Issue 4
Journal for Veterinary Medicine, Biotechnology and Biosafety
Volume
4, Issue 4, December 2018, Pages 12–15
ISSN 2411-3174 (print version) ISSN 2411-0388
(online version)
GENOTYPING OF FRANCISELLA TULARENSIS
ISOLATES FROM UKRAINE
Zlenko O. B. 1, Durr A. 2, Schwarz J. 2,
Vydaiko N. B. 3, Gerilovych A. P. 1
1 National Scientific Center ‘Institute of Experimental and
Clinical Veterinary Medicine’, Kharkiv, Ukraine, e-mail: oksana.ceratium@gmail.com
2 Bundeswehr Institute
of Microbiology, Munich, Germany
3 State Institution ‘Public Health Center of the
Ministry of Health of Ukraine’, Kyiv, Ukraine
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PDF (print version)
Citation for print version: Zlenko, O. B.,
Durr, A., Schwarz, J., Vydaiko, N. B.
and Gerilovych, A. P. (2018) ‘Genotyping
of Francisella tularensis
isolates from Ukraine’, Journal
for Veterinary Medicine, Biotechnology and Biosafety, 4(4),
pp. 12–15.
Download
PDF (online version)
Citation for online version: Zlenko, O. B.,
Durr, A., Schwarz, J., Vydaiko, N. B.
and Gerilovych, A. P. (2018)
‘Genotyping of Francisella tularensis
isolates from Ukraine’, Journal
for Veterinary Medicine, Biotechnology and Biosafety.
[Online] 4(4), pp. 12–15. Available at: http://jvmbbs.kharkov.ua/archive/2018/volume4/issue4/oJVMBBS_2018044_012-015.pdf
Summary. The aim of the work is to provide fingerprinting of
20 tularemia isolates collected at the territory of Ukraine and find their
relationships with other typed tularemia isolates. The 20 randomly sampled
Francisella tularensis thermolysates were collected
during 1997–2016 on the territory of Ukraine. The panel included samples
from 11 regions of Ukraine. Samples were collected
from patients (3 samples), rodents (8 samples) and lagomorphs
(1 sample), ticks (6 samples) and environment (2 samples). The
genotyping and processing of data was carried out using the MLVA
12+1 method. Data were statistically
processed using the method of unweighted pair
group method with arithmetic mean-clustering (UPGMA).
It was found that all specimens belong to the same subgenus F. tularensis
subsp. holarctica
and belong to three genotypes: Russian-Azerbaijan (5 samples),
Czechoslovakia (1 sample) and European (14 samples) one. The European
genotype is inherent in the 70% of studied samples almost in every studied year
and can be named as the leading genotype that
distributed on the territory of all Ukraine and is related to European
genotypes of F. tularensis.
Keywords: MLVA, tularemia, Ukraine, UPGMA,
Francisella tularensis
subsp. holarctica
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