com
2 Ivan Puluj Ternopil National Technical University, Ternopil,
Ukraine; е-mail: kuchtyn@yandex.ua
3 Kharkiv State Zooveterinary Academy, Kharkiv,
Ukraine, e-mail: gpkh1966@gmail.com
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PDF (print version)
Citation for print version: Kovalenko, V. L.,
Harkavenko, V. M., Kukhtyn, M. D.,
Ponomarenko, G. V. and Ihnatieva, T. M. (2018) ‘Study of the
influence of bactericidal preparation on cell cultures’, Journal for Veterinary Medicine, Biotechnology and
Biosafety, 4(1), pp. 15–17.
Download
PDF (online version)
Citation for online version: Kovalenko, V. L.,
Harkavenko, V. M., Kukhtyn, M. D.,
Ponomarenko, G. V. and Ihnatieva, T. M. (2018) ‘Study of the
influence of bactericidal preparation on cell cultures’, Journal for Veterinary Medicine, Biotechnology and
Biosafety. [Online] 4(1), pp. 15–17. Available at:
http://jvmbbs.kharkov.ua/archive/2018/volume4/issue1/oJVMBBS_2018041_015-014.pdf
Summary. Pressing problem today is the existence of a
large number of microorganisms — causative agents of viral diseases,
highly resistant to bactericidal preparations. At the same time, an important
direction of research is the study of the toxic effects of new disinfectants
not only on the organism of laboratory animals, but also on cell cultures,
which is more humane and meets the modern standards of bioethics. That’s
what determined the purpose of research — the study of the antiviral
and toxic effects of the bactericidal preparation ‘Barez’
on the Aujeszky’s disease virus strain
‘Clone-B’, tested and adapted to the continuous cell cultures SNEV and PTP. When conducting
research there were studied methods for assessing toxic effects of
antibacterial drug ‘Barez’ on the cell
cultures of swine origin SNEV, PTP,
and its effect on the titer of infectious activity of the vaccine strain
‘Clone-B’ of Aujeszky’s disease
virus from the collection of strains of the State Scientific Control Institute
of Biotechnology and Strains of Microorganisms. When studying the antiviral
properties of the bactericidal preparation on the Aujeszky’s
disease virus ‘Clone-B’ strain, tested and adapted for continuous
cell cultures SNEV and PTP,
there was determined that 0.2–0.5% solutions of the drug ‘Barez’ are not toxic when effecting cell cultures,
but exhibit inactivating effect on the Aujeszky’s
disease virus. Therefore, it is possible to use it for preventive disinfection
of objects of veterinary medicine in the presence of animals in recommended
concentrations and exposures.
Keywords: bactericidal drug ‘Barez’,
cell cultures, toxicity, Aujeszky’s disease
virus, silver nanoparticles, benzalkonium chloride,
essential oils
References:
Ababutain, I. M.
(2011) ‘Antimicrobial activity of ethanolic
extracts from some medicinal plant’, Australian
Journal of Basic and Applied Sciences, 5(11), pp. 678–683.
Available at: http://www.ajbasweb.com/old/ajbas/2011/November-2011/678-683.pdf
Deyneka, S. E.
(1999) ‘Comparative express in
vitro assessment of the degree of toxicity and danger of a number of metal
salts’ [Sravnitel’naya ekspress-otsenka in
vitro stepeni toksichnosti
i opasnosti ryada soley metallov],
Hygiene of Inhabited Places [Gigiena naselennykh mest], 35, pp. 549–555. [in
Russian]
Kovalenko, V. L,
Belokon’, V. I., Lopatko, K. G.
and Ponomarenko, G. V. (2010)
‘Assessment microbicide toxicity on cell cultures origin pork SNEK, PTP’ [Otsinka toksychnosti bakterytsydnykh zasobiv na klitynnykh
kulturakh svyniachoho pokhodzhennia SNEV, PTP], Problems of Zooengineering and Veterinary Medicine [Problemy
zooinzhenerii ta veterynarnoi
medytsyny], 22(2.1), pp. 114–118. [in Ukrainian]
Kovalenko, V. L. and Nedosiekov, V. V.
(2011) Methodical approaches to the
control of disinfectants for veterinary medicine [Metodychni
pidkhody shchodo kontroliu dezinfikuiuchykh zasobiv dlia veterynarnoi
medytsyny]. Kyiv. [in Ukrainian]
Pathmanathan, M. K., Uthayarasa, K., Jeyadevan, J. P. and Jeyaseelan, E. C.
(2010) ‘In vitro antibacterial
activity and phytochemical analysis of some selected medicinal plants’, International Journal of Pharmaceutical and
Biological Archives, 1(3), pp. 291–299. Available at: http://ijpba.info/ijpba/index.php/ijpba/article/view/138
Stadnytska, N. Ye.,
Komarovska-Porokhniavets, O. Z., Kishchak, Kh. Ya., Mykoliv, O. B., Lytvyn, B. Ya., Konechna, R. T. and Novikov, V. P.
(2011) ‘Plants with antimicrobial properties’ [Roslyny
z protymikrobnymy vlastyvostiamy],
Herald of the Lviv
Polytechnic National University. Chemistry, Technology Substances and Their Use
[Visnyk Natsionalnoho universytetu ‘Lvivska politekhnika. Khimiia, tekhnolohiia rechovyn ta yikh zastosuvannia], 700,
pp. 111–116. Available at: http://ena.lp.edu.ua/bitstream/ntb/10547/1/28.pdf. [in Ukrainian]