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Journal for Veterinary Medicine, Biotechnology and Biosafety

Volume 10, Issue 4, October 2024, Pages 40–45

ISSN 2411-3174 (print version) ISSN 2411-0388 (online version)

BACTERIOLOGICAL STUDIES OF PROBE SWABS WITH NASOPHARYNGEAL SECRETIONS FROM CANINES DIAGNOSED WITH BORDETELLOSIS

Hadzevych D. V.

National Scientific Center ‘Institute of Experimental and Clinical Veterinary Medicine’, Kharkiv, Ukraine, e-mail: d5195681@gmail.com

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Citation for print version: Hadzevych, D. V. (2024) ‘Bacteriological studies of probe swabs with nasopharyngeal secretions from canines diagnosed with Bordetellosis’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 10(4), pp. 40–45.

Download PDF (online version)

Citation for online version: Hadzevych, D. V. (2024) ‘Bacteriological studies of probe swabs with nasopharyngeal secretions from canines diagnosed with Bordetellosis’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 10(4), pp. 40–45. DOI: 10.36016/JVMBBS-2024-10-4-6.

Summary. At the present work was developed the greatest effectiveness of bacteriological investigations and detection of clinical isolates of Bordetella bronchiseptica from nasopharyngeal secretions was observed when the casein-charcoal agar (CCA) was frozen with 5% blood and cephalexin. Thus, during the first trial and sampling of animals, 12 (66.67%) positive results were recovered, and with repeated sampling and sampling of animals after 24 h and 48 h, 15 (83.33%) positive results were recovered from the total number of animals with bordetheliosis based on the results of PCR. The addition of cephalexin in the form of a selective component in nutrient media allows increasing the effectiveness and speed of bacteriological investigations by 2–4 doses per month, suppressing the growth of third-party nasopharyngeal microflora. This is a simple vision of pure culture. Repeated sampling and sampling of nasopharyngeal secretions from sick animals at a short interval of 24 h and 48 h allows us to obtain 16.66% more positive results from clinical isolates of B. bronchiseptica using the bacteriological method of investigation

Keywords: Bordetella bronchiseptica, tracheobronchitis, canine infectious respiratory disease complex

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