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Journal for Veterinary Medicine, Biotechnology and Biosafety

Volume 7, Issue 4, December 2021, Pages 19–25

ISSN 2411-3174 (print version) ISSN 2411-0388 (online version)

STUDY OF ISOLATE OF INFECTIOUS RYNOTRACHEITIS VIRUS IDENTIFIED IN THE ACUTE COURSE OF THE DISEASE

Kornieikov О. M. ¹, Stegniy M. Yu. ¹, Arefiev V. L. ¹, Oleshko А. Yu. ¹, Borodai N. І. ¹, Dunaiev Yu. K. ¹, Gerilovych A. P. ¹, Al Jabari M. ²

¹ National Scientific Center ‘Institute of Experimental and Clinical Veterinary Medicine’, Kharkiv, Ukraine, e-mail: korneykov@ukr.net

² State Biotechnological University, Kharkiv, Ukraine

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Citation for print version: Kornieikov, О. M., Stegniy, M. Yu., Arefiev, V. L., Oleshko, А. Yu., Borodai, N. І., Dunaiev, Yu. K., Gerilovych, A. P. and Al Jabari, M. (2021) ‘Study of isolate of Infectious rynotracheitis virus identified in the acute course of the disease’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 7(4), pp. 19–25.

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Citation for online version: Kornieikov, О. M., Stegniy, M. Yu., Arefiev, V. L., Oleshko, А. Yu., Borodai, N. І., Dunaiev, Yu. K., Gerilovych, A. P. and Al Jabari, M. (2021) ‘Study of isolate of Infectious rynotracheitis virus identified in the acute course of the disease’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 7(4), pp. 19–25. DOI: 10.36016/JVMBBS-2021-7-4-4.

Summary. Isolation of the virus from biological material from a two-month-old calf with pathology of the respiratory system from a herd with a morbidity rate of 48% was performed. After detection the presence of IRT antigens in the lungs of the dead animal, the pathogen was isolated on a continuous culture of calf kidney cells, where a characteristic cytopathic effect was observed. The genetic material of the bovine herpesvirus type 1 (Bovine herpesvirus-1, BHV-1) was identified by polymerase chain reaction in the test sample. The virus isolate was adapted to continuous cell cultures of calf kidneys, sheep kidney, cow embryo lung and calf trachea, and the most suitable biological system was determined, where adsorption and reproductive properties of the virus were more pronounced. It was found that the highest titer of infectious activity of BHV-1 isolate (6.1 lg TCD₅₀/cm³) was obtained on continuous culture of lung embryonic cells of a cow embryo after its reproduction during three consecutive passages (observation period)

Keywords: virus isolation, herpesvirus type 1, infectious activity, infectious rhinotracheitis, cell culture, cytopathic effect

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