Issue 1
Journal for Veterinary Medicine, Biotechnology and Biosafety
Volume
4, Issue 1, March 2018, Pages 24–27
ISSN 2411-3174 (print version) ISSN 2411-0388
(online version)
EFFECTS OF MTHFR GENE ON REPRODUCTIVE HEALTH AND PRODUCTIVE TRAITS OF
DAIRY COWS
Fedota O. M. 1,
Skrypkina I. Y. 2, Mitioglo L. V. 3,
Tyzhnenko T. V. 1, Ruban S. Yu. 4
1 V. N. Karazin
Kharkiv National University, Kharkiv,
Ukraine, e-mail: amsfedota@gmail.com
2 Institute of Molecular Biology and Genetics of
the National Academy of Sciences of Ukraine, Kyiv, Ukraine
3 SE RF ‘Nyva’, Khrystynivka,
Ukraine
4 National
University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine
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PDF (print version)
Citation for print version: Fedota, O. M., Skrypkina, I. Y., Mitioglo, L. V., Tyzhnenko, T. V.
and Ruban, S. Yu. (2018) ‘Effects of MTHFR gene on reproductive health and productive traits of
dairy cows’, Journal
for Veterinary Medicine, Biotechnology and Biosafety, 4(1),
pp. 24–27.
Download
PDF (online version)
Citation for online version: Fedota, O. M., Skrypkina, I. Y., Mitioglo, L. V., Tyzhnenko, T. V.
and Ruban, S. Yu. (2018) ‘Effects of MTHFR gene on reproductive health and productive traits of
dairy cows’, Journal
for Veterinary Medicine, Biotechnology and Biosafety.
[Online] 4(1), pp. 24–27. Available at: http://jvmbbs.kharkov.ua/archive/2018/volume4/issue1/oJVMBBS_2018041_024-027.pdf
Summary. One-carbon metabolism of mammals is one of
the key points of metabolism and the pleiotropic effects of human MTHFR gene determining reproductive health are known. The aim of this study was an evaluation the role
of MTHFR gene in lactating dairy cows. Cows were genotyped by sequencing. The plasma homocysteine level, bone mineral density, traditional
production and reproduction traits were analyzed.
Statistical methods included Pearson’s chi-squared and t criteria, Pearson’s and
Spearmen correlation coefficient r
and ANOVA. Allele frequencies of SNP 8137C/T of MTHFR gene were: 0.943 (C) and 0.057 (T). Distribution of genotypes was
88.6% (CC) : 11.4% (CT) :
0% (TT). Investigated group of animals was in
Hardy-Weinberg equilibrium. We had founded that calving interval were shorter
in cows with the CC genotype than in CT animals, 378.6 vs 405.9 days, and than in the herd as a whole — 378.6 vs 388.5
days. Lactation period of CC cows shorter by 10 days than in CT cows,
321.7 vs 331.5 days. Analysis of traits of CC cows and animals in the herd
had demonstrated that the age of first insemination and the age of first
calving are significantly lower in CC cows than in the herd as a whole, 525.8 ± 17.8 vs
642.9 ± 7.5 days, and 808.6 ± 18.5 vs
936.6 ± 8.0 days. Higher bone mineral density values as an indicator of body health are observed in CC cows compared with CT animals, 3,580.3 vs
3,359.0 mg/mm3. The study of MTHFR gene associated with
reproductive traits in cows is relevant as a basis for breeding and biochemical
correction of gene effects causing the reproductive disorders of animals.
Keywords: dairy cows, MTHFR
gene, homocysteine, bone mineral density, milk
production, reproduction of cows
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