Journal for Veterinary Medicine, Biotechnology and Biosafety
Volume
10, Issue 1, March 2024, Pages 20–24
ISSN 2411-3174 (print version) ISSN 2411-0388
(online version)
DEVELOPMENT OF
TECHNOLOGY FOR THE PRODUCTION OF SYMBIOTIC BIOLOGICALLY ACTIVE SUPPLEMENT FOR ANIMALS BASED ON LACTOBACILLUS AND BIFIDOBACTERIUM
Gujvinska S. O.
National Scientific
Center ‘Institute of Experimental and Clinical Veterinary
Medicine’, Kharkiv, Ukraine, e-mail: biotechvet2024@gmail.com
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PDF (print version)
Citation for print version: Gujvinska, S. O.
(2024) ‘Development of
technology for the production of symbiotic biologically active supplement for animals based on Lactobacillus and Bifidobacterium’,
Journal for Veterinary Medicine,
Biotechnology and Biosafety, 10(1), pp. 20–24.
Download
PDF (online version)
Citation for online version: Gujvinska, S. O.
(2024) ‘Development of
technology for the production of symbiotic biologically active supplement for animals based on Lactobacillus and Bifidobacterium’,
Journal for Veterinary Medicine,
Biotechnology and Biosafety. [Online] 10(1), pp. 20–24. DOI: 10.36016/JVMBBS-2024-10-1-4.
Summary. The study aimed to develop a technological
procedure for the production of a symbiotic biologically active supplement for animals based on Lactobacillus
and Bifidobacterium. Three pilot batches of the symbiotic preparation were manufactured. The technology for the production of a
symbiotic biologically active supplement for animals has been
developed. The scheme of the technological process of
manufacturing a symbiotic is proposed: production of nutrient media and working
solutions; cultivation of cultures of lacto- and bifidobacteria for the
preparation of a symbiotic biological supplement for animals; freeze-drying of
cultures of lacto- and bifidobacteria for a symbiotic biological supplement for
animals; obtaining mass for a symbiotic biological supplement for animals;
control of the finished product before release; packaging, labeling,
transportation and storage of a symbiotic biological supplement for animals.
The formulation of a symbiotic biologically active supplement is proposed: a mixture of freeze-dried cultures of Lactobacillus
plantarum No. 7-317
and Bifidobacterium adolescentis
No. 17-316 (55–65%), inulin (1.0–2.5%), lactose
(1.0–2.5%), fructose (1.0–2.5%), starch (42–27.5%). The
number of microbial cells per 1 cm3 of
symbiotic is lactobacilli ≥ 108, bifidobacteria
≥ 108
Keywords: Lactobacillus plantarum No. 7-317, Bifidobacterium
adolescentis No. 17-316, freeze-drying
References:
Candela, M.,
Maccaferri, S., Turroni, S., Carnevali, P. and Brigidi, P.
(2010) ‘Functional intestinal microbiome, new frontiers in prebiotic
design’, International Journal of Food Microbiology,
140(2–3), pp. 93–101. doi: 10.1016/j.ijfoodmicro.2010.04.017.
CE (The Council
of Europe). (1986) European Convention
for the Protection of Vertebrate Animals Used for Experimental and Other
Scientific Purposes. (European Treaty Series, No. 123). Strasbourg:
The Council of Europe. Available at: https://conventions.coe.int/treaty/en/treaties/html/123.htm.
CEC (The Council
of the European Communities) (2010) ‘Directive 2010/63/EU of the European
Parliament and of the Council of 22 September 2010 on the protection of
animals used for scientific purposes’, The Official Journal of the European Communities, L 276,
pp. 33–79. Available at: http://data.europa.eu/eli/dir/2010/63/oj.
DSSU [State
Committee for Technical Regulation and Consumer Policy] (2005) DSTU 4483:2005. Veterinary
Immunobiological Preparations. Methods for Determination of Bacterial and
Fungous Contamination [Preparaty veterynarni imunobiolohichni. Metody
vyznachannia bakterialnoi i hrybkovoi kontaminatsii]. Kyiv:
Derzhspozhyvstandart Ukrainy. [in Ukrainian].
Floch, M. H.,
Walker, W. A., Madsen, K., Sanders, M. E.,
Macfarlane, G. T., Flint, H. J., Dieleman, L. A.,
Ringel, Y., Guandalini, S., Kelly, C. P. and
Brandt, L. J. (2011) ‘Recommendations for probiotic
use—2011 Update’, Journal of Clinical Gastroenterology, 45,
pp. 168–171. doi: 10.1097/MCG.0b013e318230928b.
Gujvinska, S. O.
(2015) ‘Determination of storage stability of the probiotics’ [Vyznachennia
stabilnosti probiotyka u protsesi zberihannia], Veterinary Medicine
[Veterynarna medytsyna], 101, pp. 208–211. Available at: https://jvm.kharkov.ua/sbornik/101/10_61.pdf. [in Ukrainian].
Gujvinska, S.
(2019) ‘Study of the biological properties of lactobacilli and
bifidobacteria’, 4th Annual BTRP Ukraine Regional One
Health Research Symposium, Kyiv, Ukraine, 20–24 May 2019: abstr.
Kyiv, p. 328.
Gujvinska, S. O.
and Paliy, A. P. (2018) ‘Determination of antagonistic and
adhesive properties of Lactobacterium
and Bifidobacterium’
[Vyznachennia antahonistychnykh ta adhezyvnykh vlastyvostei laktobakterii ta
bifidobakterii], Microbiological Journal [Mikrobiolohichnyi Zhurnal],
80(1), pp. 36–44. doi: 10.15407/microbiolj80.01.036. [in Ukrainian].
