Journal for Veterinary Medicine, Biotechnology and Biosafety
Volume
5, Issue 1, March 2019, Pages 27–33
ISSN 2411-3174 (print version) ISSN 2411-0388
(online version)
DOI: 10.36016/JVMBBS-2019-5-1-6
ACUTE AND SUB-ACUTE TOXICITY
ASSESSMENT OF ‘RYBOZURIL’ ON COMMON CARP
Yevtushenko A. V.,
Boyko V. S., Romanko M. Ye.
National Scientific Center
‘Institute of Experimental and Clinical Veterinary Medicine’, Kharkiv, Ukraine,
e-mail: aevt76@gmail.com
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PDF (print version)
Citation for print version: Yevtushenko, A. V., Boyko, V. S. and Romanko, M. Ye.
(2019) ‘Acute
and sub-acute toxicity assessment of ‘Rybozuril’ on common carp’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 5(1),
pp. 27–33.
Download
PDF (online version)
Citation for online version: Yevtushenko, A. V., Boyko, V. S. and Romanko, M. Ye.
(2019) ‘Acute
and sub-acute toxicity assessment of ‘Rybozuril’ on common carp’, Journal for Veterinary Medicine, Biotechnology and Biosafety.
[Online] 5(1), pp. 27–33. DOI: 10.36016/JVMBBS-2019-5-1-6.
Summary. The goal of the work was
to study of acute and sub-acute toxicity parameters of ‘Rybozuril’ biological product (AI — diclazuril) on the model of carp. This drug is effective in
the treatment of diseases caused by parasitic Eimeriidae.
Carp scales of two years old were used in experiments.
To determine acute toxicity, the fish were prescribed
with diclazuril in doses of 1 g/kg, 5, 10, and
15 g/kg of live weight. Two experimental and control fish groups of 30 individuals
each were formed to determine sub-acute toxicity of ‘Rybozuril’. Experimental groups of fish were prescribed with ‘Rybozuril’
in a dose (by AI) of 50 and 10 mg/kg for two consecutive days. Blood
samples were collected from six fish from each group for clinical and
biochemical indicators after 2, 7, 14, 21, and 28 days. The hemoglobin
content, number of red blood cells and leukocytes blood was determined. The
intensity of peroxide oxidation of lipids (PОL),
catalase activity, level of total antioxidant capacity (TAC),
total proteins, albumin, globulins and glucose, circulating immune complexes (CІC) and seromucoids
concentration, level of enzymatic activity: aspartate transaminase (АSТ), alanine transaminase (АLТ),
α-amylase blood plasma were determined. The acute toxicity of diclazuril for carp was estimated, LD50
is more than 15,000 mg/kg of live weight, the toxicity of diclazuril can be classified as undifferentiated and, in
terms of toxicity, it can be classified as hazard
class IV. Two administrations of the drug ‘Rybozuril’
in a daily dose of 50.0 mg/kg of live weight, the maximum expression of
metabolic changes in fish was detected from the initial terms of the studies
and up to day 21. According to the results the toxic effect of the drug in fish
was estimated, which did not influence to a number of
indicators. The drug in such dose was shown immunosuppression and membrane-toxic
effects in fish. Two-time administration of the ‘Rybozuril’
drug in a daily dose of 10.0 mg/kg body weight leads to metabolic
alterations in fish due to the activation of
detoxification processes and lipoperoxidation
maintenance in cell membranes at the physiological level. At the end of the experiment the toxic effect of ‘Rybozuril’
in fish characterized by stable parameters in comparing to the control group.
Keywords: diclazuril, fish, lethal dose, acute
toxicity, submerged toxicity, blood, clinical and biochemical
indicators
References:
Al-Akel, A. S., Al-Balawi, H. F. A., Al-Misned, F.,
Mahboob, S., Ahmad, Z. and Suliman, E. M. (2010) ‘Effects of dietary
copper exposure on accumulation, growth, and hematological parameters in Cyprinus carpio’,
Toxicological and Environmental Chemistry, 92(10),
pp. 1865–1878. http://dx.doi.org/10.1080/02772248.2010.486230
CEC (The Council of the European Communities). (1986) ‘Council
Directive 86/609/EEC of 24 November 1986 on the approximation of laws,
regulations and administrative provisions of the Member States regarding the
protection of animals used for experimental and other scientific
purposes’, The Official Journal of
the European Communities, L 358, pp. 1–28. Available at: http://data.europa.eu/eli/dir/1986/609/oj
Conway, D. P., Mathis, G. F., Johnson, J.,
Schwartz, M. and Baldwin, C. (2001) ‘Efficacy of diclazuril in comparison with chemical and ionophorous anticoccidials
against Eimeria spp.
in broiler chickens in floor pens’, Poultry Science, 80(4), pp. 426–430.
http://dx.doi.org/10.1093/ps/80.4.426
De Bono, D. (1994) ‘Free radicals and antioxidants in
vascular biology: the roles of reaction kinetics, environment and substrate
turnover’, QJM: Monthly Journal of
the Association of Physicians, 89(8), pp. 445–453. http://dx.doi.org/10.1093/oxfordjournals.qjmed.a068954
Demidov, N. V.
and Berezkina, S. V. (1986) Guidelines for assessment of anthelmintics in veterinary medicine [Metodicheskie
rekomendatsii po otsenke angel’mintikov v veterinarii]. Moscow: VASKhNIL.
