Journal for Veterinary Medicine, Biotechnology and Biosafety
Volume
5, Issue 1, March 2019, Pages 20–26
ISSN 2411-3174 (print version) ISSN 2411-0388
(online version)
DOI: 10.36016/JVMBBS-2019-5-1-5
PCR TEST SYSTEMS FOR THE CLAVICHLAMYDIA SALMONICOLA AND
PISCICHLAMYDIA SALMONIS
DETECTION IN FISH
Zezekalo V. K. 1, Peredera S. B. 1, Buslyk T. V. 2, Pochernyaev K. F. 2, Shcherbakova N. S. 1
1 Poltava State
Agrarian Academy, Poltava, Ukraine, e-mail: v.zezekalo@gmail.com
2 Institute of
Pig Breeding and Agroindustrial Production of the
National Academy of Agrarian Sciences, Poltava, Ukraine
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PDF (print version)
Citation for print version: Zezekalo, V. K.,
Peredera, S. B., Buslyk, T. V.,
Pochernyaev, K. F. and Shcherbakova, N. S.
(2019) ‘PCR test systems for the Clavichlamydia salmonicola and Piscichlamydia salmonis detection in fish’, Journal for Veterinary Medicine, Biotechnology and
Biosafety, 5(1), pp. 20–26.
Download
PDF (online version)
Citation for online version: Zezekalo, V. K.,
Peredera, S. B., Buslyk, T. V.,
Pochernyaev, K. F. and Shcherbakova, N. S.
(2019) ‘PCR test systems for the Clavichlamydia salmonicola and Piscichlamydia salmonis detection in fish’, Journal for Veterinary Medicine, Biotechnology and
Biosafety. [Online] 5(1), pp. 20–26. DOI: 10.36016/JVMBBS-2019-5-1-5.
Summary. The aim of our work was
to develop PCR test systems for the identification and differentiation of the Piscichlamydia salmonis
and Clavochlamydia salmonicola,
species, that are known epitheliocystis
infection agents of gill and fish skin diseases, characterized by the presence
of specific ‘inclusions’ in the epithelial cells of the gills. To
date, the diseases of fish associated with chlamydial infections have been detected in more than 90 species of
freshwater and marine fish worldwide. For now, there is no available
information on the prevalence of Piscichlamydia
salmonis and Clavochlamydia
salmonicola, which can cause epitheliocystis of commercially important
aquaculture species in Ukraine. Identification of these pathogens is possible
only using molecular genetic methods. As a result of
our research, we got PCR tests for the identification and species
differentiation of Piscichlamydia salmonis and Clavochlamydia
salmonicola. The use of diagnostics for the
identification of Piscichlamydia salmonis and Clavochlamydia
salmonicola makes chlamydial infections
monitoring among various fish species possible and it will increase the
economic efficiency of fish farms.
Keywords: Piscichlamydia salmonis, Clavochlamydia
salmonicola, chlamydial infections in fish, Ukraine, PCR, Atlantic salmon, Salmo salar, brown trout, Salmo trutta
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