Journal for Veterinary Medicine, Biotechnology and Biosafety
Volume
6, Issue 4, December 2020, Pages 5–8
ISSN 2411-3174 (print version) ISSN 2411-0388
(online version)
FIRST REPORT OF CANINE
BRUCELLOSIS IN UKRAINE: PATHOGEN ISOLATION AND CHARACTERIZATION
Bolotin V. I. 1,
Pikun O. Yu. 1, Marchenko N. V. 1,
Kozhevnik I. Ya. 2, Rudova N. G. 1,
Solodiankin O. S. 1, Stegniy B. T. 1,
Gerilovych A. P. 1
1 National
Scientific Center ‘Institute of Experimental and Clinical Veterinary
Medicine’, Kharkiv, Ukraine, e-mail: vbolotin@hotmail.de
2 Veterinary
Clinic ‘LapUsik’, Volnovakha, Donetsk Region, Ukraine
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PDF (print version)
Citation for print version: Bolotin, V. I.,
Pikun, O. Yu., Marchenko, N. V.,
Kozhevnik, I. Ya., Rudova, N. G.,
Solodiankin, O. S., Stegniy, B. T. and
Gerilovych, A. P. (2020) ‘First report of Canine brucellosis in
Ukraine: Pathogen isolation and characterization’, Journal for Veterinary Medicine, Biotechnology and
Biosafety, 6(4), pp. 5–8.
Download
PDF (online version)
Citation for online version: Bolotin, V. I.,
Pikun, O. Yu., Marchenko, N. V.,
Kozhevnik, I. Ya., Rudova, N. G.,
Solodiankin, O. S., Stegniy, B. T. and
Gerilovych, A. P. (2020) ‘First report of Canine brucellosis in
Ukraine: Pathogen isolation and characterization’, Journal for Veterinary Medicine, Biotechnology and
Biosafety. [Online] 6(3), pp. 5–8. DOI: 10.36016/JVMBBS-2020-6-4-1.
Summary. For the first time in Ukraine we confirmed canine
brucellosis caused by Brucella canis. The bacterium was isolated from
testicles of three-year-old male Labrador retriever with orchitis and
epididymitis. Initially blood serum sample was positive in cCFT, AGID and LFIA.
In addition to the pathogen isolation and identification by biochemical test
and PCR, the antimicrobial susceptibility test was performed that showed
sensitive of B. canis to the commonly used antibiotics, which
should be taken into account for the further therapy
Keywords: antibiotics, Bruce-ladder PCR, Brucella canis, orchitis, epididymitis,
serology
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