Journal for Veterinary Medicine, Biotechnology and Biosafety

Volume 10, Issue 1, March 2024, Pages 25–32

ISSN 2411-3174 (print version) ISSN 2411-0388 (online version)

SOME BIOLOGICAL PROPERTIES OF СHLORIRIDOVIRUS FROM CULISETA MOSQUITOES (DIPTERA: CULICIDAE)

Buchatsky L. P., Filenko O. M., Sutugina L. P.

Taras Shevchenko National University of Kyiv, Kyiv, Ukraine, e-mail: iridolpb@gmail.com

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Citation for print version: Buchatsky, L. P., Filenko, O. M. and Sutugina, L. P. (2024) ‘Some biological properties of сhloriridovirus from Culiseta mosquitoes (Diptera: Culicidae)’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 10(1), pp. 25–32.

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Citation for online version: Buchatsky, L. P., Filenko, O. M. and Sutugina, L. P. (2024) ‘Some biological properties of сhloriridovirus from Culiseta mosquitoes (Diptera: Culicidae)’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 10(1), pp. 25–32. DOI: 10.36016/JVMBBS-2024-10-1-5.

Summary. Two strains of mosquito iridescent viruses (Chloriridovirus) were isolated in Ukraine from seek larvae of bloodsucking mosquitoes Culiseta annulata (Schrank, 1776) and Culiseta morsitans (Theobald, 1901). Electron microscopic study of tissues from these larvae revealed icosahedral virus particles ranging from 180 nm to 5 nm in diameter, containing dense, pleomorphic nucleotides. Viruses were assembled in the cytoplasm within spherical virosomes. Both viruses contained DNA and 11 polypeptides with molecular weights varying from 16 kD to 98 kD. A DNA restriction analysis of both strains of mosquito chloriridovirus of the genus Culiseta and cloning of fragments of their genomes by genetic engineering methods was performed. Isolated strains were sensitive to ultraviolet insolation and heating and were stable to organic solvents such as ether and chloroform. Both isolated strains grew well in mosquito (Aedes aegypti, Aedes pseudoscutellaris, Aedes albopictus) and in Lepidoptera (Euxoa scandens, Antheraea pernyi) cell lines. A close antigenic relationship has been found between isolated strains and Chloriridovirus from Aedes cantans. Some antigenic relationship was also demonstrated between isolated strains and still unclassified iridovirus from carp (Cyprinus carpio). These findings imply that both strains share some similarity in structural and biochemical characteristics and belong to the Chloriridovirus genus of the Iridoviridae family

Keywords: electron microscopy, carp, Iridoviridae

References:

Bailey, G. S. (1996) ‘Ouchterlony double immunodiffusion’, in Walker, J. M. (ed.) The Protein Protocols Handbook. Totowa, NJ: Humana Press, pp. 749–752. doi: 10.1007/978-1-60327-259-9_135.

Balange-Orange, N. and Devauchelle, G. (1982) ‘Lipid composition of an iridescent virus type 6 (CIV)’, Archives of Virology, 73(3–4), pp. 363–367. doi: 10.1007/BF01318091.

Bowen, R. M. (1976) ‘Part 1 — Theory of mixtures’, in Eringen, A. C. (ed.) Continuum Physics, Volume III: Mixtures and EM Field Theories. New York: Academic Press, pp. 1–127. doi: 10.1016/B978-0-12-240803-8.50017-7.

Buchatsky, L. P. (1975) ‘Viruses pathogenic for mosquitoes (Diptera, Culicidae)’ [Virusy, patogennye dlya komarov (Diptera, Culicidae)], Problems of Virology [Voprosy virusologii], 3, pp. 268–273. PMID: 240230. [in Russian].

Buchatsky, L. P. (1977) ‘An iridovirus from larvae of Culiseta annulata and Culex territans’, Acta Virologica, 21(1), pp. 85–86. PMID: 15443.

Buchatsky, L. P. and Sherban, S. D. (1976) ‘Various biological properties of Aedes cantans irides iridescent virus’ [Nekotorye biologicheskyie svoistva iridovirusa komarov iz Aedes cantans], Problems of Virology [Voprosy virusologii], 4, pp. 432–435. PMID: 1007219. [in Russian].

