• Home
• Archive
• Rules for Authors
• Policies on Conflict of Interest, Human and animal rights, and Informed Consent
• Peer reviewering policy
• Editorial Board
• Certificate
• Editorial Ethics
• Contacts

Issue 3

Journal for Veterinary Medicine, Biotechnology and Biosafety

Volume 4, Issue 3, September 2018, Pages 17–19

ISSN 2411-3174 (print version) ISSN 2411-0388 (online version)

ELISA DIAGNOSTIC OF METAPNEUMOVIRUS AND REOVIRUS INFECTIONS IN POULTRY

Ivleva O. V. ¹, Nalyvaiko L. I. ¹, Ryabinin S. V. ²

¹ Luhansk National Agrarian University, Kharkiv, Ukraine, e-mail: sauce1908@gmail.com

² State Research Station of Poultry Industry of the National Academy of Agrarian Sciences of Ukraine, Kharkiv, Ukraine, e-mail: ryabinin.sv1979@gmail.com

Download PDF (print version)

Citation for print version: Ivleva, O. V., Nalyvaiko, L. I. and Ryabinin, S. V. (2018) ‘ELISA diagnostic of Metapneumovirus and Reovirus infections in poultry’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 4(3), pp. 17–19.

Download PDF (online version)

Citation for online version: Ivleva, O. V., Nalyvaiko, L. I. and Ryabinin, S. V. (2018) ‘ELISA diagnostic of Metapneumovirus and Reovirus infections in poultry’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 4(3), pp. 17–19. Available at: http://jvmbbs.kharkov.ua/archive/2018/volume4/issue3/oJVMBBS_2018043_017-019.pdf

Summary. The import of high-yielding poultry breeds has caused the proliferation of viral diseases which previously did not occur at Ukrainian poultry enterprises, such as avian metapneumovirus (AMPV) and avian reovirus (ARV) infections. Most pathogens cause immunosuppression in poultry (including ARV infection) and are frequently diagnosed as co‑infections with other infectious agents which are capable of causing pathological changes in poultry (especially if aided by stress). AMPV and ARV co‑infection cannot be excluded; these diseases are manifested in respiratory and intestinal disorders, their subclinical, latent, and associated forms present a challenge for laboratory diagnostic as well as for the implementation of prophylactic measures. The objective of the present work was to conduct the epizootological survey of AMPV and ARV infections incidence at poultry enterprises of different forms of property and to produce an antigen suitable for the development of an ELISA test-system for the determination of antibodies against AMPV in blood serums of turkeys and hens. The pathological material used in the experiments (heads, tracheas, and lungs from ill or deceased poultry) for the isolation of pneumovirus was collected from 60‑day‑old turkeys in 2009. The pathological material for virology research was preserved at – 20 °С. Serology and virology research was conducted using standard methods. AMPV and ARV incidence monitoring was conducted at poultry enterprises of Kharkiv region among turkeys of ‘Big‑6’ and ‘Big‑8’ breeds imported from Germany and Hungary (aged 4–480 days), among turkeys of ‘Large White’ breed (aged 70 days), and hens of ‘Birkivska Barvysta’ and ‘Hisex Brown’ (aged 250 and 180 days correspondingly). On average, 78.0% of the examined turkeys with antibody titers 1:3,776–1:27,869, 100% of the ‘Birkivska Barvysta” hens with antibody titers 1:4,997–1:10,414 and 0% of the ‘Hisex Brown’ hens were AMPV‑positive. No hens were ARV‑positive. At the Luhansk National Agrarian University (Kharkiv) the development of a domestic ELISA test-system for the diagnostic of AMPV in turkeys and hens is carried out.

Keywords: epizootiology, metapneumovirus, reovirus, infection, poultry, purification and concentration, ELISA

References:

Abdil’aziz, F. (1990) ‘Study of the biological properties of reoviruses isolated from chickens and turkeys’ [Izuchenie biologicheskikh svoystv reovirusov, vydelennykh ot kur i indeek], Veterinary Medicine [Veterinariya: respublikanskiy mezhvedomstvennyy tematicheskiy nauchnyy sbornik], 65, pp. 104–107. [in Russian]

Aliev, A. S. (2005) ‘Avian reovirus infection’ [Reovirusnaya infektsiya ptits], Livestock Veterinary Medicine [Veterinariya sel’skokhozyaystvennykh zhivotnykh], 12, pp. 28–32. [in Russian]

Borisova, I. A. and Starov, S. K. (2006) ‘Avian pneumovirus infection’ [Pnevmovirusnaya infektsiya ptits]. Proceedings of the Federal Centre for Animal Health [Trudy Federal’nogo tsentra okhrany zdorov’ya zhivotnykh], 4, pp. 281–296. Available at: https://elibrary.ru/item.asp?id=14454020. [in Russian]

German, I. V. and German, V. V. (2002) ‘The study of the virulent properties of avireoviruses isolated from laying hens, broilers and goslings’ [Vyvchennia virulentnykh vlastyvostei avireovirusiv, izolovanykh vid kurei-nesuchok, broileriv ta huseniat], Veterinary Medicine of Ukraine [Veterynarna medytsyna Ukrainy], 7, pp. 37–38. [in Ukrainian]

Nalyvaiko, L. I., Bezrukava, I. Yu., Nikolaenko, Yu. Yu., Bondarenko, A. L. and Riabeka, D. A. (2011) ‘Epizootiological monitoring of avian metapneumovirus infection in poultry farms in Ukraine’ [Epizootolohichnyi monitorynh metapnevmovirusnoi infektsii u ptakhivnychykh hospodarstvakh Ukrainy], Veterinary Medicine of Ukraine [Veterynarna medytsyna Ukrainy], 1, pp. 9–11. [in Ukrainian]

Reed, L. J. and Muench, H. (1938) ‘A simple method of estimating fifty per cent endpoints’, American Journal of Hygiene, 27(3), pp. 493–497. Available at: http://aje.oxfordjournals.org/content/27/3/493.full.pdf

Riabinin, S. V., Nikolaenko, Yu. Yu. and Nalyvaiko, L. I. (2012) ‘Tenosynovitis of hens: diagnostic and prevention’ [Tenosynovit kurei: diahnostyka ta profilaktyka], Veterinary Medicine of Ukraine [Veterynarna medytsyna Ukrainy], 10, pp. 17–20. [in Ukrainian]