Issue 3
Journal for Veterinary Medicine, Biotechnology and Biosafety
Volume
4, Issue 3, September 2018, Page 31
ISSN 2411-3174 (print version) ISSN 2411-0388
(online version)
CIRCULATION OF INFLUENZA A
VIRUS AMONG WILD BIRDS IN KAZAKHSTAN
Sultankulova K. T., Akylbayeva K. K., Kerimbayev A.,
Burashev E. D., Orynbayev M. B.
Research Institute for Biological Safety Problems of
the Science Committee of the Ministry of Education and Science of the Republic
of Kazakhstan, Gvardeyskiy, Korday
District, Jambyl Region, Republic of Kazakhstan,
e-mail: sultankul70@mail.ru
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PDF (print version)
Citation for print version: Sultankulova, K. T.,
Akylbayeva, K. K., Kerimbayev, A.,
Burashev, E. D. and Orynbayev, M. B.
(2018) ‘Circulation of influenza A virus among wild birds in Kazakhstan’,
Journal for Veterinary Medicine,
Biotechnology and Biosafety, 4(3), p. 31.
Download
PDF (online version)
Citation for online version: Sultankulova, K. T.,
Akylbayeva, K. K., Kerimbayev, A.,
Burashev, E. D. and Orynbayev, M. B.
(2018) ‘Circulation of influenza A virus among wild birds in Kazakhstan’,
Journal for Veterinary Medicine,
Biotechnology and Biosafety. [Online] 4(3), p. 31. Available at:
http://jvmbbs.kharkov.ua/archive/2018/volume4/issue3/oJVMBBS_2018043_031.pdf
Introduction. Because of the
incessant ill situation of avian influenza worldwide
the circulation of influenza A virus among wild birds is constantly
monitored on the territory of Kazakhstan. Wild birds are a natural reservoir of
all known variants, including subtypes, of the human and animal influenza agent
that have caused all pandemics and large-scaled epizooties in the past.
The possible emergence of new
modified potentially dangerous variants of the virus stipulates the need of
complex monitoring of influenza in populations of wild birds, particularly at
the key points such as habitation areas and major migration routes.
Materials and methods. Field
samples. The biological material (cloacal swabs)
from near-water and terrestrial wild birds, that was
delivered from the ornithological station ‘Shakpak’
(Jambyl Region) and from Sorbulak
lake system (Almaty Region) in 2018, was used in the work.
Viral
RNA extraction. RNA of the influenza virus was
extracted with use of the TRI
Reagent, Sigma, following the manufacture’s
instructions.
PCR
amplification. Real time RT-PCR was
performed with the help of specific primers and a matrix gene probe (M+25, M-124 and probe M+64) and hemagglutinins (H5+1456, H5-1685 and probe Н5+1637)
with ‘OneStep RT-PCR
Kit’ of Qiagen Company. The amplification was carried out in ‘Light Cycler 2.0’ of Roche
Company following the manufacture’s
instructions.
Results and discussion. In
Influenza A virus was identified in 16.6% of samples, including the virus of
subtype H5 in 2.8% of samples from the Blyth’s
reed warblers (Acrocephalus dumetorum, Acrocephalidae) that inhabit Sorbulak
lake system (Almaty Region). In addition, the influenza A virus was
identified in single samples (1.3%) from Spanish sparrows (Passer hispaniolensis, Passeridae) inhabiting the ornithological station ‘Shakpak’ (Almaty Region).
Conclusion. The influenza A virus was detected in 17.9% of samples taken from wild
birds thus confirming their role in influenza A existence on the territory
of Kazakhstan.
Acknowledgments. The work was
implemented under the grant project ‘Molecular and epizootological
monitoring of avian influenza in Kazakhstan’ (2018–2020, AP05132659).
Keywords: monitoring, wild birds, influenza A virus, Kazakhstan
