Journal for Veterinary Medicine, Biotechnology and Biosafety
Volume
8, Issue 1–2, May 2022, Pages 23–29
ISSN 2411-3174 (print version) ISSN 2411-0388
(online version)
STUDY OF THE SAFETY AND HARMLESSNESS
OF A DISINFECTANT IN LABORATORY ANIMALS
Chechet O. M. 1,
Kovalenko V. L. 2
1 State Scientific and Research Institute of
Laboratory Diagnostics and Veterinary and Sanitary Expertise, Kyiv, Ukraine
2 State Scientific Control Institute of
Biotechnology and Strains of Microorganisms, Kyiv, Ukraine, e-mail: kovalenkodoktor@gmail.com
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PDF (print version)
Citation for print version: Chechet, O. M. and Kovalenko, V. L.
(2022) ‘Study of the safety and harmlessness of a disinfectant in
laboratory animals’, Journal
for Veterinary Medicine, Biotechnology and Biosafety, 8(1–2),
pp. 23–29.
Download
PDF (online version)
Citation for online version: Chechet, O. M. and Kovalenko, V. L.
(2022) ‘Study of the safety and harmlessness of a disinfectant in
laboratory animals’, Journal
for Veterinary Medicine, Biotechnology and Biosafety.
[Online] 8(1–2), pp. 23–29. DOI: 10.36016/JVMBBS-2022-8-1-2-4.
Summary. The work aimed to investigate the effect of the
disinfectant ‘Diolaid’ based on sodium chlorite and sodium chloride
on acute toxicity indicators, as well as on blood parameters of laboratory
animals. The experiments were carried out on 6-month-old clinically healthy
male rats (5 groups, 6 animals in each group, n = 30) and
female rats (5 groups, 6 animals in each group, n = 30)
weighing 200–220 g. The drug was administered to animals
intragastrically (by probe) and aerosol treatment of cells with animals was
carried out. Separately we studied the skin-irritating and sensitizing action
of the disinfectant ‘Diolaid’ on the groups of clinically healthy
guinea pigs and rats weighing 250–300 g by a daily application on
their back and sides of different concentrations of the drug for 30 days
for 30 min periods. In addition, we tested the effect of
‘Diolaid’ on nonspecific immune response indicators of these animal
species (bactericidal activity of blood serum, level of circulating immune
complexes, T and B cells, etc.). The
work used modern humane methods of care and use of laboratory animals. It was
found that after intragastric administration of ‘Diolaid’, the
average lethal dose (LD50) for male rats was 182 mg/kg of body
weight, and for female rats it was 170 mg/kg. It has been proven that the
drug has a temporary irritating and sensitizing effect and does not adversely
affect the parameters of hematopoiesis and non-specific immune response in the
form of a 0.06% solution. The research results indicate the low toxicity of the
‘Diolaid’ drug for laboratory animals and the possibility of its
use in low concentrations both for treating cages in the presence of animals
and for treating the animals themselves. For disinfection of water during its
storage in containers, we used the concentration of the ‘Diolaid’
drug (by chlorine dioxide) of 0.5–2 mg/l (0.0002–0.0008%),
depending on the degree of purity of the water to be treated. Such
concentrations ensure compliance of the chlorite residual concentrations with
hygienic standards
Keywords: rats, guinea pigs, acute toxicity, irritating
and sensitizing effects, immune response
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