• Home
• Archive
• Rules for Authors
• Policies on Conflict of Interest, Human and animal rights, and Informed Consent
• Peer reviewering policy
• Editorial Board
• Certificate
• Editorial Ethics
• Contacts

Journal for Veterinary Medicine, Biotechnology and Biosafety

Volume 9, Issue 4, December 2023, Pages 3–6

ISSN 2411-3174 (print version) ISSN 2411-0388 (online version)

STUDY OF THE SPREAD OF MINOR VIRAL CATTLE INFECTIONS (LEUKEMIA, IMMUNODEFICIENCY, AND SPUMAVIRUS INFECTION) USING POLYMERASE CHAIN REACTION

Biloivan O. V., Didyk T. B., Yurko P. S., Korneikova O. B., Paliy A. P., Gorbatenko S. K., Bryl N. F.

National Scientific Center ‘Institute of Experimental and Clinical Veterinary Medicine’, Kharkiv, Ukraine, e-mail: silverscreen91@gmail.com

Download PDF (print version)

Citation for print version: Biloivan, O. V., Didyk, T. B., Yurko, P. S., Korneikova, O. B., Paliy, A. P., Gorbatenko, S. K. and Bryl, N. F. (2023) ‘Study of the spread of minor viral cattle infections (Leukemia, Immunodeficiency, and Spumavirus infection) using polymerase chain reaction’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 9(4), pp. 3–6.

Download PDF (online version)

Citation for online version: Biloivan, O. V., Didyk, T. B., Yurko, P. S., Korneikova, O. B., Paliy, A. P., Gorbatenko, S. K. and Bryl, N. F. (2023) ‘Study of the spread of minor viral cattle infections (Leukemia, Immunodeficiency, and Spumavirus infection) using polymerase chain reaction’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 9(4), pp. 3–6. DOI: 10.36016/JVMBBS-2023-9-4-1.

Summary. The aim of the study was to investigate the prevalence of minor cattle infections (leukemia, bovine immunodeficiency and spumavirus infection) using the polymerase chain reaction (PCR). Blood samples were collected from cows in conditionally leukemia-free farms in ten regions of Ukraine to determine the presence of these infections. The samples were examined via classical PCR to detect the genetic material of the specific fragment of the ENV gene of the leukemia virus using BLV-env-3/BLV-env-4 primers recommended by the OIE. To identify the proviral DNA of bovine foamy virus (BFV), primers Int 1-Int 2 and Int 3-Int 4 were used, and for the detection of bovine immunodeficiency virus (BIV) proviral DNA, a pair of primers RT_+(–) flanking the conservative domain of reverse transcriptase and a pair of primers flanking the pol gene of the BIV were selected. The situation concerning leukemia is most severe in Sumy and Kharkiv regions. A significant percentage of animals carrying the foamy virus was observed in farms in Kirovohrad, Kherson, Donetsk, and Kharkiv regions. Moreover, genetic material of the immunodeficiency virus was found in samples from Kirovohrad, Donetsk, and Kherson regions. These results indicate a significant prevalence of minor infections among cattle in Ukraine due to a lack of awareness among livestock workers, highlighting the necessity for comprehensive sanitary and preventive measures

Keywords: foamy virus, DNA, Ukraine

References:

Constable, P. D., Hinchcliff, K. W., Done, S. H. and Grünberg, W (2017) ‘Enzootic bovine leukosis (Bovine lymphosarcoma)’, in Veterinary Medicine: A Textbook of the Diseases of Cattle, Horses, Sheep, Pigs and Goats. 11^th ed. Elsevier, pp. 785–794. doi: 10.1016/B978-0-7020-5246-0.00011-5.

Fechner, H., Kurg, A., Geue, L., Blankenstein, P., Mewes, G., Ebner, D. and Beier, D. (1996) ‘Evaluation of polymerase chain reaction (PCR) application in diagnosis of bovine leukaemia virus (BLV) infection in naturally infected cattle’, Journal of Veterinary Medicine, Series B, 43(1–10), pp. 621–630. doi: 10.1111/j.1439-0450.1996.tb00361.x.

Gorbatenko, S. K., Kornieikova, O. B., Rudova, N. H., Dunaiev, Yu. K., Stegniy, B. T. and Kornieikov, O. M. (2023a) ‘Insufficiently studied minor viral infections in livestock of Ukraine’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 9(1–2), pp. 8–11. doi: 10.36016/JVMBBS-2023-9-1-2-2.

