Journal for Veterinary Medicine, Biotechnology and Biosafety

Volume 7, Issue 4, December 2021, Pages 26–30

ISSN 2411-3174 (print version) ISSN 2411-0388 (online version)

VIRUCIDAL ACTIVITY OF DISINFECTANT ‘BIOLAID’

Kovalenko V. L. 1, Chechet O. M. 2, Polupan I. M. 2

1 State Scientific Control Institute of Biotechnology and Strains of Microorganisms, Kyiv, Ukraine, e-mail: kovalenkodoktor@gmail.com

2 State Scientific and Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise, Kyiv, Ukraine

Download PDF (print version)

Citation for print version: Kovalenko, V. L., Chechet, O. M. and Polupan, I. M. (2021) ‘Virucidal activity of disinfectant ‘Biolaid’’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 7(4), pp. 26–30.

Download PDF (online version)

Citation for online version: Kovalenko, V. L., Chechet, O. M. and Polupan, I. M. (2021) ‘Virucidal activity of disinfectant ‘Biolaid’’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 7(4), pp. 26–30. DOI: 10.36016/JVMBBS-2021-7-4-5.

Summary. The article presents the results of the study of toxic and virucidal action of the disinfectant ‘Biolaid’, which includes hydrogen peroxide, lactic acid, and supralactic acid. The research was conducted following the ‘Methodical Approaches to the Control of Disinfectants for Veterinary Medicine’ (Kovalenko and Nedosiekov, 2011). Toxicity of the disinfectant ‘Biolaid’ was characterized in SPEV and BHK-21/C13 cell cultures (ATCC CCL-10). Determination of virucidal activity of the disinfectant ‘Biolaid’ was performed on models of Aujeszky’s disease virus (strain ‘Arsky’) and rabies virus (strain CVS-11, ATCC VR 959). The toxic effect of the drug ‘Biolaid’ was determined for concentrations of 2.0%, 1.5%, 1.0%, 0.5%, and 0.25% at exposures of 30 and 60 min in an incubator at 37°С. The virucidal effect of ‘Biolaid’ was determined for similar concentrations using working dilutions of viral suspensions: for Aujeszky’s disease virus — 4.0 CPE50/cm3, for rabies virus — 4.0 TCID50/cm3. The results of the study showed that the disinfectant ‘Biolaid’ is not toxic to SPEV and BHK-21/C13 cells in all test concentrations (2.0%, 1.5%, 1.0%, 0.5%, and 0.25%) at exposures of 30 and 60 min. Disinfectant ‘Biolaid’ has 100% virucidal activity against Aujeszky’s disease virus (strain ‘Arsky’) and rabies virus (strain CVS-11, ATCC VR 959) in all tested concentrations (2.0%, 1.5%, 1.0%, 0.5%, and 0.25%). The virucidal effect of these viruses was manifested at exposures of both 30 and 60 min. The obtained results give grounds to recommend disinfectant ‘Biolaid’ for disinfection of various livestock and poultry farms in case of detection of viral infections

Keywords: rabies virus, Aujeszky’s disease virus, virucidal activity, cell culture

References:

Addie, D. D., Boucraut-Baralon, C., Egberink, H., Frymus, T., Gruffydd-Jones, T., Hartmann, K., Horzinek, M. C., Hosie, M. J., Lloret, A., Lutz, H., Marsilio, F., Pennisi, M. G., Radford, A. D., Thiry, E., Truyen, U., Möstl, K. and European Advisory Board on Cat Diseases (2015) ‘Disinfectant choices in veterinary practices, shelters and households: ABCD guidelines on safe and effective disinfection for feline environments’, Journal of Feline Medicine and Surgery, 17(7), pp. 594–605. doi: 10.1177/1098612X15588450.

Cap, M., Vaudagna, S., Mozgovoj, M., Soteras, T., Sucari, A., Signorini, M. and Leotta, G. (2019) ‘Inactivation of Shiga toxin-producing Escherichia coli in fresh beef by electrolytically-generated hypochlorous acid, peroxyacetic acid, lactic acid and caprylic acid’, Meat Science, 157, p. 107886. doi: 10.1016/j.meatsci.2019.107886.

Cupo, K. L. and Beckstead, R. B. (2019) ‘An in vitro assay of disinfectants on the viability of Heterakis gallinarum eggs’, Avian Diseases, 63(3), p. 511. doi: 10.1637/11952-081418-ResNote.1.