Buchatsky, L. P. and Sheremet, V. P. (1974) ‘Detection of iridoviruses in mosquitoes in the Ukraine’ [Vyyavlenie iridovirusa komarov v Ukraine], Problems of Virology [Voprosy virusologii], 2, pp. 226–228. PMID: 4446562. [in Russian].

Buchatsky, L. P., Kaniuka, V. I. and Lebedinets, N. N. (1976) ‘Sensitivity of the honeycomb moth to mosquito iridescent virus’ [Chuvstvitel’nostbol’shoy voshchinnoi moli k iridovirusu komarov], Microbiology Journal [Mikrobiolohichnyi Zhurnal], 38(5), pp. 605–607. PMID: 1012084. [in Russian].

Buchatsky, L. P., Kuznetsova, M. A. and Prima, V. I. (1983) ‘Isolation and study of the physicochemical properties of the DNA of the iridescent virus from the mosquito Aedes cantans’ [Izolatsiya i izuchenie DNK iz iridovirusa komarov Aedes cantans], Problems of Virology [Voprosy virusologii], 3, pp. 355–358. PMID: 6613094. [in Russian].

Buchatsky, L. P., Kuznetsova, M. A. and Sherban, S. D. (1982) ‘Investigation of mosquito iridescent virus proteins’ [Izuchenie belkov iridovirusa komarov], Microbiology Journal [Mikrobiolohichnyi Zhurnal], 44(6), pp. 77–79. [in Russian].

Buchatsky, L. P., Victorov-Nabokov, O. V. and Sheremet, V. P. (1976) ‘New hosts of the mosquito iridescent virus in the Ukraine and in Karelia’ [Novye khozyaeva iridovirusa komarov na Ukraine i v Karelii], Microbiology Journal [Mikrobiolohichnyi Zhurnal], 38(4), pp. 502–505. PMID: 11396. [in Russian].

Carter, J. B. (1975) ‘The effect of temperature upon Tipula iridescent virus infection in Tipula oleracea’, Journal of Invertebrate Pathology, 25(1), pp. 115–124. doi: 10.1016/0022-2011(75)90291-8.

Cerutti, M. and Devauchelle, G. (1979) ‘Cell fusion induced by an invertebrate virus’, Archives of Virology, 61(1–2), pp. 149–155. doi: 10.1007/BF01320599.

Chapman, H. C., Clark, T. B., Woodard, D. B. and Kellen, W. R. (1966) ‘Additional mosquito hosts of the Mosquito Iridescent Virus’, Journal of Invertebrate Pathology, 8(4), pp. 545–546. doi: 10.1016/0022-2011(66)90089-9.

Chinchar, V. G., Hick, P., Ince, I. A., Jancovich, J. K., Marschang, R., Qin, Q., Subramaniam, K., Waltzek, T. B., Whittington, R., Williams, T., Zhang, Q.-Y., and ICTV Report Consortium (2017) ‘ICTV Virus Taxonomy Profile: Iridoviridae’, Journal of General Virology, 98(5), pp. 890–891. doi: 10.1099/jgv.0.000818.

Clark, T. B., Kellen, W. R. and Lum, P. T. M. (1965) ‘A mosquito iridescent virus (MIV) from Aedes taeniorhynchus (Wiedemann)’, Journal of Invertebrate Pathology, 7(4), pp. 519–521. doi: 10.1016/0022-2011(65)90133-3.

Cunningham, J. C. and Tinsley, T. W. (1968) ‘A serological comparison of some iridescent non-occluded insect viruses’, Journal of General Virology, 3(1), pp. 1–8. doi: 10.1099/0022-1317-3-1-1.

Devauchelle, G., Attias, J., Monnier, C., Barray, S., Cerutti, M., Guerillon, J. and Orange-Balange, N. (1985) ‘Chilo iridescent virus’, in Willis, D. B. (ed.) Iridoviridae. (Current Topics in Microbiology and Immunology, Vol. 116). Berlin, Heidelberg: Springer, pp. 37–48. doi: 10.1007/978-3-642-70280-8_3.