Khyzhniak, O. S. (2017) Development of the Composition and Biotechnology of the Multipurpose
Probiotic Preparation [Rozrobka skladu ta biotekhnolohii otrymannia
kompleksnoho probiotychnoho preparatu]. The dissertation thesis for the
scientific degree of the candidate of pharmaceutical sciences. Kharkiv: National Pharmaceutical University. Available at: https://nrat.ukrintei.ua/searchdoc/0417U002705. [in
Ukrainian].
Kianifar, H.,
Ahanchian, H., Grover, Z., Jafari, S., Noorbakhsh, Z.,
Khakshour, A., Sedaghat, M. and Kiani, M. (2014)
‘Synbiotic in the management of infantile colic: A randomised
controlled trial’, Journal of Paediatrics and Child Health,
50(10), pp. 801–805. doi: 10.1111/jpc.12640.
Krupytska, L. O.
and Kaprelyants, L. V. (2016) ‘Fatty acid composition of
dietary supplements with the inclusion of propionic acid bacteria’
[Zhyrnokyslotnyi sklad biolohichno aktyvnykh dobavok z vkliuchenniam
propionovokyslykh bakterii], Visnyk of
Lviv University. Biological Series [Visnyk Lvivskoho universytetu. Seriia
biolohichna], 73, p. 442. Available at: http://nbuv.gov.ua/UJRN/VLNU_biol_2016_73_123. [in Ukrainian].
Krupytska, L. O.,
Kaprelyants, L. V., Kylymenchuk, O. O. and
Velichko, T. O. (2018) ‘Non-waste biotechnology of obtaining a
symbiotic and a metabolite on the basis of Bifidobacterium
longum – Ya 3 and Propionibacterium
shermanii – 4’ [Bezvidkhodna biotekhnolohiia otrymannia
symbiotyka i metabiotyka na osnovi Bifidobacterium
longum – Ya 3 ta Propionibacterium
shermanii – 4], Scientific Messenger of Lviv National
University of Veterinary Medicine and Biotechnologies named after
S. Z. Gzhytskyj. Series: Food Technologies
[Naukovyi visnyk Lvivskoho natsionalnoho universytetu veterynarnoi medytsyny ta
biotekhnolohii imeni S. Z. Gzhytskoho. Seriia: Kharchovi tekhnolohii], 20(85), pp. 148–154. doi:
10.15421/nvlvet8527. [in Ukrainian].
Maydeo, A.
(2010) ‘Lactitol or lactulose in the treatment of chronic constipation:
Result of a systematic’, Journal of the Indian Medical Association,
108(11), pp. 789–792. PMID: 21510584.
Ohland, C. L.
and MacNaughton, W. K. (2010) ‘Probiotic bacteria and
intestinal epithelial barrier function’, American Journal of
Physiology. Gastrointestinal and Liver Physiology, 298(6),
pp. 807–819. doi: 10.1152/ajpgi.00243.2009.
Roberfroid, M. B.
(2000) ‘Prebiotics and probiotics: Are they functional foods?’, The American Journal of Clinical Nutrition,
71(6), pp. 1682-1687. doi: 10.1093/ajcn/71.6.1682S.
Samuylenko, A. Ya.,
Shkolnikov, Ye. E., Pavlenko, I. V.,
Rayewsky, A. A., Eremets, V. I., Skichko, N. D.,
Anisimova, L. V. and Andrianova, E. N. (2011)
‘Development of symbiotic lysine-synthesizing preparation for growing
broiler chickens’ [Razrabotka simbioticheskogo preparata na osnove E. coli VL-613 i primenenie ego v
broylernom ptitsevodstve], Veterinary Medicine [Veterynarna medytsyna],
95, pp. 183–185. Available at: http://nbuv.gov.ua/UJRN/vetmed_2011_95_80. [in Russian].
Simmonds, R. C. (2017)
‘Chapter 4. Bioethics and animal use in programs of research,
teaching, and testing’, in Weichbrod, R. H.,
Thompson, G. A. and Norton, J. N. (eds.) Management of
Animal Care and Use Programs in Research, Education, and Testing. 2nd ed. Boca Raton: CRC Press,
pp. 35–62. doi: 10.1201/9781315152189-4.
Starovoitova, S. O.,
Skrotska, O. I., Penchuk, Yu. M. and Pyroh, T. P.
(2012) The Technology of Probiotics
[Tekhnolohiia probiotykiv]. Kyiv: National University of Food Technologies.
ISBN 9789666121274. Available at: https://dspace.nuft.edu.ua/handle/123456789/10384. [in
Ukrainian].
Van Emden, H. F.
(2019) Statistics for Terrified
Biologists. 2nd ed. Hoboken, NJ:
John Wiley & Sons. ISBN 9781119563679.
Vastano, V.,
Capri, U., Candela, M., Siciliano, R. A., Russo, L.,
Renda, M. and Sacco, M. (2013) ‘Identification of binding sites
of Lactobacillus plantarum enolase involved in the interaction with human plasminogen’,
Microbiological Research, 168(2), pp. 65–72. doi: 10.1016/j.micres.2012.10.001.
Vitali, B.,
Ndagijimana, M., Cruciani, F., Carnevali, P., Candela, M.,
Guerzoni, M. E. and Brigidi, P. (2010) ‘Impact of a synbiotic
food on the gut microbial ecology and metabolic profiles’, BMC
Microbiology, 10(1), p. 4. doi: 10.1186/1471-2180-10-4.
VRU (Verkhovna
Rada Ukrainy) (2006) ‘Law of Ukraine No. 3447-IV of