Curran, E. T., Wilkinson, M. and Bradley, T. (2019) ‘Chemical disinfectants: Controversies regarding their use in low risk healthcare environments (part 1)’, Journal of Infection Prevention, 20(2), pp. 76–82. doi: 10.1177/1757177419828139.

Goyal, S. M., Chander, Y., Yezli, S. and Otter, J. A. (2014) ‘Evaluating the virucidal efficacy of hydrogen peroxide vapour’, Journal of Hospital Infection, 86(4), pp. 255–259. doi: 10.1016/j.jhin.2014.02.003.

Kovalenko V. L. and Nedosiekov V. V. (2011) Methodical Approaches to the Control of Disinfectants for Veterinary Medicine [Metodychni pidkhody kontroliu dezinfikuiuchykh zasobiv dlia veterynarnoi medytsyny]. Kyiv. [in Ukrainian].

Kovalenko, V. L., Kovalenko, P. L., Ponomarenko, G. V., Kukhtyn, M. D., Midyk, S. V., Horiuk, Yu. V. and Garkavenko, V. M. (2018) ‘Changes in lipid composition of Escherichia coli and Staphylococcus areus cells under the influence of disinfectants Barez®, Biochlor® and Geocide®’, Ukrainian Journal of Ecology, 8(1), pp. 547–550. doi: 10.15421/2018_248.

Kovalenko, V. L., Ponomarenko, G. V., Kukhtyn, M. D., Paliy, A. P., Bodnar, O. O., Rebenko, H. I., Kozytska, T. G., Makarevich, T. V., Ponomarenko, O. V. and Palii, A. P. (2020) ‘Evaluation of acute toxicity of the “Orgasept” disinfectant’, Ukrainian Journal of Ecology, 10(4), pp. 273–278. doi: 10.15421/2020_1982.

Melo, E. F., McElreath, J. S., Wilson, J. L., Lara, L. J. C., Cox, N. A. and Jordan, B. J. (2020) ‘Effects of a dry hydrogen peroxide disinfection system used in an egg cooler on hatchability and chick quality’, Poultry Science, 99(11), pp. 5487–5490. doi: 10.1016/j.psj.2020.05.050.

Paliy, A. P., Rodionova, K. O., Braginec, M. V., Paliy, A. P. and Nalivayko, L. I. (2018) ‘Sanitary-hygienic evaluation of meat processing enterprises productions and their sanation’, Ukrainian Journal of Ecology, 8(2), pp. 81-88. Available at: https://www.ujecology.com/articles/sanitaryhygienic-evaluation-of-meat-processing-enterprises-productions-and-their-sanation.pdf.

Ríos-Castillo, A. G., González-Rivas, F. and Rodríguez-Jerez, J. J. (2017) ‘Bactericidal efficacy of hydrogen peroxide-based disinfectants against Gram-positive and Gram-negative bacteria on stainless steel surfaces’, Journal of Food Science, 82(10), pp. 2351–2356. doi: 10.1111/1750-3841.13790.

Thomas, C., Schönknecht, A., Püning, C., Alter, T., Martin, A. and Bandick, N. (2020) ‘Effect of peracetic acid solutions and lactic acid on microorganisms in on-line reprocessing systems for chicken slaughter plants’, Journal of Food Protection, 83(4), pp. 615–620. doi: 10.4315/0362-028X.JFP-19-350.

Van Haute, S., López-Gálvez, F., Gómez-López, V. M., Eriksson, M., Devlieghere, F., Allende, A. and Sampers, I. (2015) ‘Methodology for modeling the disinfection efficiency of fresh-cut leafy vegetables wash water applied on peracetic acid combined with lactic acid’, International Journal of Food Microbiology, 208, pp. 102–113. doi: 10.1016/j.ijfoodmicro.2015.05.020.

Wlazlo, L., Drabik, K., Al-Shammari, K. I. A., Batkowska, J., Nowakowicz-Debek, B. and Gryzińska, M. (2020) ‘Use of reactive oxygen species (ozone, hydrogen peroxide) for disinfection of hatching eggs’, Poultry Science, 99(5), pp. 2478–2484. doi: 10.1016/j.psj.2019.12.039.