Elliott, R. M., Lescott, T. and Kelly, D. C. (1977) ‘Serological relationships of an iridescent virus (type 25) recently isolated from Tipula sp. with two other iridescent viruses (types 2 and 22)’, Virology, 81(2), pp. 309–316. doi: 10.1016/0042-6822(77)90147-7.

Filenko, O. M., Lebedinetz, N. N. and Buchatsky, L. P. (1995) ‘Antigenic affinity of mosquito iridoviruses’ [Antigennoe rodstvo iridovirusov komarov], Bulletin of Taras Shevchenko National University of Kiev. Series: Biology [Visnyk Kyivskoho natsionalnoho universytetu imeni Tarasa Shevchenka: Biolohiia], 26, pp. 139–146.

Hall, D. W. and Lowe, R. E. (1972) ‘Physical and serological comparisons of “R” and “T” strains of mosquito iridescent virus from Aedes taeniorhynchu’, Journal of Invertebrate Pathology, 19(3), pp. 317–324. doi: 10.1016/0022-2011(72)90228-5.

Huang, Y., Li, S., Zhao, Q., Pei, G., An, X., Guo, X., Zhou, H., Zhang, Z., Zhang, J. and Tong, Y. (2015) ‘Isolation and characterization of a novel invertebrate iridovirus from adult Anopheles minimus (AMIV) in China’, Journal of Invertebrate Pathology, 127, pp. 1–5. doi: 10.1016/j.jip.2015.01.007.

Kelly, D. C. and Vance, D. E. (1973) ‘The lipid content of two iridescent viruses’, Journal of General Virology, 21(2), pp. 417–423. doi: 10.1099/0022-1317-21-2-417.

Kelly, D. C., Ayres, M. D., Lescott, T., Robertson, J. S. and Happ, G. M. (1979) ‘A small iridescent virus (type 29) isolated from Tenebrio molitor: A comparison of its proteins and antigens with six other iridescent viruses’, Journal of General Virology, 42(1), pp. 95–105. doi: 10.1099/0022-1317-42-1-95.

Klump, H., Beaumais, J. and Devauchelle, G. (1983) ‘Structural and thermodynamic investigation of the Chilo iridescent virus (Iridovirus type 6)’, Archives of Virology, 75(4), pp. 269–276. doi: 10.1007/BF01314892.

Krell, P. and Lee, P. E. (1974) ‘Polypeptides in Tipula iridescent virus (TIV) and in TIV-infected hemocytes of Galleria mellonella (L.) larvae’, Virology, 60(2), pp. 315–326. doi: 10.1016/0042-6822(74)90327-4.

Marina, C. F., Arredondo-Jiménez, J. I., Castillo, A. and Williams, T. (1999) ‘Sublethal effects of iridovirus disease in a mosquito’, Oecologia, 119(3), pp. 383–388. doi: 10.1007/s004420050799.

Marina, C. F., Ibarra, J. E., ArredondoJiménez, J. I., FernándezSalas, I., Valle, J. and Williams, T. (2003) ‘Sublethal iridovirus disease of the mosquito Aedes aegypti is due to viral replication not cytotoxicity’, Medical and Veterinary Entomology, 17(2), pp. 187–194. doi: 10.1046/j.1365-2915.2003.00422.x.

Muttis, E., Micieli, M. V. and García, J. J. (2023) ‘Pathology of an iridescent virus in immature Culex pipiens L. (Diptera, Culicidae)’, Anais da Academia Brasileira de Ciências, 95(4), p. e20200558. doi: 10.1590/0001-3765202320200558.

Muttis, E., Miele, S. A. B., Belaich, M. N., Micieli, M. V., Becnel, J. J., Ghiringhelli, P. D. and García, J. J. (2012) ‘First record of a mosquito iridescent virus in Culex pipiens L. (Diptera: Culicidae)’, Archives of Virology, 157(8), pp. 1569–1571. doi: 10.1007/s00705-012-1302-2.

