Journal for Veterinary Medicine, Biotechnology and Biosafety
Volume
5, Issue 1, March 2019, Pages 10–12
ISSN 2411-3174 (print version) ISSN 2411-0388
(online version)
DOI: 10.36016/JVMBBS-2019-5-1-2
DETERMINATION OF THE
VITAMIN-MINERAL PREPARATION ‘ENERGOLIT’ STABILITY FOR THE TREATMENT
OF METABOLIC DISORDERS IN ANIMALS
Sachuk R. M.
Research Epizootology
Station of the Institute of Veterinary Medicine of the National Academy of
Agrarian Sciences of Ukraine, Rivne, Ukraine, e-mail: sachuk.08@ukr.net
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PDF (print version)
Citation for print version: Sachuk, R. M. (2019) ‘Determination
of the vitamin-mineral preparation ‘Energolit’ stability for the treatment
of metabolic disorders in animals’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 5(1),
pp. 10–12.
Download
PDF (online version)
Citation for online version: Sachuk, R. M. (2019) ‘Determination
of the vitamin-mineral preparation ‘Energolit’ stability for the
treatment of metabolic disorders in animals’, Journal for Veterinary Medicine, Biotechnology and Biosafety.
[Online] 5(1), pp. 10–12. DOI: 10.36016/JVMBBS-2019-5-1-2.
Summary. The aim of experiments was the study the shelf life
of the injectable preparation ‘Energolit’ when stored in vials of
light glass. Studies were carried out at a temperature within from +10 to
+25 °С and relative humidity (60 ± 5%).
Experiments were carried out in 3, 6, 9, 12, 18, 24 months, and one day
after the expiration date after the puncture of the stopper with a sterile
injection needle. The control was the newly prepared preparation. We checked
organoleptic parameters, pH, microbial contamination to assess the quality of
the ‘Energolit’. In addition, we determined the quantitative
content of vitamins B1, B2, B3, B5,
B6, and B12 by methods described in the ‘State
Pharmacopoeia of Ukraine’. Three tested batches of the preparation
‘Energolit’ showed constant composition throughout all period of
study during two years of storage, as well as one day after the puncture of the
stopper with a sterile injection needle in the primary packaging. Experimental
samples of the preparation were within possible variation during the tests on
changes of pH, identity and content of the vitamins B1, B2,
B3, B5, B6, and B12. Microbial
contamination (Escherichia coli, Staphylococcus aureus, Pseudomonas
aeruginosa) was not detected. Fungi contamination were not detected during
the experiment. Fungi contamination was less than 10 CFU/ml one day after
the puncture. The optimal storage terms of the preparation has been determined.
The findings indicated, that the shelf life of the preparation
‘Energolit’ is 2 years. The pH and mass fraction of the
vitamins B group were unchanged or were within the limits of possible
variation in the tested samples during the experiment. Microbial contamination
was not detected.
Keywords: B vitamins, stability, shelf life,
‘Energolit’
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