[in Russian]
Dirksen, G. (1990) ‘Liver [Leber]’,
in Dirksen, G., Gründer, H. D.
and Stöber, M. (eds.) Rosenberger, G. Clinical examination of cattle [Die klinische Untersuchung des Rindes]. 3rd ed.
Berlin, Hamburg: Paul Parey, pp. 367–384. ISBN
9783830440383. [in German]
EMEA
(European Medicines Agency) (1996) Diclazuril —
summary report (1). Report of the Committee for Veterinary Medicinal
Products. EMEA/MRL/086/96-FINAL.
Available at: https://www.ema.europa.eu/en/documents/mrl-report/diclazuril-summary-report-1-committee-veterinary-medicinal-products_en.pdf
FEEDAP (EFSA Panel on Additives and Products or Substances used in
Animal Feed) (2014) ‘Scientific Opinion on the safety and efficacy of Coxiril® (diclazuril)
as a feed additive for turkeys for fattening: Coxiril®
(diclazuril) for turkeys for fattening’, EFSA Journal, 12(6), p. 3729. http://dx.doi.org/10.2903/j.efsa.2014.3729
FEEDAP (EFSA Panel on Additives and Products or Substances used in
Animal Feed) (2015) ‘Scientific Opinion on the safety and efficacy of Coxiril® (diclazuril)
for rabbits for fattening and breeding: Coxiril®
(diclazuril) for rabbits for fattening and
breeding’, EFSA Journal, 13(1),
p. 3968. http://dx.doi.org/10.2903/j.efsa.2015.3968
Gavrilov, V. B.
and Mishkorudnaya, M. I. (1983)
‘Spectrophotometric determination of the content of lipid hydroperoxides in blood plasma’ [Spektrofotometricheskoe
opredelenie soderzhaniya gidroperekisey lipidov v plazme krovi], Laboratory Science [Laboratornoe
delo], 3,
pp. 33–36. [in Russian]
GOST 12.1.007-76.
Occupational safety standards system. Noxious
substances. Classification and general safety requirements [Sistema standartov bezopasnosti truda. Vrednye veshchestva. Klassifikatsiya i obshchie trebovaniya
bezopasnosti]. [in
Russian]
Khazanov, A. I.
(1988) Functional diagnosis of liver
diseases [Funktsional’naya diagnostika
bolezney pecheni]. 2nd ed. Moscow: Meditsina.
ISBN 522500069X. [in
Russian]
Klebanov, G. I.,
Babenkova, I. V., Teselkin, Yu. O.,
Komarov, O. S. and Vladimirov, Yu. A.
(1988) ‘Evaluation of the antioxidative activity of blood plasma using yolk
lipoproteins’ [Otsenka antiokislitel’noy aktivnosti
plazmy krovi s primeneniem zheltochnykh lipoproteidov], Laboratory
Science [Laboratornoe delo],
5, pp. 59–62. PMID: 2459459. [in Russian]
Kondrakhin, I. P.,
Kurilov, N. V., Malakhov, A. G.,
Arkhipov, A. V., Belov, A. D.,
Belyakov, I. M., Blinov, N. I.,
Korobov, A. V., Frolova, L. A.
and Sevast’yanova, N. A. (1985) Clinical laboratory diagnostics in
veterinary medicine [Klinicheskaya laboratornaya diagnostika v veterinarii]. Moscow: Agropromizdat.
[in Russian]
Korolyuk, M. A.,
Ivanova, L. I., Mayorova, I. G.
and Tokarev, V. E. (1988)
‘Determination of catalase activity’ [Opredelenie
aktivnosti katalaz], Laboratory Science [Laboratornoe
delo], 1, pp. 16–18. PMID: 2451064. [in Russian]
Kotsiumbas, I. Ya., Malyk, O. H., Patereha, I. P.,
Tishyn, O. L. and Kosenko, Yu. M.
(2006) Preclinical studies of veterinary
drugs [Doklinichni doslidzhennia
veterynarnykh likarskykh zasobiv]. Lviv: Triada plus.
ISBN 9667596648. [in Ukrainian]
Levinsky, R. J.