Piégu, B., Guizard, S., Spears, T., Cruaud, C., Couloux, A., Bideshi, D. K., Federici, B. A. and Bigot, Y. (2013) ‘Complete genome sequence of invertebrate iridescent virus 22 isolated from a blackfly larva’, Journal of General Virology, 94(9), pp. 2112–2116. doi: 10.1099/vir.0.054213-0.

Reed, L. J. and Mench, H. A. (1939) ‘A simple method for estimating fifty percent end point’, American Journal of Hygiene, 27, pp. 493–497.

Rud, Yu. P. and Buchatsky, L. P. (2009) ‘Experimental infection of the carp by mosquito iridovirus’ [Eksperymentalne infikuvannia koropa irydovirusom komara], Fisheries Science of Ukraine [Rybohospodarska nauka Ukrainy], 1, pp. 102–106. Available at: https://fsu.ua/index.php/en/2009/1-2009-7/2009-01-102-106. [in Ukrainian].

Schaffer, H. E. and Sederoff, R. R. (1981) ‘Improved estimation of DNA fragment lengths from agarose gels’, Analytical Biochemistry, 115(1), pp. 113–122. doi: 10.1016/0003-2697(81)90533-9.

Stoltz, D. B. (1971) ‘The structure of icosahedral cytoplasmic deoxyriboviruses’, Journal of Ultrastructure Research, 37(1–2), pp. 219–239. doi: 10.1016/S0022-5320(71)80052-7.

Sullivan, M. A., Yasbin, R. E. and Young, F. E. (1984) ‘New shuttle vectors for Bacillus subtilis and Escherichia coli which allow rapid detection of inserted fragments’, Gene, 29(1–2), pp. 21–26. doi: 10.1016/0378-1119(84)90161-6.

Tinsley, T. W., Robertson, J. S., Rivers, C. F. and Service, M. W. (1971) ‘An iridescent virus of Aedes cantans in Great Britain’, Journal of Invertebrate Pathology, 18(3), pp. 427–428. doi: 10.1016/0022-2011(71)90051-6.

TorybaevKh. K. and Dubitskiy, A. M. (1971) ‘Iridovirus in blood-sucking mosquitoes in southeast Kazakhstan’ [Raduzhnyy virus u krovososushchikh komarov na yugo-vostoke Kazakhstana], Bulletin of Agricultural Science of Kazakhstan [Vestnik sel’skokhozyaystvennoy nauki Kazakhstana], 12, pp. 101–102. [in Russian].

Wagner, G. W., Paschke, J. D., Campbell, W. R. and Webb, S. R. (1973) ‘Biochemical and biophysical properties of two strains of mosquito iridescent virus’, Virology, 52(1), pp. 72–80. doi: 10.1016/0042-6822(73)90399-1.

Weiser, J. (1965) ‘A new virus infection of mosquito larvae’, Bulletin of the World Health Organization, 33(4), pp. 586–588. PMID: 5295003.

Wesley, R. D. and Tuthill, A. E. (1984) ‘Genome relatedness among African swine fever virus field isolates by restriction endonuclease analysis’, Preventive Veterinary Medicine, 2(1–4), pp. 53–62. doi: 10.1016/0167-5877(84)90048-5.

Williams, T. (2008) ‘Natural invertebrate hosts of iridoviruses (Iridoviridae)’, Neotropical Entomology, 37(6), pp. 615–632. doi: 10.1590/S1519-566X2008000600001.

Wong, C. K., Young, V. L., Kleffmann, T. and Ward, V. K. (2011) ‘Genomic and proteomic analysis of invertebrate iridovirus type 9’, Journal of Virology, 85(15), pp. 7900–7911. doi: 10.1128/JVI.00645-11.

Yaremenko, D. M. and Buchatskyi, L. P. (2003) ‘Study of reproduction of iridovirus of gill necrosis of carp’ [Izuchenie reproduktsii virusa zhabernogo nekroza karpa], Veterinary Medicine of Ukraine [Veterynarna medytsyna Ukrainy], 3, pp. 29–30. [in Russian].