Gorbatenko, S. K., Rudova, N. G., Kornieikova, O. B., Stegniy, M. Yu., Kovalenko, L. V., Kuznetsova, O. V. and Miahkykh, N. V. (2023b) ‘Study the distribution and biological characteristics of field isolates of Bovine immunodeficiency and Spumavirus infection pathogens’ [Vyvchennia rozpovsiudzhennia ta biolohichnykh vlastyvostei polovykh izoliativ zbudnykiv imunodefitsytu i spumavirusnoi infektsii velykoi rohatoi khudoby], Veterinary Medicine [Veterynarna medytsyna], 109, pp. 61–66. doi: 10.36016/VM-2023-109-11. [in Ukrainian].

Krasnikova, E. S. and Larionova, O. S. (2014) ‘Biosafety of the animal origin products from cattle infected with bovine leukemia virus or bovine immunodeficiency virus’ [K voprosu o biologicheskoy bezopasnosti produktsii, poluchennoy ot zhivotnykh, infitsirovannykh virusami enzooticheskogo leykoza i immunodefitsita KRS], Vestnik veterinarii, 2, pp, 85–88. [in Russian].

Materniak-Kornas, M., Osiński, Z., Rudzki, M. and Kuźmak, J. (2017) ‘Development of a recombinant protein-based ELISA for detection of antibodies against bovine foamy virus’, Journal of Veterinary Research, 61(3), pp. 247–252. doi: 10.1515/jvetres-2017-0034.

Meas, S., Usui, T., Ohashi, K., Sugimoto, C. and Onuma, M. (2002) ‘Vertical transmission of bovine leukemia virus and bovine immunodeficiency virus in dairy cattle herds’, Veterinary Microbiology, 84(3), pp. 275–282. doi: 10.1016/S0378-1135(01)00458-8.

Moody, C. A., Pharr, G. T., Murphey, J., Hughlett, M. B., Weaver, C. C., Nelson, P. D. and Coats, K. S. (2002) ‘Confirmation of vertical transmission of bovine immunodeficiency virus in naturally infected dairy cattle using the polymerase chain reaction’, Journal of Veterinary Diagnostic Investigation, 14(2), pp. 113–119. doi: 10.1177/104063870201400204.

Murray, S. M., Picker, L. J., Axthelm, M. K. and Linial, M. L. (2006) ‘Expanded tissue targets for foamy virus replication with simian immunodeficiency virus-induced immunosuppression’, Journal of Virology, 80(2), pp. 663–670. doi: 10.1128/JVI.80.2.663-670.2006.

OIE (World Organisation for Animal Health). (2018) ‘Chapter 3.4.9. Enzootic Bovine Leukosis’, in Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. 12^th ed. [version adopted in 2018]. Paris: WOAH. Available at: https://www.woah.org/fileadmin/Home/eng/Health_standards/tahm/3.04.09_EBL.pdf.

Orr, K. A., O’Reilly, K. L. and Scholl, D. T. (2003) ‘Estimation of sensitivity and specificity of two diagnostics tests for bovine immunodeficiency virus using Bayesian techniques’, Preventive Veterinary Medicine, 61(2), pp. 79–89. doi: 10.1016/j.prevetmed.2003.08.001.

Romen, F., Backes, P., Materniak, M., Sting, R., Vahlenkamp, T. W., Riebe, R., Pawlita, M., Kuzmak, J. and Löchelt, M. (2007) ‘Serological detection systems for identification of cows shedding bovine foamy virus via milk’, Virology, 364(1), pp. 123–131. doi: 10.1016/j.virol.2007.03.009.

Scobie, L., Jarrett, O., Venables, C., Sayers, A. R. and Weightman, S. (2001) ‘Prevalence of bovine immunodeficiency virus infection in cattle in Great Britain’, Veterinary Record, 149(15), pp. 459–460. doi: 10.1136/vr.149.15.459.

Straub, O. and Lévy, D. (1999) ‘Bovine immunodeficiency virus and analogies with human immunodeficiency virus’, Leukemia, 13(S1), pp. S106–S109. doi: 10.1038/sj.leu.2401324.