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Issue 2

Journal for Veterinary Medicine, Biotechnology and Biosafety

Volume 4, Issue 2, June 2018, Pages 17–24

ISSN 2411-3174 (print version) ISSN 2411-0388 (online version)

EFFECT OF PHYTONUTRIENT ‘VITASTIM’ ON CHICKEN MUCOSAL IMMUNITY AGAINST LOW PATHOGENIC AVIAN INFLUENZA VIRUS H4N6

Kovalenko L. V. ¹, Solodiankin O. S. ¹, Stegniy B. T. ¹, Gerilovych A. P. ¹, Muzyka D. V. ¹, Stegniy M. Yu. ¹, Obukhovska O. V. ¹, Shutchenko P. O. ¹, Lillehoj H. S. ²

¹ National Scientific Center ‘Institute of Experimental and Clinical Veterinary Medicine’,
Kharkiv, Ukraine, e-mail: larbuko@gmail.com

² Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Beltsville
Agricultural Research Center, Beltsville, Maryland, USA, e-mail: hyun.lillehoj@ars.usda.gov

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Citation for print version: Kovalenko, L. V., Solodiankin, O. S., Stegniy, B. T., Gerilovych, A. P., Muzyka, D. V., Stegniy, M. Yu., Obukhovska, O. V., Shutchenko, P. O. and Lillehoj, H. S. (2018) ‘Effect of phytonutrient ‘Vitastim’ on chicken mucosal immunity against Low pathogenic avian influenza virus H4N6’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 4(2), pp. 17–24.

Download PDF (online version)

Citation for online version: Kovalenko, L. V., Solodiankin, O. S., Stegniy, B. T., Gerilovych, A. P., Muzyka, D. V., Stegniy, M. Yu., Obukhovska, O. V., Shutchenko, P. O. and Lillehoj, H. S. (2018) ‘Effect of phytonutrient ‘Vitastim’ on chicken mucosal immunity against Low pathogenic avian influenza virus H4N6’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 4(2), pp. 17–24. Available at: http://jvmbbs.kharkov.ua/archive/2018/volume4/issue2/oJVMBBS_2018042_017-024.pdf

Summary. The study of immunostimulating phytogenous preparation ‘Vitastim’ influence on chickens infected by subtype H4N6 avian influenza virus was provided. It was determined, that preparation usage is more actively influences on humoral mediators of immune reaction and proliferation activity of spleen lymphocytes under conditions of low pathogenic avian influenza. This is evidenced by an increase in proliferative activity of chicken splenocytes at 5–14 days and an increase in production of NO during 14 days of the experiment. According to the quantitative real-time PCR results the expression of IL‑2, IL‑13, IL‑17α in peripheral blood was effectively increasing with the promotion of enhances anti-viral activity. Due to the immunohistochemical studies of chicken it was found the influence of the phytonutrient ‘Vitastim’ on humoral immune system by registration of high levels of IgM, IgG, IgA. Cell immunity was stimulated by the ‘Vitastim’, however, as evidenced by the low levels of CD4, macrophages, IL‑2, IL‑15 in chickens of experimental groups compared to the intact birds, cell immunity does not play a significant role in the pathogenesis of LPAI. The phytonutrient ‘Vitastim’ produces moderated effect on the cell immune system at influenza virus infection with stimulation of IL‑2, IL‑4, IL‑17, IFN‑γ expression with irregular tendency in different observation periods.

Keywords: low pathogenic avian influenza virus, H4N6, chickens, vitastim, phytonutrient, mucosal immunity

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