Journal for Veterinary Medicine, Biotechnology and Biosafety
Volume
5, Issue 3, September 2019, Pages 10–12
ISSN 2411-3174 (print version) ISSN 2411-0388
(online version)
PORCINE CIRCOVIRUS
TYPE II SCREENING IN FERAL SWINE POPULATION IN UKRAINE
Rudova N. G., Bolotin V. I.,
Solodiankin O. S., Gerilovych А. P.
National Scientific
Center ‘Institute of Experimental and Clinical Veterinary
Medicine’, Kharkiv, Ukraine, e-mail: rudovanatawa@ukr.net
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PDF (print version)
Citation for print version: Rudova, N. G.,
Bolotin, V. I., Solodiankin, O. S.
and Gerilovych, А. P. (2019) ‘Porcine
circovirus type II screening in feral swine
population in Ukraine’, Journal
for Veterinary Medicine, Biotechnology and Biosafety, 5(3),
pp. 10–12.
Download
PDF (online version)
Citation for online version: Rudova, N. G.,
Bolotin, V. I., Solodiankin, O. S.
and Gerilovych, А. P. (2019)
‘Porcine circovirus type II screening in feral
swine population in Ukraine’, Journal
for Veterinary Medicine, Biotechnology and Biosafety.
[Online] 5(3), pp. 10–12. DOI: 10.36016/JVMBBS-2019-5-3-2.
Summary. Porcine circovirus
type 2 (PCV2) is an emergent single-stranded DNA
virus found all over the world in domestic pigs and wild boars that causes
infectious disease with a great impact on swine productivity. PCV2 has 1.7 kb genome that includes two main genes,
which encode replication-related protein (rep)
and the major structural capsid (cap)
protein. Both of these genes can be used as target
sequences for the primer design for the detection of PCV2
as well as for sequencing of designated regions. We carried out a screening due
to the PCV2 circulating among the wild boar
population in 10 regions of Ukraine. PCR screening was
performed using primer pairs designed on the target sequences of the
replicative and capsid genes. According to the results of the research, the
presence of genetic material of PCV2 was found in 31.8% of the tested samples. The developed set
of primers may be suitable for diagnostics, as well as for the development of
specific sites for the purpose of sequencing of PCV2 cap-gene due
to the obtained DNA samples during epizootic screening
Keywords: PCV2, rep-gene,
cap-gene, epizootic screening, PCR
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