Journal for Veterinary Medicine, Biotechnology and Biosafety

Volume 5, Issue 4, December 2019, Pages 18–21

ISSN 2411-3174 (print version) ISSN 2411-0388 (online version)

ULTRASTRUCTURE AND BIOLOGICAL PROPERTIES OF AVIAN INFLUENZA VIRUSES FOLLOWING CRYOPRESERVATION

Stegniy M. Yu., Stegniy B. T.

National Scientific Center ‘Institute of Experimental and Clinical Veterinary Medicine’, Kharkiv, Ukraine, e-mail: marina.stegniy@gmail.com

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Citation for print version: Stegniy, M. Yu. and Stegniy, B. T. (2019) ‘Ultrastructure and biological properties of Avian influenza viruses following cryopreservation’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 5(4), pp. 18–21.

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Citation for online version: Stegniy, M. Yu. and Stegniy, B. T. (2019) ‘Ultrastructure and biological properties of Avian influenza viruses following cryopreservation’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 5(4), pp. 18–21. DOI: 10.36016/JVMBBS-2019-5-4-5.

Summary. Ultrastructure and infectious activity of avian influenza virus (strain А/Chicken/Sivash/02/05 (H5N1)) following cryopreservation and low temperature storage at –20, –70, and –196°C during various terms from 25 days up to 143 months using electron microscopy, serological and virological methods were investigated. Avian influenza viruses strain А/Chicken/Sivash/02/05 (H5N1) is stored in the Collection of Pathogens of the National Scientific Center ‘Institute of Experimental and Clinical Veterinary Medicine’ (Kharkiv, Ukraine), which was granted the National Endowment of Ukraine status. The conducted study allowed to reveal on electronograms the ultrastructural changes in AIV during long term storage (18 months) at moderately low temperature (–20°C), in particular loss of glycoprotein of peplomers in the majority of virions. The changes in ultrastructure of the virus samples were accompanied by a loss of hemagglutinating activity during long-term storage of AIV samples at moderately low temperature of –20°C. When storing the AIV samples at –70 and –196°C the virions generally remain negatively contrasted, keep peplomers for the studied storage duration

Keywords: avian influenza virus, morphology, ultrastructure, virion, cryopreservation, cryobank, cryodamage

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