(1981) ‘Role of circulating immune complexes in renal diseases’, Journal
of Clinical Pathology, 34(11), pp. 1214–1222. http://dx.doi.org/10.1136/jcp.34.11.1214
Maltsev, V. N. (2012) ‘About
parasitological situation in carp hatcheries of the south regions of Ukraine’
[O parazitologicheskoy situatsii
v karpovykh rybopitomnikakh
yuzhnykh oblastey Ukrainy], Modern
Problems of Theoretical and Practical Ichthyology: Proceedings of the 5th
International Ichthyologic Scientific and Practical Conference (Chernivtsi, 13–16
September 2012) [Suchasni problemy
teoretychnoi i praktychnoi ikhtiolohii: materialy V mizhnarodnoi ikhtiolohichnoi naukovo-praktychnoi
konferentsii (Chernivtsi, 13–16 veresnia 2012 r.)].
Chernivtsi: Knyhy-XXI, pp. 142–148.
Available at: https://www.eco-tiras.org/docs/berg/Cernauti-kof_ichthyo_chernivtsi_2012.pdf.
[in Russian]
Marques, G. L., Neto, F. F.,
Ribeiro, C. A. de O., Liebel, S.,
Fraga, R. de and
Bueno, R. da R. L. (2015) ‘Oxidative damage in the
aging heart: an experimental rat model’, The Open Cardiovascular
Medicine Journal, 9(1), pp. 78–82. http://dx.doi.org/10.2174/1874192401509010078
Medved’, L. I.,
Kagan, Yu. S. and Spynu, Ye. I. (1968)
‘Pesticides and health problems’
[Pestitsidy i problemy zdravookhraneniya], Journal of the D. I. Mendeleev All-Union
Chemical Society [Zhurnal Vsesoyuznogo
khimicheskogo obshchestva imeni D. I. Mendeleeva], 13(3), pp. 263–271
Men’shikov, V. V. (ed.) (1987) Laboratory
research methods in the clinic [Laboratornye metody issledovaniya v klinike]. Moscow: Meditsina.
[in Russian]
Molnár, K. and Ostoros, G. (2007)
‘Efficacy of some anticoccidial drugs for
treating coccidial enteritis of the common carp
caused by Goussia carpelli
(Apicomplexa: Eimeriidae)’,
Acta Veterinaria Hungarica, 55(1), pp. 67–76. http://dx.doi.org/10.1556/AVet.55.2007.1.7
Selifanova, E. A.
and Birukova, N. P. (2012) ‘Subchronic toxicity of Eimeterm diclazuril’ [Subkhronicheskaya
toksichnost’ preparata
Eymeterm diclazuril], Theory and Practice of Struggle Against Parasitic Diseases [Teoriya
i praktika bor’by s parazitarnymi boleznyami], 13, pp. 385–387. Available at: https://elibrary.ru/item.asp?id=30311107. [in
Russian]
Tokarev, A. N.,
Selifanova, E. A., Napalkova, V. V.
and Zhuravleva, A. Z. (2012)
‘Comparative evaluation of acute toxicities of Eimeterm
diklazuril and Diacox’
[Sravnitel’naya otsenka
ostroy toksichnosti preparatov Eymeterm diklazurila i Diakoksa],
Theory and Practice of Struggle Against Parasitic Diseases [Teoriya
i praktika bor’by s parazitarnymi boleznyami], 13, pp. 418–421. Available at: https://elibrary.ru/item.asp?id=30311119. [in
Russian]
Trombitskiy, I. D.
and Bushuev, S. G. (eds.) (2011) Comprehensive Moldovan-Ukrainian studies of
the ichthyofauna of the Lower Dniester
basin —2011 [Kompleksnye moldo-ukrainskie issledovaniya ikhtiofauny vodoemov basseyna Nizhnego Dnestra — 2011]. Project report. Available
at: http://www.eco-tiras.org/docs/DniesterFishReport2011.pdf.
[in Russian]
Verheyen, A.,
Maes, L., Coussement, W.,
Vanparijs, O., Lauwers, F.,
Vlaminckx, E., Borgers, M.
and Marsboom, R. (1988) ‘In vivo action of
the anticoccidial diclazuril
(Clinacox®) on the developmental
stages of Eimeria tenella: an ultrastructural evaluation’, The Journal of
Parasitology, 74(6), pp. 939–949. http://dx.doi.org/10.2307/3282213
Vlizlo, V. V.
(ed.) (2012) Laboratory methods of
research in biology, animal husbandry and veterinary medicine [Laboratorni metody doslidzhen u biolohii, tvarynnytstvi ta veterynarnii medytsyni]. Lviv: Spolom. ISBN
9769666656776. [in Ukrainian]
